Corneal Sub‐Basal Nerve Plexus Assessment and its Association with Phenotypic Features and Lymphocyte Subsets in Sjögren's Syndrome
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.17/4283 |
Resumo: | Purpose: To assess and compare corneal sub-basal nerve plexus morphology with circulating lymphocyte subsets, immunologic status and disease activity in Sjögren syndrome (SjS) patients. Methods: Fifty-five SjS patients, 63 Sicca patients (not fulfilling SjS criteria), 18 rheumatoid arthritis (RA) patients and 20 healthy controls (HC) were included. Systemic disease activity in SjS was assessed with the ESSDAI score. Lymphocyte subpopulations were studied with flow cytometry. Corneal confocal microscopy and ImageJ software were used to characterize corneal sub-basal nerve plexus in terms of nerve density (CNFD), length (CNFL) and tortuosity (CNFT). Conventional dry eye tests were also performed. Results: CNFL and CNFD were lower in SjS, Sicca and RA groups, compared to HC (p < 0.001 for both SjS and Sicca); CNFL p = 0.005, CNFD p = 0.018 in RA). CNFT was higher in SjS, followed by Sicca, RA and HC. A negative correlation was found between ESSDAI score and CNFL (r=-0.735, p = 0.012). CNFL correlated negatively with IL21+ CD8+ T cells (r=-0.279, p = 0.039) and a positively with total memory (r = 0.299, p = 0.027), unswitched memory (r = 0.281, p = 0.038) and CD24Hi CD27+ (r = 0.278, p = 0.040) B cells. CNFD showed a tendency to significance in its negative correlation with ESSDAI (r=-0.592, p = 0.071) and in its positive correlation with switched memory B cells (r = 0.644, p = 0.068). Conclusions: This is the first study aiming to correlate ocular findings with lymphocyte subsets in SjS. The associations founded between CNFL and CNFD and disease activity, IL21+ follicular T cells and some B-cell subsets suggest that corneal nerve damage may parallel systemic disease activity and inflammatory cells' dynamics. |
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Corneal Sub‐Basal Nerve Plexus Assessment and its Association with Phenotypic Features and Lymphocyte Subsets in Sjögren's SyndromeEULAR Sjögren’s Syndrome Disease Activity IndexSjögren’s SyndromeCorneal confocal microscopyCorneal sub-basal nerve plexusDry eyeFlow cytometryLymphocyte subsetsHSAC OFTPurpose: To assess and compare corneal sub-basal nerve plexus morphology with circulating lymphocyte subsets, immunologic status and disease activity in Sjögren syndrome (SjS) patients. Methods: Fifty-five SjS patients, 63 Sicca patients (not fulfilling SjS criteria), 18 rheumatoid arthritis (RA) patients and 20 healthy controls (HC) were included. Systemic disease activity in SjS was assessed with the ESSDAI score. Lymphocyte subpopulations were studied with flow cytometry. Corneal confocal microscopy and ImageJ software were used to characterize corneal sub-basal nerve plexus in terms of nerve density (CNFD), length (CNFL) and tortuosity (CNFT). Conventional dry eye tests were also performed. Results: CNFL and CNFD were lower in SjS, Sicca and RA groups, compared to HC (p < 0.001 for both SjS and Sicca); CNFL p = 0.005, CNFD p = 0.018 in RA). CNFT was higher in SjS, followed by Sicca, RA and HC. A negative correlation was found between ESSDAI score and CNFL (r=-0.735, p = 0.012). CNFL correlated negatively with IL21+ CD8+ T cells (r=-0.279, p = 0.039) and a positively with total memory (r = 0.299, p = 0.027), unswitched memory (r = 0.281, p = 0.038) and CD24Hi CD27+ (r = 0.278, p = 0.040) B cells. CNFD showed a tendency to significance in its negative correlation with ESSDAI (r=-0.592, p = 0.071) and in its positive correlation with switched memory B cells (r = 0.644, p = 0.068). Conclusions: This is the first study aiming to correlate ocular findings with lymphocyte subsets in SjS. The associations founded between CNFL and CNFD and disease activity, IL21+ follicular T cells and some B-cell subsets suggest that corneal nerve damage may parallel systemic disease activity and inflammatory cells' dynamics.WileyRepositório do Centro Hospitalar Universitário de Lisboa Central, EPEBarcelos, FHipólito‐Fernandes, DMartins, CÂngelo‐Dias, MCardigos, JMonteiro, RAlves, NVaz‐Patto, Jda Cunha‐Branco, JBorrego, LMl2022-11-17T14:34:35Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.17/4283engActa Ophthalmol . 2021 Dec;99(8):e1315-e132510.1111/aos.14811info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-10T09:46:08Zoai:repositorio.chlc.min-saude.pt:10400.17/4283Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:21:36.661123Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Corneal Sub‐Basal Nerve Plexus Assessment and its Association with Phenotypic Features and Lymphocyte Subsets in Sjögren's Syndrome |
