Symbionts commonly provide broad spectrum resistance to viruses in insects: a comparative analysis of Wolbachia strains
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/341 |
Resumo: | In the last decade, bacterial symbionts have been shown to play an important role in protecting hosts against pathogens. Wolbachia, a widespread symbiont in arthropods, can protect Drosophila and mosquito species against viral infections. We have investigated antiviral protection in 19 Wolbachia strains originating from 16 Drosophila species after transfer into the same genotype of Drosophila simulans. We found that approximately half of the strains protected against two RNA viruses. Given that 40% of terrestrial arthropod species are estimated to harbour Wolbachia, as many as a fifth of all arthropods species may benefit from Wolbachia-mediated protection. The level of protection against two distantly related RNA viruses--DCV and FHV--was strongly genetically correlated, which suggests that there is a single mechanism of protection with broad specificity. Furthermore, Wolbachia is making flies resistant to viruses, as increases in survival can be largely explained by reductions in viral titer. Variation in the level of antiviral protection provided by different Wolbachia strains is strongly genetically correlated to the density of the bacteria strains in host tissues. We found no support for two previously proposed mechanisms of Wolbachia-mediated protection--activation of the immune system and upregulation of the methyltransferase Dnmt2. The large variation in Wolbachia's antiviral properties highlights the need to carefully select Wolbachia strains introduced into mosquito populations to prevent the transmission of arboviruses. |
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Symbionts commonly provide broad spectrum resistance to viruses in insects: a comparative analysis of Wolbachia strainsWolbachiaDrosophilaDengue virusMicrobial GeneticsIn the last decade, bacterial symbionts have been shown to play an important role in protecting hosts against pathogens. Wolbachia, a widespread symbiont in arthropods, can protect Drosophila and mosquito species against viral infections. We have investigated antiviral protection in 19 Wolbachia strains originating from 16 Drosophila species after transfer into the same genotype of Drosophila simulans. We found that approximately half of the strains protected against two RNA viruses. Given that 40% of terrestrial arthropod species are estimated to harbour Wolbachia, as many as a fifth of all arthropods species may benefit from Wolbachia-mediated protection. The level of protection against two distantly related RNA viruses--DCV and FHV--was strongly genetically correlated, which suggests that there is a single mechanism of protection with broad specificity. Furthermore, Wolbachia is making flies resistant to viruses, as increases in survival can be largely explained by reductions in viral titer. Variation in the level of antiviral protection provided by different Wolbachia strains is strongly genetically correlated to the density of the bacteria strains in host tissues. We found no support for two previously proposed mechanisms of Wolbachia-mediated protection--activation of the immune system and upregulation of the methyltransferase Dnmt2. The large variation in Wolbachia's antiviral properties highlights the need to carefully select Wolbachia strains introduced into mosquito populations to prevent the transmission of arboviruses.Wellcome Trust grant WT094664MA, Royal Society Research Fellowship.PLOSARCAMartinez, JulienLongdon, BenBauer, SimoneChan, Yuk-SangMiller, Wolfgang JBourtzis, KostasTeixeira, LuisJiggins, Francis M2015-10-02T15:05:32Z2014-09-182014-09-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/341engMartinez J, Longdon B, Bauer S, Chan Y-S, Miller WJ, Bourtzis K, et al. (2014) Symbionts Commonly Provide Broad Spectrum Resistance to Viruses in Insects: A Comparative Analysis of Wolbachia Strains. PLoS Pathog 10(9): e1004369. doi:10.1371/journal.ppat.100436910.1371/journal.ppat.1004369info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:34:44Zoai:arca.igc.gulbenkian.pt:10400.7/341Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:39.237195Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Symbionts commonly provide broad spectrum resistance to viruses in insects: a comparative analysis of Wolbachia strains |
title |
Symbionts commonly provide broad spectrum resistance to viruses in insects: a comparative analysis of Wolbachia strains |
spellingShingle |
Symbionts commonly provide broad spectrum resistance to viruses in insects: a comparative analysis of Wolbachia strains Martinez, Julien Wolbachia Drosophila Dengue virus Microbial Genetics |
title_short |
Symbionts commonly provide broad spectrum resistance to viruses in insects: a comparative analysis of Wolbachia strains |
title_full |
Symbionts commonly provide broad spectrum resistance to viruses in insects: a comparative analysis of Wolbachia strains |
title_fullStr |
Symbionts commonly provide broad spectrum resistance to viruses in insects: a comparative analysis of Wolbachia strains |
title_full_unstemmed |
Symbionts commonly provide broad spectrum resistance to viruses in insects: a comparative analysis of Wolbachia strains |
title_sort |
Symbionts commonly provide broad spectrum resistance to viruses in insects: a comparative analysis of Wolbachia strains |
author |
Martinez, Julien |
author_facet |
Martinez, Julien Longdon, Ben Bauer, Simone Chan, Yuk-Sang Miller, Wolfgang J Bourtzis, Kostas Teixeira, Luis Jiggins, Francis M |
author_role |
author |
author2 |
Longdon, Ben Bauer, Simone Chan, Yuk-Sang Miller, Wolfgang J Bourtzis, Kostas Teixeira, Luis Jiggins, Francis M |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Martinez, Julien Longdon, Ben Bauer, Simone Chan, Yuk-Sang Miller, Wolfgang J Bourtzis, Kostas Teixeira, Luis Jiggins, Francis M |
dc.subject.por.fl_str_mv |
Wolbachia Drosophila Dengue virus Microbial Genetics |
topic |
Wolbachia Drosophila Dengue virus Microbial Genetics |
description |
In the last decade, bacterial symbionts have been shown to play an important role in protecting hosts against pathogens. Wolbachia, a widespread symbiont in arthropods, can protect Drosophila and mosquito species against viral infections. We have investigated antiviral protection in 19 Wolbachia strains originating from 16 Drosophila species after transfer into the same genotype of Drosophila simulans. We found that approximately half of the strains protected against two RNA viruses. Given that 40% of terrestrial arthropod species are estimated to harbour Wolbachia, as many as a fifth of all arthropods species may benefit from Wolbachia-mediated protection. The level of protection against two distantly related RNA viruses--DCV and FHV--was strongly genetically correlated, which suggests that there is a single mechanism of protection with broad specificity. Furthermore, Wolbachia is making flies resistant to viruses, as increases in survival can be largely explained by reductions in viral titer. Variation in the level of antiviral protection provided by different Wolbachia strains is strongly genetically correlated to the density of the bacteria strains in host tissues. We found no support for two previously proposed mechanisms of Wolbachia-mediated protection--activation of the immune system and upregulation of the methyltransferase Dnmt2. The large variation in Wolbachia's antiviral properties highlights the need to carefully select Wolbachia strains introduced into mosquito populations to prevent the transmission of arboviruses. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-18 2014-09-18T00:00:00Z 2015-10-02T15:05:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/341 |
url |
http://hdl.handle.net/10400.7/341 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Martinez J, Longdon B, Bauer S, Chan Y-S, Miller WJ, Bourtzis K, et al. (2014) Symbionts Commonly Provide Broad Spectrum Resistance to Viruses in Insects: A Comparative Analysis of Wolbachia Strains. PLoS Pathog 10(9): e1004369. doi:10.1371/journal.ppat.1004369 10.1371/journal.ppat.1004369 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
PLOS |
publisher.none.fl_str_mv |
PLOS |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130572155518976 |