Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/65697 |
Resumo: | The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2020.00896/full#supplementary-material |
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Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathwaysVitis viniferaflavescence doreebiotic stressleaf yellowingisoprenoids biosynthesisplant pathogensScience & TechnologyThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2020.00896/full#supplementary-materialFlavescence dorée (FD), caused by the phytoplasma Candidatus Phytoplasma vitis, is a major threat to vineyard survival in different European grape-growing areas. It has been recorded in French vineyards since the mid-1950s, and rapidly spread to other countries. In Portugal, the phytoplasma was first detected in the DOC region of Vinhos Verdes in 2006, and reached the central region of the country in 2009. The infection causes strong accumulation of carbohydrates and phenolics in the mesophyll cells and a simultaneous decrease of chlorophylls, events accompanied by a down regulation of genes and proteins involved in the dark and light-dependent reactions and stabilization of the photosystem II (PSII). In the present study, to better elucidate the basis of the leaf chlorosis in infected grapevine cv. Loureiro, we studied the isoprenoid transcriptmetabolite correlation in leaves from healthy and FD-infected vines. Specifically, targeted metabolome revealed that twenty-one compounds (out of thirty-two), including chlorophylls, carotenoids, quinones and tocopherols, were reduced in response to FD-infection. Thereafter, and consistently with the biochemical data, qPCR analysis highlighted a severe FD-mediated repression in key genes involved in isoprenoid biosynthetic pathways. A more diverse set of changes, on the contrary, was observed in the case of ABA metabolism. Principal component analysis (PCA) of all identified metabolites clearly separated healthy from FD-infected vines, therefore confirming that the infection strongly alters the biosynthesis of grapevine isoprenoids; additionally, forty-four genes and metabolites were identified as the components mostly explaining the variance between healthy and infected samples. Finally, transcriptmetabolite network correlation analyses were exploited to display the main hubs of the infection process, which highlighted a strong role of VvCHLG, VvVTE and VvZEP genes and the chlorophylls intermediates aminolevulunic acid and porphobilinogen in response to FD infection. Overall, results indicated that the FD infection impairs the synthesis of isoprenoids, through the repression of key genes involved in the biosynthesis of chlorophylls, carotenoids, quinones and tocopherols.The work was supported by National Funds by FCT - Portuguese Foundation for Science and Technology, under the strategic program UIDB/04050/2020. The work was also supported by FCT and European Funds (FEDER/POCI/ COMPETE2020) through the research projects BerryPlastid (PTDC/BIAFBT/28165/2017 and POCI-01-0145-FEDER - 028165), MitiVineDrought (PTDC/BIA-FBT/30341/2017 and POCI-01- 0145-FEDER-030341) and GrapeInfectomics (PTDC/ASPHOR/28485/2017). AT was supported by a postdoctoral researcher contract/position within the project “BerryPlastid”. HN was supported by an FCT postdoctoral grant (SFRH/BPD/115518/2016). The work also benefited from the networking activities within the European Cooperation in Science and Technology Action (EUROCAROTEN CA15136).info:eu-repo/semantics/publishedVersionFrontiers MediaUniversidade do MinhoTeixeira, AntónioMartins, VivianaFrusciante, SarahCruz, TelmoNoronha, HenriqueDiretto, GianfrancoGerós, H.2020-06-172020-06-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/65697engTeixeira, António; Martins, Viviana; Frusciante, Sarah; Cruz, Telmo; Noronha, Henrique; Diretto, Gianfranco; Gerós, Hernâni, Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways. Frontiers in Plant Science, 11(896), 20201664-462X10.3389/fpls.2020.00896http://www.frontiersin.org/Plant_Physiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T07:40:35Zoai:repositorium.sdum.uminho.pt:1822/65697Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T07:40:35Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
title |
Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
spellingShingle |
Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways Teixeira, António Vitis vinifera flavescence doree biotic stress leaf yellowing isoprenoids biosynthesis plant pathogens Science & Technology |
title_short |
Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
title_full |
Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
title_fullStr |
Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
title_full_unstemmed |
Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
title_sort |
Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways |
author |
Teixeira, António |
author_facet |
Teixeira, António Martins, Viviana Frusciante, Sarah Cruz, Telmo Noronha, Henrique Diretto, Gianfranco Gerós, H. |
author_role |
author |
author2 |
Martins, Viviana Frusciante, Sarah Cruz, Telmo Noronha, Henrique Diretto, Gianfranco Gerós, H. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Teixeira, António Martins, Viviana Frusciante, Sarah Cruz, Telmo Noronha, Henrique Diretto, Gianfranco Gerós, H. |
dc.subject.por.fl_str_mv |
Vitis vinifera flavescence doree biotic stress leaf yellowing isoprenoids biosynthesis plant pathogens Science & Technology |
topic |
Vitis vinifera flavescence doree biotic stress leaf yellowing isoprenoids biosynthesis plant pathogens Science & Technology |
description |
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2020.00896/full#supplementary-material |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-17 2020-06-17T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/65697 |
url |
http://hdl.handle.net/1822/65697 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Teixeira, António; Martins, Viviana; Frusciante, Sarah; Cruz, Telmo; Noronha, Henrique; Diretto, Gianfranco; Gerós, Hernâni, Flavescence dorée-derived leaf yellowing in grapevine (Vitis vinifera l.) is associated to a general repression of isoprenoid biosynthetic pathways. Frontiers in Plant Science, 11(896), 2020 1664-462X 10.3389/fpls.2020.00896 http://www.frontiersin.org/Plant_Physiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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mluisa.alvim@gmail.com |
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