Cell adhesion and proliferation onto chitosan-based membranes treated by plasma surface modification
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/12985 |
Resumo: | Surface properties play a vita role in the functioning of a biomaterial. Cellular adherence and growth onto biomaterials can be enhanced in biomaterial modifications of their surface. In this work, the cell behaviour on chitosan membranes modified by argon and nitron-plasma treatments was investigated. Characterization of the membranes was performed using atomic force microscopy, contact angle measurements, and X-ray photoelectron spectroscopy. Cytotoxicity assessment and direct contact assay were carried out for untreated and treated chitosan membranes using L929 fibroblast-like cells. Cell morphology and cell viability were assessed to evaluate the cell attachment and proliferation. Changes in terms of roughness, surface chemistry, and hydrophilicity/hydrophobic balance of chitosan-modified membranes were observed. Regarding cell studies, the findings revealed that the extracts of all membranes do not induce cytotoxicity effects. Moreover, the in vitro assays evidenced an improvement of the L929 adhesion, and attachment when compared to untreated chitosan membranes. overall, the data obtained clearly demonstrated that plasma treatments constitute an effective way of improving the biocompatibility of chitosan membranes towards to their use in biomedical applications. |
id |
RCAP_7fb2fbb92b010aa0c1b0dd7f1547f768 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/12985 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Cell adhesion and proliferation onto chitosan-based membranes treated by plasma surface modificationChitosan membranePlasma treatmentCell behaviourSurface propertiesBiomaterialcell behaviorScience & TechnologySurface properties play a vita role in the functioning of a biomaterial. Cellular adherence and growth onto biomaterials can be enhanced in biomaterial modifications of their surface. In this work, the cell behaviour on chitosan membranes modified by argon and nitron-plasma treatments was investigated. Characterization of the membranes was performed using atomic force microscopy, contact angle measurements, and X-ray photoelectron spectroscopy. Cytotoxicity assessment and direct contact assay were carried out for untreated and treated chitosan membranes using L929 fibroblast-like cells. Cell morphology and cell viability were assessed to evaluate the cell attachment and proliferation. Changes in terms of roughness, surface chemistry, and hydrophilicity/hydrophobic balance of chitosan-modified membranes were observed. Regarding cell studies, the findings revealed that the extracts of all membranes do not induce cytotoxicity effects. Moreover, the in vitro assays evidenced an improvement of the L929 adhesion, and attachment when compared to untreated chitosan membranes. overall, the data obtained clearly demonstrated that plasma treatments constitute an effective way of improving the biocompatibility of chitosan membranes towards to their use in biomedical applications.S. M. Luna acknowledges the support of the Programme Alssan - The European Union Programme of High Level Scholarships for Latin America (scholarship No E04M041362CO) and Silva SS acknowledge the support of the Portuguese Foundation for Science and Technology, (SFRH/BPD/45307/2008). This work was also partially supported by the European Union-funded - STREP project HIPPOCRATES (NMP3-CT-2003-505758) and was carried out under the scope of European NoE EXPERTISSUES (NMP3-CT-2004-500283).SAGEUniversidade do MinhoLuna, Sandra M.Silva, Simone SantosGomes, Manuela E.Mano, J. F.Reis, R. L.2011-072011-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/12985eng0885-328210.1177/088532821036292420511386http://jba.sagepub.com/content/26/1/101.full.pdf+htmlinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:59:25Zoai:repositorium.sdum.uminho.pt:1822/12985Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:49:12.020145Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Cell adhesion and proliferation onto chitosan-based membranes treated by plasma surface modification |
title |
Cell adhesion and proliferation onto chitosan-based membranes treated by plasma surface modification |
spellingShingle |
Cell adhesion and proliferation onto chitosan-based membranes treated by plasma surface modification Luna, Sandra M. Chitosan membrane Plasma treatment Cell behaviour Surface properties Biomaterial cell behavior Science & Technology |
title_short |
Cell adhesion and proliferation onto chitosan-based membranes treated by plasma surface modification |
title_full |
Cell adhesion and proliferation onto chitosan-based membranes treated by plasma surface modification |
title_fullStr |
Cell adhesion and proliferation onto chitosan-based membranes treated by plasma surface modification |
title_full_unstemmed |
Cell adhesion and proliferation onto chitosan-based membranes treated by plasma surface modification |
title_sort |
Cell adhesion and proliferation onto chitosan-based membranes treated by plasma surface modification |
author |
Luna, Sandra M. |
author_facet |
Luna, Sandra M. Silva, Simone Santos Gomes, Manuela E. Mano, J. F. Reis, R. L. |
author_role |
author |
author2 |
Silva, Simone Santos Gomes, Manuela E. Mano, J. F. Reis, R. L. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Luna, Sandra M. Silva, Simone Santos Gomes, Manuela E. Mano, J. F. Reis, R. L. |
dc.subject.por.fl_str_mv |
Chitosan membrane Plasma treatment Cell behaviour Surface properties Biomaterial cell behavior Science & Technology |
topic |
Chitosan membrane Plasma treatment Cell behaviour Surface properties Biomaterial cell behavior Science & Technology |
description |
Surface properties play a vita role in the functioning of a biomaterial. Cellular adherence and growth onto biomaterials can be enhanced in biomaterial modifications of their surface. In this work, the cell behaviour on chitosan membranes modified by argon and nitron-plasma treatments was investigated. Characterization of the membranes was performed using atomic force microscopy, contact angle measurements, and X-ray photoelectron spectroscopy. Cytotoxicity assessment and direct contact assay were carried out for untreated and treated chitosan membranes using L929 fibroblast-like cells. Cell morphology and cell viability were assessed to evaluate the cell attachment and proliferation. Changes in terms of roughness, surface chemistry, and hydrophilicity/hydrophobic balance of chitosan-modified membranes were observed. Regarding cell studies, the findings revealed that the extracts of all membranes do not induce cytotoxicity effects. Moreover, the in vitro assays evidenced an improvement of the L929 adhesion, and attachment when compared to untreated chitosan membranes. overall, the data obtained clearly demonstrated that plasma treatments constitute an effective way of improving the biocompatibility of chitosan membranes towards to their use in biomedical applications. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-07 2011-07-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/12985 |
url |
http://hdl.handle.net/1822/12985 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0885-3282 10.1177/0885328210362924 20511386 http://jba.sagepub.com/content/26/1/101.full.pdf+html |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
SAGE |
publisher.none.fl_str_mv |
SAGE |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132256083640320 |