Lab-on-valve automated and miniaturized assessment of nanoparticle concentration based on light-scattering
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/22719 |
Resumo: | Nanoparticles (NPs) concentration directly impacts the dose delivered to target tissues by nanocarriers. The evaluation of this parameter is required during NPs developmental and quality control stages, for setting dose−response correlations and for evaluating the reproduci bility of the manufacturing process. Still, faster and simpler procedures, dismissing skilled operators and post-analysis conversions are needed to quantify NPs for research and quality control operations, and to support result validation. Herein, a miniaturized automated ensemble method to measure NPs concentration was established under the lab-on-valve (LOV) mesofluidic platform. Automatic NPs sampling and delivery to the LOV detection unit were set by flow programming. NPs concentration measurements were based on the decrease in the light transmitted to the detector due to the light scattered by NPs when passing through the optical path. Each analysis was accomplished in 2 min, rendering a determination throughput of 30 h−1 (6 samples h−1 for n = 5) and only requiring 30 μL (≈0.03 g) of NPs suspension. Measurements were performed on polymeric NPs, as these represent one of the major classes of NPs under development for drug delivery aims. Determinations for polystyrene NPs (of 100, 200, and 500 nm) and for NPs made of PEGylated poly-D,L-lactide-co glycolide (PEG−PLGA, a biocompatible FDA-approved polymer) were accomplished within 108 −1012 particles mL−1 range, depending on the NPs size and composition. NPs size and concentration were maintained during analysis, as verified for NPs eluted from the LOV by particle tracking analysis (PTA). Moreover, concentration measurements for PEG−PLGA NPs loaded with an anti-inflammatory drug, methotrexate (MTX), after their incubation in simulated gastric and intestinal fluids were successfully achieved (recovery values of 102−115%, as confirmed by PTA), showing the suitability of the proposed method to support the development of polymeric NPs targeting intestinal delivery. |
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Lab-on-valve automated and miniaturized assessment of nanoparticle concentration based on light-scatteringAromatic compoundsFluidsHydrocarbonsLightNanoparticlesNanoparticles (NPs) concentration directly impacts the dose delivered to target tissues by nanocarriers. The evaluation of this parameter is required during NPs developmental and quality control stages, for setting dose−response correlations and for evaluating the reproduci bility of the manufacturing process. Still, faster and simpler procedures, dismissing skilled operators and post-analysis conversions are needed to quantify NPs for research and quality control operations, and to support result validation. Herein, a miniaturized automated ensemble method to measure NPs concentration was established under the lab-on-valve (LOV) mesofluidic platform. Automatic NPs sampling and delivery to the LOV detection unit were set by flow programming. NPs concentration measurements were based on the decrease in the light transmitted to the detector due to the light scattered by NPs when passing through the optical path. Each analysis was accomplished in 2 min, rendering a determination throughput of 30 h−1 (6 samples h−1 for n = 5) and only requiring 30 μL (≈0.03 g) of NPs suspension. Measurements were performed on polymeric NPs, as these represent one of the major classes of NPs under development for drug delivery aims. Determinations for polystyrene NPs (of 100, 200, and 500 nm) and for NPs made of PEGylated poly-D,L-lactide-co glycolide (PEG−PLGA, a biocompatible FDA-approved polymer) were accomplished within 108 −1012 particles mL−1 range, depending on the NPs size and composition. NPs size and concentration were maintained during analysis, as verified for NPs eluted from the LOV by particle tracking analysis (PTA). Moreover, concentration measurements for PEG−PLGA NPs loaded with an anti-inflammatory drug, methotrexate (MTX), after their incubation in simulated gastric and intestinal fluids were successfully achieved (recovery values of 102−115%, as confirmed by PTA), showing the suitability of the proposed method to support the development of polymeric NPs targeting intestinal delivery.ACS PublicationsRepositório Científico do Instituto Politécnico do PortoMarques, Sara S.Ramos, Inês I.Silva, CarlaBarreiros, LuisaDomingues, Maria R.Segundo, Marcela A.2023-04-12T15:20:06Z2023-02-212023-02-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/22719engMarques, S. S., Ramos, I. I., Silva, C., Barreiros, L., Domingues, M. R., & Segundo, M. A. (2023). Lab-on-Valve Automated and Miniaturized Assessment of Nanoparticle Concentration Based on Light-Scattering. Analytical Chemistry, 95(10), 4619–4626. https://doi.org/10.1021/acs.analchem.2c046310003-270010.1021/acs.analchem.2c046311520-6882metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-04-19T01:47:02Zoai:recipp.ipp.pt:10400.22/22719Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:49:41.863257Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Lab-on-valve automated and miniaturized assessment of nanoparticle concentration based on light-scattering |
