Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the layer-by-layer technique
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/1114 |
Resumo: | Tissue engineering is an important branch of regenerative medicine that uses cells, materials (scaffolds), and suitable biochemical and physicochemical factors to improve or replace specific biological functions. In particular, the control of cell behavior (namely, of cell adhesion, proliferation and differentiation) is a key aspect for the design of successful therapeutical approaches. In this study, poly(lactic-co-glycolic acid) (PLGA) fiber mats were prepared using the electrospinning technology (the fiber diameters were in the micrometer range). Furthermore, the electrospun fiber mats thus formed were functionalized using the layer-by- layer (LbL) technique with chitosan and alginate (natural and biodegradable polyelectrolytes having opposite charges) as a mean for the immobilization of pDNA/dendrimer complexes. The polyelectrolyte multilayer deposition was confirmed by fluorescence spectroscopy using fluorescent-labeled polyelectrolytes. The electrospun fiber mats coated with chitosan and alginate were successfully loaded with complexes of pDNA and poly(amidoamine) (PAMAM) dendrimers (generation 5) and were able of releasing them in a controlled manner along time. In addition, these mats supported the adhesion and proliferation of NIH 3T3 cells and of human mesenchymal stem cells (hMSCs) in their surface. Transfection experiments using a pDNA encoding for luciferase showed the ability of the electrospun fiber mats to efficiently serve as gene delivery systems. When a pDNA encoding for bone morphogenetic protein-2 (BMP-2) was used, the osteoblastic differentiation of hMSCs cultured on the surface of the mats was promoted. Taken together, the results revealed that merging the electrospinning technique with the LbL technique, can be a suitable methodology for the creation of biological active matrices for bone tissue engineering. |
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Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the layer-by-layer techniqueElectrospinningLayer-by-layerGene deliveryHuman mesenchymal stem cellsOsteoblastic differentiationNanochemistry and Nanomaterials.Centro de Ciências Exatas e da EngenhariaDomínio/Área Científica::Engenharia e Tecnologia::Engenharia QuímicaDomínio/Área Científica::Engenharia e Tecnologia::Engenharia dos MateriaisDomínio/Área Científica::Ciências MédicasTissue engineering is an important branch of regenerative medicine that uses cells, materials (scaffolds), and suitable biochemical and physicochemical factors to improve or replace specific biological functions. In particular, the control of cell behavior (namely, of cell adhesion, proliferation and differentiation) is a key aspect for the design of successful therapeutical approaches. In this study, poly(lactic-co-glycolic acid) (PLGA) fiber mats were prepared using the electrospinning technology (the fiber diameters were in the micrometer range). Furthermore, the electrospun fiber mats thus formed were functionalized using the layer-by- layer (LbL) technique with chitosan and alginate (natural and biodegradable polyelectrolytes having opposite charges) as a mean for the immobilization of pDNA/dendrimer complexes. The polyelectrolyte multilayer deposition was confirmed by fluorescence spectroscopy using fluorescent-labeled polyelectrolytes. The electrospun fiber mats coated with chitosan and alginate were successfully loaded with complexes of pDNA and poly(amidoamine) (PAMAM) dendrimers (generation 5) and were able of releasing them in a controlled manner along time. In addition, these mats supported the adhesion and proliferation of NIH 3T3 cells and of human mesenchymal stem cells (hMSCs) in their surface. Transfection experiments using a pDNA encoding for luciferase showed the ability of the electrospun fiber mats to efficiently serve as gene delivery systems. When a pDNA encoding for bone morphogenetic protein-2 (BMP-2) was used, the osteoblastic differentiation of hMSCs cultured on the surface of the mats was promoted. Taken together, the results revealed that merging the electrospinning technique with the LbL technique, can be a suitable methodology for the creation of biological active matrices for bone tissue engineering.Tomás, Helena Maria Pires GasparXiao, ShiiDigitUMaRamalingam, Kirthiga2016-05-01T00:30:11Z2014-092014-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.13/1114enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-04-02T05:42:17Zoai:digituma.uma.pt:10400.13/1114Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:03:49.329508Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the layer-by-layer technique |
