Programmable living units for emulating pancreatic tumor-stroma interplay

Detalhes bibliográficos
Autor(a) principal: Monteiro, Maria V.
Data de Publicação: 2022
Outros Autores: Rocha, Marta, Gaspar, Vítor M., Mano, João F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/34739
Resumo: Bioengineering close-to-native in vitro models that emulate tumors bioarchitecture and microenvironment is highly appreciable for improving disease modeling toolboxes. Herein, pancreatic cancer living units-so termed cancer-on-a-bead models-are generated. Such user-programmable in vitro platforms exhibit biomimetic multicompartmentalization and tunable integration of cancer associated stromal elements. These stratified units can be rapidly assembled in-air, exhibit reproducible morphological features, tunable size, and recapitulate spatially resolved tumor-stroma extracellular matrix (ECM) niches. Compartmentalization of pancreatic cancer and stromal cells in well-defined ECM microenvironments stimulates the secretion of key biomolecular effectors including transforming growth factor β and Interleukin 1-β, closely emulating the signatures of human pancreatic tumors. Cancer-on-a-bead models also display increased drug resistance to chemotherapeutics when compared to their reductionistic counterparts, reinforcing the importance to differentially model ECM components inclusion and their spatial stratification as observed in vivo. Beyond providing a universal technology that enables spatial modularity in tumor-stroma elements bioengineering, a scalable, in-air fabrication of ECM-tunable 3D platforms that can be leveraged for recapitulating differential matrix composition occurring in other human neoplasias is provided here.
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spelling Programmable living units for emulating pancreatic tumor-stroma interplay3D in vitro tumor modelsPancreatic tumor microenvironmentPreclinical drug screensSuperhydrophobic surfacesBioengineering close-to-native in vitro models that emulate tumors bioarchitecture and microenvironment is highly appreciable for improving disease modeling toolboxes. Herein, pancreatic cancer living units-so termed cancer-on-a-bead models-are generated. Such user-programmable in vitro platforms exhibit biomimetic multicompartmentalization and tunable integration of cancer associated stromal elements. These stratified units can be rapidly assembled in-air, exhibit reproducible morphological features, tunable size, and recapitulate spatially resolved tumor-stroma extracellular matrix (ECM) niches. Compartmentalization of pancreatic cancer and stromal cells in well-defined ECM microenvironments stimulates the secretion of key biomolecular effectors including transforming growth factor β and Interleukin 1-β, closely emulating the signatures of human pancreatic tumors. Cancer-on-a-bead models also display increased drug resistance to chemotherapeutics when compared to their reductionistic counterparts, reinforcing the importance to differentially model ECM components inclusion and their spatial stratification as observed in vivo. Beyond providing a universal technology that enables spatial modularity in tumor-stroma elements bioengineering, a scalable, in-air fabrication of ECM-tunable 3D platforms that can be leveraged for recapitulating differential matrix composition occurring in other human neoplasias is provided here.Wiley-Blackwell2023-07-01T00:00:00Z2022-07-06T00:00:00Z2022-07-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/34739eng2192-264010.1002/adhm.202102574Monteiro, Maria V.Rocha, MartaGaspar, Vítor M.Mano, João F.info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:07:15Zoai:ria.ua.pt:10773/34739Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:06:04.043152Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Programmable living units for emulating pancreatic tumor-stroma interplay
title Programmable living units for emulating pancreatic tumor-stroma interplay
spellingShingle Programmable living units for emulating pancreatic tumor-stroma interplay
Monteiro, Maria V.
3D in vitro tumor models
Pancreatic tumor microenvironment
Preclinical drug screens
Superhydrophobic surfaces
title_short Programmable living units for emulating pancreatic tumor-stroma interplay
title_full Programmable living units for emulating pancreatic tumor-stroma interplay
title_fullStr Programmable living units for emulating pancreatic tumor-stroma interplay
title_full_unstemmed Programmable living units for emulating pancreatic tumor-stroma interplay
title_sort Programmable living units for emulating pancreatic tumor-stroma interplay
author Monteiro, Maria V.
author_facet Monteiro, Maria V.
Rocha, Marta
Gaspar, Vítor M.
Mano, João F.
author_role author
author2 Rocha, Marta
Gaspar, Vítor M.
Mano, João F.
author2_role author
author
author
dc.contributor.author.fl_str_mv Monteiro, Maria V.
Rocha, Marta
Gaspar, Vítor M.
Mano, João F.
dc.subject.por.fl_str_mv 3D in vitro tumor models
Pancreatic tumor microenvironment
Preclinical drug screens
Superhydrophobic surfaces
topic 3D in vitro tumor models
Pancreatic tumor microenvironment
Preclinical drug screens
Superhydrophobic surfaces
description Bioengineering close-to-native in vitro models that emulate tumors bioarchitecture and microenvironment is highly appreciable for improving disease modeling toolboxes. Herein, pancreatic cancer living units-so termed cancer-on-a-bead models-are generated. Such user-programmable in vitro platforms exhibit biomimetic multicompartmentalization and tunable integration of cancer associated stromal elements. These stratified units can be rapidly assembled in-air, exhibit reproducible morphological features, tunable size, and recapitulate spatially resolved tumor-stroma extracellular matrix (ECM) niches. Compartmentalization of pancreatic cancer and stromal cells in well-defined ECM microenvironments stimulates the secretion of key biomolecular effectors including transforming growth factor β and Interleukin 1-β, closely emulating the signatures of human pancreatic tumors. Cancer-on-a-bead models also display increased drug resistance to chemotherapeutics when compared to their reductionistic counterparts, reinforcing the importance to differentially model ECM components inclusion and their spatial stratification as observed in vivo. Beyond providing a universal technology that enables spatial modularity in tumor-stroma elements bioengineering, a scalable, in-air fabrication of ECM-tunable 3D platforms that can be leveraged for recapitulating differential matrix composition occurring in other human neoplasias is provided here.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-06T00:00:00Z
2022-07-06
2023-07-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/34739
url http://hdl.handle.net/10773/34739
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2192-2640
10.1002/adhm.202102574
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dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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