Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways

Detalhes bibliográficos
Autor(a) principal: Teixeira, António
Data de Publicação: 2020
Outros Autores: Martins, Viviana, Frusciante, Sarah, Cruz, Telmo, Noronha, Henrique, Diretto, Gianfranco, Gerós, H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/65697
Resumo: The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2020.00896/full#supplementary-material
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spelling Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathwaysVitis viniferaflavescence doreebiotic stressleaf yellowingisoprenoids biosynthesisplant pathogensScience & TechnologyThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2020.00896/full#supplementary-materialFlavescence dorée (FD), caused by the phytoplasma Candidatus Phytoplasma vitis, is a major threat to vineyard survival in different European grape-growing areas. It has been recorded in French vineyards since the mid-1950s, and rapidly spread to other countries. In Portugal, the phytoplasma was first detected in the DOC region of Vinhos Verdes in 2006, and reached the central region of the country in 2009. The infection causes strong accumulation of carbohydrates and phenolics in the mesophyll cells and a simultaneous decrease of chlorophylls, events accompanied by a down regulation of genes and proteins involved in the dark and light-dependent reactions and stabilization of the photosystem II (PSII). In the present study, to better elucidate the basis of the leaf chlorosis in infected grapevine cv. Loureiro, we studied the isoprenoid transcriptmetabolite correlation in leaves from healthy and FD-infected vines. Specifically, targeted metabolome revealed that twenty-one compounds (out of thirty-two), including chlorophylls, carotenoids, quinones and tocopherols, were reduced in response to FD-infection. Thereafter, and consistently with the biochemical data, qPCR analysis highlighted a severe FD-mediated repression in key genes involved in isoprenoid biosynthetic pathways. A more diverse set of changes, on the contrary, was observed in the case of ABA metabolism. Principal component analysis (PCA) of all identified metabolites clearly separated healthy from FD-infected vines, therefore confirming that the infection strongly alters the biosynthesis of grapevine isoprenoids; additionally, forty-four genes and metabolites were identified as the components mostly explaining the variance between healthy and infected samples. Finally, transcriptmetabolite network correlation analyses were exploited to display the main hubs of the infection process, which highlighted a strong role of VvCHLG, VvVTE and VvZEP genes and the chlorophylls intermediates aminolevulunic acid and porphobilinogen in response to FD infection. Overall, results indicated that the FD infection impairs the synthesis of isoprenoids, through the repression of key genes involved in the biosynthesis of chlorophylls, carotenoids, quinones and tocopherols.The work was supported by National Funds by FCT - Portuguese Foundation for Science and Technology, under the strategic program UIDB/04050/2020. The work was also supported by FCT and European Funds (FEDER/POCI/ COMPETE2020) through the research projects BerryPlastid (PTDC/BIAFBT/28165/2017 and POCI-01-0145-FEDER - 028165), MitiVineDrought (PTDC/BIA-FBT/30341/2017 and POCI-01- 0145-FEDER-030341) and GrapeInfectomics (PTDC/ASPHOR/28485/2017). AT was supported by a postdoctoral researcher contract/position within the project “BerryPlastid”. HN was supported by an FCT postdoctoral grant (SFRH/BPD/115518/2016). The work also benefited from the networking activities within the European Cooperation in Science and Technology Action (EUROCAROTEN CA15136).info:eu-repo/semantics/publishedVersionFrontiers MediaUniversidade do MinhoTeixeira, AntónioMartins, VivianaFrusciante, SarahCruz, TelmoNoronha, HenriqueDiretto, GianfrancoGerós, H.2020-06-172020-06-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/65697engTeixeira, António; Martins, Viviana; Frusciante, Sarah; Cruz, Telmo; Noronha, Henrique; Diretto, Gianfranco; Gerós, Hernâni, Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways. Frontiers in Plant Science, 11(896), 20201664-462X10.3389/fpls.2020.00896http://www.frontiersin.org/Plant_Physiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T07:40:35Zoai:repositorium.sdum.uminho.pt:1822/65697Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T07:40:35Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways
title Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways
spellingShingle Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways
Teixeira, António
Vitis vinifera
flavescence doree
biotic stress
leaf yellowing
isoprenoids biosynthesis
plant pathogens
Science & Technology
title_short Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways
title_full Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways
title_fullStr Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways
title_full_unstemmed Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways
title_sort Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways
author Teixeira, António
author_facet Teixeira, António
Martins, Viviana
Frusciante, Sarah
Cruz, Telmo
Noronha, Henrique
Diretto, Gianfranco
Gerós, H.
author_role author
author2 Martins, Viviana
Frusciante, Sarah
Cruz, Telmo
Noronha, Henrique
Diretto, Gianfranco
Gerós, H.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Teixeira, António
Martins, Viviana
Frusciante, Sarah
Cruz, Telmo
Noronha, Henrique
Diretto, Gianfranco
Gerós, H.
dc.subject.por.fl_str_mv Vitis vinifera
flavescence doree
biotic stress
leaf yellowing
isoprenoids biosynthesis
plant pathogens
Science & Technology
topic Vitis vinifera
flavescence doree
biotic stress
leaf yellowing
isoprenoids biosynthesis
plant pathogens
Science & Technology
description The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2020.00896/full#supplementary-material
publishDate 2020
dc.date.none.fl_str_mv 2020-06-17
2020-06-17T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/65697
url http://hdl.handle.net/1822/65697
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Teixeira, António; Martins, Viviana; Frusciante, Sarah; Cruz, Telmo; Noronha, Henrique; Diretto, Gianfranco; Gerós, Hernâni, Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways. Frontiers in Plant Science, 11(896), 2020
1664-462X
10.3389/fpls.2020.00896
http://www.frontiersin.org/Plant_Physiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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