title |
Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the layer-by-layer technique |
spellingShingle |
Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the layer-by-layer technique Ramalingam, Kirthiga Electrospinning Layer-by-layer Gene delivery Human mesenchymal stem cells Osteoblastic differentiation Nanochemistry and Nanomaterials . Centro de Ciências Exatas e da Engenharia Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química Domínio/Área Científica::Engenharia e Tecnologia::Engenharia dos Materiais Domínio/Área Científica::Ciências Médicas |
title_short |
Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the layer-by-layer technique |
title_full |
Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the layer-by-layer technique |
title_fullStr |
Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the layer-by-layer technique |
title_full_unstemmed |
Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the layer-by-layer technique |
title_sort |
Gene delivery using dendrimer/pDNA complexes immobilized in electrospun fibers using the layer-by-layer technique |
author |
Ramalingam, Kirthiga |
author_facet |
Ramalingam, Kirthiga |
author_role |
author |
dc.contributor.none.fl_str_mv |
Tomás, Helena Maria Pires Gaspar Xiao, Shii DigitUMa |
dc.contributor.author.fl_str_mv |
Ramalingam, Kirthiga |
dc.subject.por.fl_str_mv |
Electrospinning Layer-by-layer Gene delivery Human mesenchymal stem cells Osteoblastic differentiation Nanochemistry and Nanomaterials . Centro de Ciências Exatas e da Engenharia Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química Domínio/Área Científica::Engenharia e Tecnologia::Engenharia dos Materiais Domínio/Área Científica::Ciências Médicas |
topic |
Electrospinning Layer-by-layer Gene delivery Human mesenchymal stem cells Osteoblastic differentiation Nanochemistry and Nanomaterials . Centro de Ciências Exatas e da Engenharia Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química Domínio/Área Científica::Engenharia e Tecnologia::Engenharia dos Materiais Domínio/Área Científica::Ciências Médicas |
description |
Tissue engineering is an important branch of regenerative medicine that uses cells, materials (scaffolds), and suitable biochemical and physicochemical factors to improve or replace specific biological functions. In particular, the control of cell behavior (namely, of cell adhesion, proliferation and differentiation) is a key aspect for the design of successful therapeutical approaches. In this study, poly(lactic-co-glycolic acid) (PLGA) fiber mats were prepared using the electrospinning technology (the fiber diameters were in the micrometer range). Furthermore, the electrospun fiber mats thus formed were functionalized using the layer-by- layer (LbL) technique with chitosan and alginate (natural and biodegradable polyelectrolytes having opposite charges) as a mean for the immobilization of pDNA/dendrimer complexes. The polyelectrolyte multilayer deposition was confirmed by fluorescence spectroscopy using fluorescent-labeled polyelectrolytes. The electrospun fiber mats coated with chitosan and alginate were successfully loaded with complexes of pDNA and poly(amidoamine) (PAMAM) dendrimers (generation 5) and were able of releasing them in a controlled manner along time. In addition, these mats supported the adhesion and proliferation of NIH 3T3 cells and of human mesenchymal stem cells (hMSCs) in their surface. Transfection experiments using a pDNA encoding for luciferase showed the ability of the electrospun fiber mats to efficiently serve as gene delivery systems. When a pDNA encoding for bone morphogenetic protein-2 (BMP-2) was used, the osteoblastic differentiation of hMSCs cultured on the surface of the mats was promoted. Taken together, the results revealed that merging the electrospinning technique with the LbL technique, can be a suitable methodology for the creation of biological active matrices for bone tissue engineering. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09 2014-09-01T00:00:00Z 2016-05-01T00:30:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/1114 |
url |
http://hdl.handle.net/10400.13/1114 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799129907756793856 |