Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/4676 |
Resumo: | Morbidity and mortality of the infected patients by multidrug-resistant bacteria have increased, emphasizing the urgency of fi ght for the discovery of new innovative antibiotics. In this sense, natural products emerge as valuable sources of bioactive compounds. Among the biodiversity, Eryngium pristis Cham. & Schltdl. (Apiaceae Lindl.) is traditionally used to treat thrush and ulcers of throat and mouth, as diuretic and emmenagogue, but scarcely known as an antimicrobial agent. With this context in mind, the goals of this study were to investigate the metabolic profi le and the antibacterial activity of ethanolic extract (EE-Ep) and hexane (HF-Ep), dichloromethane (DF-Ep), ethyl acetate (EAF-Ep) and butanol (BF-Ep) fractions from E. pristis leaves. Gas Chromatography-Mass Spectrometry (GC-MS) was performed to stablish the metabolic profi le and revealed the presence of 12 and 14 compounds in EAF-Ep and HF-Ep, respectively. β-selinene, spathulenol, globulol, 2-methoxy-4-vinylphenol, α-amyrin, β-amyrin, and lupeol derivative were some of phytochemicals identifi ed. The antibacterial activity was determined by Minimal Inhibitory Concentration (MIC) using the broth micro-dilution against eight ATCC® and fi ve methicillin-resistant Staphylococcus aureus (MRSA) clinical strains. HF-Ep was the most eff ective (MIC ≤ 5,000 μg/μL), being active against the largest part of tested Gram-positive and Gram-negative bacterial strains, including MRSA, with exception of Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 9027) and (ATCC 27853). These results suggest that E. pristis is a natural source of bioactive compounds for the search of new antibiotics which can be an interesting therapeutic approach to recover patients mainly infected by MRSA strains. |
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Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infectionsEryngium pristisApiaceaeAnti bacterial activityBacterial resistanceStaphy lococcus aureusMethicillin-resistant Staphy lococcus aureus.Faculdade de Ciências Exatas e da EngenhariaMorbidity and mortality of the infected patients by multidrug-resistant bacteria have increased, emphasizing the urgency of fi ght for the discovery of new innovative antibiotics. In this sense, natural products emerge as valuable sources of bioactive compounds. Among the biodiversity, Eryngium pristis Cham. & Schltdl. (Apiaceae Lindl.) is traditionally used to treat thrush and ulcers of throat and mouth, as diuretic and emmenagogue, but scarcely known as an antimicrobial agent. With this context in mind, the goals of this study were to investigate the metabolic profi le and the antibacterial activity of ethanolic extract (EE-Ep) and hexane (HF-Ep), dichloromethane (DF-Ep), ethyl acetate (EAF-Ep) and butanol (BF-Ep) fractions from E. pristis leaves. Gas Chromatography-Mass Spectrometry (GC-MS) was performed to stablish the metabolic profi le and revealed the presence of 12 and 14 compounds in EAF-Ep and HF-Ep, respectively. β-selinene, spathulenol, globulol, 2-methoxy-4-vinylphenol, α-amyrin, β-amyrin, and lupeol derivative were some of phytochemicals identifi ed. The antibacterial activity was determined by Minimal Inhibitory Concentration (MIC) using the broth micro-dilution against eight ATCC® and fi ve methicillin-resistant Staphylococcus aureus (MRSA) clinical strains. HF-Ep was the most eff ective (MIC ≤ 5,000 μg/μL), being active against the largest part of tested Gram-positive and Gram-negative bacterial strains, including MRSA, with exception of Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 9027) and (ATCC 27853). These results suggest that E. pristis is a natural source of bioactive compounds for the search of new antibiotics which can be an interesting therapeutic approach to recover patients mainly infected by MRSA strains.Heighten Science Publications IncDigitUMaFernandes, Laura SilvaCosta, Ygor Ferreira Garcia daBessa, Martha Eunice deFerreira, Adriana Lucia PiresCorrêa, José Otávio do AmaralDel-Vechio Vieira, GlauciemarSousa, Orlando Vieira deAraújo, Ana Lúcia Santos de MatosCastilho, Paula C.Alves, Maria Silvana2022-09-30T11:04:03Z2021-01-01T00:00:00Z2021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/4676engFernandes LS, da Costa YFG, de Bessa ME, Ferreira ALP, do Amaral Corrêa JO, et al. Metabolic profi ling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections. Arch Pharm Pharma Sci. 2021; 5: 020-028. DOI: 10.29328/journal.apps.100102710.29328/journal.apps.1001027info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-02T03:30:21Zoai:digituma.uma.pt:10400.13/4676Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:10:32.601813Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections |
title |
Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections |
spellingShingle |
Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections Fernandes, Laura Silva Eryngium pristis Apiaceae Anti bacterial activity Bacterial resistance Staphy lococcus aureus Methicillin-resistant Staphy lococcus aureus . Faculdade de Ciências Exatas e da Engenharia |
title_short |
Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections |
title_full |
Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections |
title_fullStr |
Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections |
title_full_unstemmed |
Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections |
title_sort |
Metabolic profiling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections |
author |
Fernandes, Laura Silva |
author_facet |
Fernandes, Laura Silva Costa, Ygor Ferreira Garcia da Bessa, Martha Eunice de Ferreira, Adriana Lucia Pires Corrêa, José Otávio do Amaral Del-Vechio Vieira, Glauciemar Sousa, Orlando Vieira de Araújo, Ana Lúcia Santos de Matos Castilho, Paula C. Alves, Maria Silvana |
author_role |
author |
author2 |
Costa, Ygor Ferreira Garcia da Bessa, Martha Eunice de Ferreira, Adriana Lucia Pires Corrêa, José Otávio do Amaral Del-Vechio Vieira, Glauciemar Sousa, Orlando Vieira de Araújo, Ana Lúcia Santos de Matos Castilho, Paula C. Alves, Maria Silvana |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Fernandes, Laura Silva Costa, Ygor Ferreira Garcia da Bessa, Martha Eunice de Ferreira, Adriana Lucia Pires Corrêa, José Otávio do Amaral Del-Vechio Vieira, Glauciemar Sousa, Orlando Vieira de Araújo, Ana Lúcia Santos de Matos Castilho, Paula C. Alves, Maria Silvana |
dc.subject.por.fl_str_mv |
Eryngium pristis Apiaceae Anti bacterial activity Bacterial resistance Staphy lococcus aureus Methicillin-resistant Staphy lococcus aureus . Faculdade de Ciências Exatas e da Engenharia |
topic |
Eryngium pristis Apiaceae Anti bacterial activity Bacterial resistance Staphy lococcus aureus Methicillin-resistant Staphy lococcus aureus . Faculdade de Ciências Exatas e da Engenharia |
description |
Morbidity and mortality of the infected patients by multidrug-resistant bacteria have increased, emphasizing the urgency of fi ght for the discovery of new innovative antibiotics. In this sense, natural products emerge as valuable sources of bioactive compounds. Among the biodiversity, Eryngium pristis Cham. & Schltdl. (Apiaceae Lindl.) is traditionally used to treat thrush and ulcers of throat and mouth, as diuretic and emmenagogue, but scarcely known as an antimicrobial agent. With this context in mind, the goals of this study were to investigate the metabolic profi le and the antibacterial activity of ethanolic extract (EE-Ep) and hexane (HF-Ep), dichloromethane (DF-Ep), ethyl acetate (EAF-Ep) and butanol (BF-Ep) fractions from E. pristis leaves. Gas Chromatography-Mass Spectrometry (GC-MS) was performed to stablish the metabolic profi le and revealed the presence of 12 and 14 compounds in EAF-Ep and HF-Ep, respectively. β-selinene, spathulenol, globulol, 2-methoxy-4-vinylphenol, α-amyrin, β-amyrin, and lupeol derivative were some of phytochemicals identifi ed. The antibacterial activity was determined by Minimal Inhibitory Concentration (MIC) using the broth micro-dilution against eight ATCC® and fi ve methicillin-resistant Staphylococcus aureus (MRSA) clinical strains. HF-Ep was the most eff ective (MIC ≤ 5,000 μg/μL), being active against the largest part of tested Gram-positive and Gram-negative bacterial strains, including MRSA, with exception of Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 9027) and (ATCC 27853). These results suggest that E. pristis is a natural source of bioactive compounds for the search of new antibiotics which can be an interesting therapeutic approach to recover patients mainly infected by MRSA strains. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01T00:00:00Z 2021-01-01T00:00:00Z 2022-09-30T11:04:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/4676 |
url |
http://hdl.handle.net/10400.13/4676 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fernandes LS, da Costa YFG, de Bessa ME, Ferreira ALP, do Amaral Corrêa JO, et al. Metabolic profi ling and antibacterial activity of Eryngium pristis Cham. & Schltdl. - prospecting for its use in the treatment of bacterial infections. Arch Pharm Pharma Sci. 2021; 5: 020-028. DOI: 10.29328/journal.apps.1001027 10.29328/journal.apps.1001027 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Heighten Science Publications Inc |
publisher.none.fl_str_mv |
Heighten Science Publications Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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