DNA imaged on a HOPG electrode surface by AFM with controlled potential
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5106 https://doi.org/10.1016/j.bioelechem.2004.05.009 |
Resumo: | Single-molecule AFM imaging of single-stranded and double-stranded DNA molecules self-assembled from solution onto a HOPG electrode surface is reported. The interaction of DNA with the hydrophobic surface induced DNA aggregation, overlapping, intra- and intermolecular interactions. Controlling the electrode potential and using the phase images as a control method, to confirm the correct topographical characterization, offers the possibility to enlarge the capability of AFM imaging of DNA immobilized onto conducting substrates, such as HOPG. The application of a potential of +300 mV (versus AgQRE) to the HOPG enhanced the robustness and stability of the adsorbed DNA molecules, increasing the electrostatic interaction between the positively charged electrode surface and the negatively charged DNA sugar-phosphate backbone. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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DNA imaged on a HOPG electrode surface by AFM with controlled potentialAdsorptionAtomic force microscopeSingle-molecule AFM imaging of single-stranded and double-stranded DNA molecules self-assembled from solution onto a HOPG electrode surface is reported. The interaction of DNA with the hydrophobic surface induced DNA aggregation, overlapping, intra- and intermolecular interactions. Controlling the electrode potential and using the phase images as a control method, to confirm the correct topographical characterization, offers the possibility to enlarge the capability of AFM imaging of DNA immobilized onto conducting substrates, such as HOPG. The application of a potential of +300 mV (versus AgQRE) to the HOPG enhanced the robustness and stability of the adsorbed DNA molecules, increasing the electrostatic interaction between the positively charged electrode surface and the negatively charged DNA sugar-phosphate backbone.http://www.sciencedirect.com/science/article/B6W72-4D5X9NS-1/1/d1528546598a5fb98abb8331a76055602005info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5106http://hdl.handle.net/10316/5106https://doi.org/10.1016/j.bioelechem.2004.05.009engBioelectrochemistry. 66:1-2 (2005) 117-124Brett, Ana Maria OliveiraPaquim, Ana-Maria Chiorceainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T17:00:32Zoai:estudogeral.uc.pt:10316/5106Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:38.520159Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
DNA imaged on a HOPG electrode surface by AFM with controlled potential |
title |
DNA imaged on a HOPG electrode surface by AFM with controlled potential |
spellingShingle |
DNA imaged on a HOPG electrode surface by AFM with controlled potential Brett, Ana Maria Oliveira Adsorption Atomic force microscope |
title_short |
DNA imaged on a HOPG electrode surface by AFM with controlled potential |
title_full |
DNA imaged on a HOPG electrode surface by AFM with controlled potential |
title_fullStr |
DNA imaged on a HOPG electrode surface by AFM with controlled potential |
title_full_unstemmed |
DNA imaged on a HOPG electrode surface by AFM with controlled potential |
title_sort |
DNA imaged on a HOPG electrode surface by AFM with controlled potential |
author |
Brett, Ana Maria Oliveira |
author_facet |
Brett, Ana Maria Oliveira Paquim, Ana-Maria Chiorcea |
author_role |
author |
author2 |
Paquim, Ana-Maria Chiorcea |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Brett, Ana Maria Oliveira Paquim, Ana-Maria Chiorcea |
dc.subject.por.fl_str_mv |
Adsorption Atomic force microscope |
topic |
Adsorption Atomic force microscope |
description |
Single-molecule AFM imaging of single-stranded and double-stranded DNA molecules self-assembled from solution onto a HOPG electrode surface is reported. The interaction of DNA with the hydrophobic surface induced DNA aggregation, overlapping, intra- and intermolecular interactions. Controlling the electrode potential and using the phase images as a control method, to confirm the correct topographical characterization, offers the possibility to enlarge the capability of AFM imaging of DNA immobilized onto conducting substrates, such as HOPG. The application of a potential of +300 mV (versus AgQRE) to the HOPG enhanced the robustness and stability of the adsorbed DNA molecules, increasing the electrostatic interaction between the positively charged electrode surface and the negatively charged DNA sugar-phosphate backbone. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5106 http://hdl.handle.net/10316/5106 https://doi.org/10.1016/j.bioelechem.2004.05.009 |
url |
http://hdl.handle.net/10316/5106 https://doi.org/10.1016/j.bioelechem.2004.05.009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioelectrochemistry. 66:1-2 (2005) 117-124 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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