Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections

Detalhes bibliográficos
Autor(a) principal: Fernandes, Laura Silva
Data de Publicação: 2021
Outros Autores: Costa, Ygor Ferreira Garcia da, Bessa, Martha Eunice de, Ferreira, Adriana Lucia Pires, Corrêa, José Otávio do Amaral, Del-Vechio Vieira, Glauciemar, Sousa, Orlando Vieira de, Araújo, Ana Lúcia Santos de Matos, Castilho, Paula C., Alves, Maria Silvana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.13/4676
Resumo: Morbidity and mortality of the infected patients by multidrug-resistant bacteria have increased, emphasizing the urgency of fi ght for the discovery of new innovative antibiotics. In this sense, natural products emerge as valuable sources of bioactive compounds. Among the biodiversity, Eryngium pristis Cham. & Schltdl. (Apiaceae Lindl.) is traditionally used to treat thrush and ulcers of throat and mouth, as diuretic and emmenagogue, but scarcely known as an antimicrobial agent. With this context in mind, the goals of this study were to investigate the metabolic profi le and the antibacterial activity of ethanolic extract (EE-Ep) and hexane (HF-Ep), dichloromethane (DF-Ep), ethyl acetate (EAF-Ep) and butanol (BF-Ep) fractions from E. pristis leaves. Gas Chromatography-Mass Spectrometry (GC-MS) was performed to stablish the metabolic profi le and revealed the presence of 12 and 14 compounds in EAF-Ep and HF-Ep, respectively. β-selinene, spathulenol, globulol, 2-methoxy-4-vinylphenol, α-amyrin, β-amyrin, and lupeol derivative were some of phytochemicals identifi ed. The antibacterial activity was determined by Minimal Inhibitory Concentration (MIC) using the broth micro-dilution against eight ATCC® and fi ve methicillin-resistant Staphylococcus aureus (MRSA) clinical strains. HF-Ep was the most eff ective (MIC ≤ 5,000 μg/μL), being active against the largest part of tested Gram-positive and Gram-negative bacterial strains, including MRSA, with exception of Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 9027) and (ATCC 27853). These results suggest that E. pristis is a natural source of bioactive compounds for the search of new antibiotics which can be an interesting therapeutic approach to recover patients mainly infected by MRSA strains.
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spelling Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infectionsEryngium pristisApiaceaeAnti bacterial activityBacterial resistanceStaphy lococcus aureusMethicillin-resistant Staphy lococcus aureus.Faculdade de Ciências Exatas e da EngenhariaMorbidity and mortality of the infected patients by multidrug-resistant bacteria have increased, emphasizing the urgency of fi ght for the discovery of new innovative antibiotics. In this sense, natural products emerge as valuable sources of bioactive compounds. Among the biodiversity, Eryngium pristis Cham. & Schltdl. (Apiaceae Lindl.) is traditionally used to treat thrush and ulcers of throat and mouth, as diuretic and emmenagogue, but scarcely known as an antimicrobial agent. With this context in mind, the goals of this study were to investigate the metabolic profi le and the antibacterial activity of ethanolic extract (EE-Ep) and hexane (HF-Ep), dichloromethane (DF-Ep), ethyl acetate (EAF-Ep) and butanol (BF-Ep) fractions from E. pristis leaves. Gas Chromatography-Mass Spectrometry (GC-MS) was performed to stablish the metabolic profi le and revealed the presence of 12 and 14 compounds in EAF-Ep and HF-Ep, respectively. β-selinene, spathulenol, globulol, 2-methoxy-4-vinylphenol, α-amyrin, β-amyrin, and lupeol derivative were some of phytochemicals identifi ed. The antibacterial activity was determined by Minimal Inhibitory Concentration (MIC) using the broth micro-dilution against eight ATCC® and fi ve methicillin-resistant Staphylococcus aureus (MRSA) clinical strains. HF-Ep was the most eff ective (MIC ≤ 5,000 μg/μL), being active against the largest part of tested Gram-positive and Gram-negative bacterial strains, including MRSA, with exception of Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 9027) and (ATCC 27853). These results suggest that E. pristis is a natural source of bioactive compounds for the search of new antibiotics which can be an interesting therapeutic approach to recover patients mainly infected by MRSA strains.Heighten Science Publications IncDigitUMaFernandes, Laura SilvaCosta, Ygor Ferreira Garcia daBessa, Martha Eunice deFerreira, Adriana Lucia PiresCorrêa, José Otávio do AmaralDel-Vechio Vieira, GlauciemarSousa, Orlando Vieira deAraújo, Ana Lúcia Santos de MatosCastilho, Paula C.Alves, Maria Silvana2022-09-30T11:04:03Z2021-01-01T00:00:00Z2021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/4676engFernandes LS, da Costa YFG, de Bessa ME, Ferreira ALP, do Amaral Corrêa JO, et al. Metabolic profi ling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections. Arch Pharm Pharma Sci. 2021; 5: 020-028. DOI: 10.29328/journal.apps.100102710.29328/journal.apps.1001027info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-02T03:30:21Zoai:digituma.uma.pt:10400.13/4676Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:10:32.601813Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections
title Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections
spellingShingle Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections
Fernandes, Laura Silva
Eryngium pristis
Apiaceae
Anti bacterial activity
Bacterial resistance
Staphy lococcus aureus
Methicillin-resistant Staphy lococcus aureus
.