title |
Lab-on-valve automated and miniaturized assessment of nanoparticle concentration based on light-scattering |
spellingShingle |
Lab-on-valve automated and miniaturized assessment of nanoparticle concentration based on light-scattering Marques, Sara S. Aromatic compounds Fluids Hydrocarbons Light Nanoparticles |
title_short |
Lab-on-valve automated and miniaturized assessment of nanoparticle concentration based on light-scattering |
title_full |
Lab-on-valve automated and miniaturized assessment of nanoparticle concentration based on light-scattering |
title_fullStr |
Lab-on-valve automated and miniaturized assessment of nanoparticle concentration based on light-scattering |
title_full_unstemmed |
Lab-on-valve automated and miniaturized assessment of nanoparticle concentration based on light-scattering |
title_sort |
Lab-on-valve automated and miniaturized assessment of nanoparticle concentration based on light-scattering |
author |
Marques, Sara S. |
author_facet |
Marques, Sara S. Ramos, Inês I. Silva, Carla Barreiros, Luisa Domingues, Maria R. Segundo, Marcela A. |
author_role |
author |
author2 |
Ramos, Inês I. Silva, Carla Barreiros, Luisa Domingues, Maria R. Segundo, Marcela A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Marques, Sara S. Ramos, Inês I. Silva, Carla Barreiros, Luisa Domingues, Maria R. Segundo, Marcela A. |
dc.subject.por.fl_str_mv |
Aromatic compounds Fluids Hydrocarbons Light Nanoparticles |
topic |
Aromatic compounds Fluids Hydrocarbons Light Nanoparticles |
description |
Nanoparticles (NPs) concentration directly impacts the dose delivered to target tissues by nanocarriers. The evaluation of this parameter is required during NPs developmental and quality control stages, for setting dose−response correlations and for evaluating the reproduci bility of the manufacturing process. Still, faster and simpler procedures, dismissing skilled operators and post-analysis conversions are needed to quantify NPs for research and quality control operations, and to support result validation. Herein, a miniaturized automated ensemble method to measure NPs concentration was established under the lab-on-valve (LOV) mesofluidic platform. Automatic NPs sampling and delivery to the LOV detection unit were set by flow programming. NPs concentration measurements were based on the decrease in the light transmitted to the detector due to the light scattered by NPs when passing through the optical path. Each analysis was accomplished in 2 min, rendering a determination throughput of 30 h−1 (6 samples h−1 for n = 5) and only requiring 30 μL (≈0.03 g) of NPs suspension. Measurements were performed on polymeric NPs, as these represent one of the major classes of NPs under development for drug delivery aims. Determinations for polystyrene NPs (of 100, 200, and 500 nm) and for NPs made of PEGylated poly-D,L-lactide-co glycolide (PEG−PLGA, a biocompatible FDA-approved polymer) were accomplished within 108 −1012 particles mL−1 range, depending on the NPs size and composition. NPs size and concentration were maintained during analysis, as verified for NPs eluted from the LOV by particle tracking analysis (PTA). Moreover, concentration measurements for PEG−PLGA NPs loaded with an anti-inflammatory drug, methotrexate (MTX), after their incubation in simulated gastric and intestinal fluids were successfully achieved (recovery values of 102−115%, as confirmed by PTA), showing the suitability of the proposed method to support the development of polymeric NPs targeting intestinal delivery. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-12T15:20:06Z 2023-02-21 2023-02-21T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/22719 |
url |
http://hdl.handle.net/10400.22/22719 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Marques, S. S., Ramos, I. I., Silva, C., Barreiros, L., Domingues, M. R., & Segundo, M. A. (2023). Lab-on-Valve Automated and Miniaturized Assessment of Nanoparticle Concentration Based on Light-Scattering. Analytical Chemistry, 95(10), 4619–4626. https://doi.org/10.1021/acs.analchem.2c04631 0003-2700 10.1021/acs.analchem.2c04631 1520-6882 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
ACS Publications |
publisher.none.fl_str_mv |
ACS Publications |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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