title |
Corneal Sub‐Basal Nerve Plexus Assessment and its Association with Phenotypic Features and Lymphocyte Subsets in Sjögren's Syndrome |
spellingShingle |
Corneal Sub‐Basal Nerve Plexus Assessment and its Association with Phenotypic Features and Lymphocyte Subsets in Sjögren's Syndrome Barcelos, F EULAR Sjögren’s Syndrome Disease Activity Index Sjögren’s Syndrome Corneal confocal microscopy Corneal sub-basal nerve plexus Dry eye Flow cytometry Lymphocyte subsets HSAC OFT |
title_short |
Corneal Sub‐Basal Nerve Plexus Assessment and its Association with Phenotypic Features and Lymphocyte Subsets in Sjögren's Syndrome |
title_full |
Corneal Sub‐Basal Nerve Plexus Assessment and its Association with Phenotypic Features and Lymphocyte Subsets in Sjögren's Syndrome |
title_fullStr |
Corneal Sub‐Basal Nerve Plexus Assessment and its Association with Phenotypic Features and Lymphocyte Subsets in Sjögren's Syndrome |
title_full_unstemmed |
Corneal Sub‐Basal Nerve Plexus Assessment and its Association with Phenotypic Features and Lymphocyte Subsets in Sjögren's Syndrome |
title_sort |
Corneal Sub‐Basal Nerve Plexus Assessment and its Association with Phenotypic Features and Lymphocyte Subsets in Sjögren's Syndrome |
author |
Barcelos, F |
author_facet |
Barcelos, F Hipólito‐Fernandes, D Martins, C Ângelo‐Dias, M Cardigos, J Monteiro, R Alves, N Vaz‐Patto, J da Cunha‐Branco, J Borrego, LMl |
author_role |
author |
author2 |
Hipólito‐Fernandes, D Martins, C Ângelo‐Dias, M Cardigos, J Monteiro, R Alves, N Vaz‐Patto, J da Cunha‐Branco, J Borrego, LMl |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório do Centro Hospitalar Universitário de Lisboa Central, EPE |
dc.contributor.author.fl_str_mv |
Barcelos, F Hipólito‐Fernandes, D Martins, C Ângelo‐Dias, M Cardigos, J Monteiro, R Alves, N Vaz‐Patto, J da Cunha‐Branco, J Borrego, LMl |
dc.subject.por.fl_str_mv |
EULAR Sjögren’s Syndrome Disease Activity Index Sjögren’s Syndrome Corneal confocal microscopy Corneal sub-basal nerve plexus Dry eye Flow cytometry Lymphocyte subsets HSAC OFT |
topic |
EULAR Sjögren’s Syndrome Disease Activity Index Sjögren’s Syndrome Corneal confocal microscopy Corneal sub-basal nerve plexus Dry eye Flow cytometry Lymphocyte subsets HSAC OFT |
description |
Purpose: To assess and compare corneal sub-basal nerve plexus morphology with circulating lymphocyte subsets, immunologic status and disease activity in Sjögren syndrome (SjS) patients. Methods: Fifty-five SjS patients, 63 Sicca patients (not fulfilling SjS criteria), 18 rheumatoid arthritis (RA) patients and 20 healthy controls (HC) were included. Systemic disease activity in SjS was assessed with the ESSDAI score. Lymphocyte subpopulations were studied with flow cytometry. Corneal confocal microscopy and ImageJ software were used to characterize corneal sub-basal nerve plexus in terms of nerve density (CNFD), length (CNFL) and tortuosity (CNFT). Conventional dry eye tests were also performed. Results: CNFL and CNFD were lower in SjS, Sicca and RA groups, compared to HC (p < 0.001 for both SjS and Sicca); CNFL p = 0.005, CNFD p = 0.018 in RA). CNFT was higher in SjS, followed by Sicca, RA and HC. A negative correlation was found between ESSDAI score and CNFL (r=-0.735, p = 0.012). CNFL correlated negatively with IL21+ CD8+ T cells (r=-0.279, p = 0.039) and a positively with total memory (r = 0.299, p = 0.027), unswitched memory (r = 0.281, p = 0.038) and CD24Hi CD27+ (r = 0.278, p = 0.040) B cells. CNFD showed a tendency to significance in its negative correlation with ESSDAI (r=-0.592, p = 0.071) and in its positive correlation with switched memory B cells (r = 0.644, p = 0.068). Conclusions: This is the first study aiming to correlate ocular findings with lymphocyte subsets in SjS. The associations founded between CNFL and CNFD and disease activity, IL21+ follicular T cells and some B-cell subsets suggest that corneal nerve damage may parallel systemic disease activity and inflammatory cells' dynamics. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z 2022-11-17T14:34:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.17/4283 |
url |
http://hdl.handle.net/10400.17/4283 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Ophthalmol . 2021 Dec;99(8):e1315-e1325 10.1111/aos.14811 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131310966439936 |