Faculdade de Ciências Exatas e da Engenharia
title_short Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections
title_full Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections
title_fullStr Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections
title_full_unstemmed Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections
title_sort Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections
author Fernandes, Laura Silva
author_facet Fernandes, Laura Silva
Costa, Ygor Ferreira Garcia da
Bessa, Martha Eunice de
Ferreira, Adriana Lucia Pires
Corrêa, José Otávio do Amaral
Del-Vechio Vieira, Glauciemar
Sousa, Orlando Vieira de
Araújo, Ana Lúcia Santos de Matos
Castilho, Paula C.
Alves, Maria Silvana
author_role author
author2 Costa, Ygor Ferreira Garcia da
Bessa, Martha Eunice de
Ferreira, Adriana Lucia Pires
Corrêa, José Otávio do Amaral
Del-Vechio Vieira, Glauciemar
Sousa, Orlando Vieira de
Araújo, Ana Lúcia Santos de Matos
Castilho, Paula C.
Alves, Maria Silvana
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DigitUMa
dc.contributor.author.fl_str_mv Fernandes, Laura Silva
Costa, Ygor Ferreira Garcia da
Bessa, Martha Eunice de
Ferreira, Adriana Lucia Pires
Corrêa, José Otávio do Amaral
Del-Vechio Vieira, Glauciemar
Sousa, Orlando Vieira de
Araújo, Ana Lúcia Santos de Matos
Castilho, Paula C.
Alves, Maria Silvana
dc.subject.por.fl_str_mv Eryngium pristis
Apiaceae
Anti bacterial activity
Bacterial resistance
Staphy lococcus aureus
Methicillin-resistant Staphy lococcus aureus
.
Faculdade de Ciências Exatas e da Engenharia
topic Eryngium pristis
Apiaceae
Anti bacterial activity
Bacterial resistance
Staphy lococcus aureus
Methicillin-resistant Staphy lococcus aureus
.
Faculdade de Ciências Exatas e da Engenharia
description Morbidity and mortality of the infected patients by multidrug-resistant bacteria have increased, emphasizing the urgency of fi ght for the discovery of new innovative antibiotics. In this sense, natural products emerge as valuable sources of bioactive compounds. Among the biodiversity, Eryngium pristis Cham. & Schltdl. (Apiaceae Lindl.) is traditionally used to treat thrush and ulcers of throat and mouth, as diuretic and emmenagogue, but scarcely known as an antimicrobial agent. With this context in mind, the goals of this study were to investigate the metabolic profi le and the antibacterial activity of ethanolic extract (EE-Ep) and hexane (HF-Ep), dichloromethane (DF-Ep), ethyl acetate (EAF-Ep) and butanol (BF-Ep) fractions from E. pristis leaves. Gas Chromatography-Mass Spectrometry (GC-MS) was performed to stablish the metabolic profi le and revealed the presence of 12 and 14 compounds in EAF-Ep and HF-Ep, respectively. β-selinene, spathulenol, globulol, 2-methoxy-4-vinylphenol, α-amyrin, β-amyrin, and lupeol derivative were some of phytochemicals identifi ed. The antibacterial activity was determined by Minimal Inhibitory Concentration (MIC) using the broth micro-dilution against eight ATCC® and fi ve methicillin-resistant Staphylococcus aureus (MRSA) clinical strains. HF-Ep was the most eff ective (MIC ≤ 5,000 μg/μL), being active against the largest part of tested Gram-positive and Gram-negative bacterial strains, including MRSA, with exception of Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 9027) and (ATCC 27853). These results suggest that E. pristis is a natural source of bioactive compounds for the search of new antibiotics which can be an interesting therapeutic approach to recover patients mainly infected by MRSA strains.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01T00:00:00Z
2021-01-01T00:00:00Z
2022-09-30T11:04:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.13/4676
url http://hdl.handle.net/10400.13/4676
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fernandes LS, da Costa YFG, de Bessa ME, Ferreira ALP, do Amaral Corrêa JO, et al. Metabolic profi ling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections. Arch Pharm Pharma Sci. 2021; 5: 020-028. DOI: 10.29328/journal.apps.1001027
10.29328/journal.apps.1001027
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Heighten Science Publications Inc
publisher.none.fl_str_mv Heighten Science Publications Inc
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