Sensing of biorelevant molecules with metal-organic framework films
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/148365 |
Resumo: | Uric acid plays a major role in medical applications since its abnormal concentration translates into a diagnostic tool for clinical disorders such as gout, hyperuricemia or the Lesch–Nyhan syndrome. Therefore, the development of purine sensors became significant and relies on the fabrication of thin films and/or membranes based on nanomaterials. Metal-organic frame-works are constituted by organic linkers and metal ions. These have shown some promising results as sensors that can be attributed to their crystallinity, high and permanent porosity, and the presence of several active sites. This work will focus on Fe-MOF-74, with a crystal structure consisting of hexagonal channels and highly reactive metal sites. Metal-organic frameworks are conventionally prepared by solvothermal/hydrothermal methods, obtained as microcrystalline powders. These indirect techniques become limited by the complexity in casting the material to the desired shape. Direct techniques have been used to overcome these limitations, namely, the electrochemical method. This work reports the cathodic deposition of Fe-MOF-74 for the electrochemical detection of uric acid. Herein, pro-ligands were synthesised and characterised by nuclear magnetic resonance, Fourier transform infrared spectroscopy and elemental analysis. The electro-synthesised films were characterised by Fourier transform infrared spectroscopy, elemental analysis, atomic force microscopy, X-ray diffraction, Brunauer, Emmett and Teller surface area from N2 adsorption, scanning electron microscopy, particle-induced X-ray emission and Rutherford backscatter spectrometry. Finally, these films were applied to the electrochemical detection of uric acid and evaluated based on their limit of detection, sensitivity, and linear range. |
id |
RCAP_8148eff09e1f7c6d1c3ed7136dd19c40 |
---|---|
oai_identifier_str |
oai:run.unl.pt:10362/148365 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Sensing of biorelevant molecules with metal-organic framework filmsCyclic voltammetrycathodic depositionfilmsuric acidDomínio/Área Científica::Engenharia e Tecnologia::Engenharia QuímicaUric acid plays a major role in medical applications since its abnormal concentration translates into a diagnostic tool for clinical disorders such as gout, hyperuricemia or the Lesch–Nyhan syndrome. Therefore, the development of purine sensors became significant and relies on the fabrication of thin films and/or membranes based on nanomaterials. Metal-organic frame-works are constituted by organic linkers and metal ions. These have shown some promising results as sensors that can be attributed to their crystallinity, high and permanent porosity, and the presence of several active sites. This work will focus on Fe-MOF-74, with a crystal structure consisting of hexagonal channels and highly reactive metal sites. Metal-organic frameworks are conventionally prepared by solvothermal/hydrothermal methods, obtained as microcrystalline powders. These indirect techniques become limited by the complexity in casting the material to the desired shape. Direct techniques have been used to overcome these limitations, namely, the electrochemical method. This work reports the cathodic deposition of Fe-MOF-74 for the electrochemical detection of uric acid. Herein, pro-ligands were synthesised and characterised by nuclear magnetic resonance, Fourier transform infrared spectroscopy and elemental analysis. The electro-synthesised films were characterised by Fourier transform infrared spectroscopy, elemental analysis, atomic force microscopy, X-ray diffraction, Brunauer, Emmett and Teller surface area from N2 adsorption, scanning electron microscopy, particle-induced X-ray emission and Rutherford backscatter spectrometry. Finally, these films were applied to the electrochemical detection of uric acid and evaluated based on their limit of detection, sensitivity, and linear range.O ácido úrico desempenha um papel importante em aplicações medicinais, dado que a sua concentração anormal se traduz num diagnóstico de alterações clínicas, como a gota, hiperuricemia, ou a síndrome the Lesch-Nyhan. Posto isto, o desenvolvimento de sensores de purinas tornou-se significante, estando dependente do fabrico de filmes finos e/ou membranas baseados/as em nanomateriais. As redes metal-orgânicas são constituídas por ligandos orgânicos e iões metálicos. Estas têm vindo a apresentar resultados promissores enquanto sensores, atribuídos à sua elevada cristalinidade e porosidade, assim como à presença de vários sítios ativos. Este trabalho será focado na Fe-MOF-74, que apresenta uma estrutura cristalina constituída por canais hexagonais e centros metálicos altamente reativos. As redes metal-orgânicas são convencionalmente preparadas através de métodos solvotérmicos/hidrotérmicos, obtendo-as na forma de pó microcristalino. Estas técnicas indiretas tornam-se limitadas pela complexidade na moldagem dos materiais na forma desejada. As técnicas diretas têm vindo a colmatar estas limitações, nomeadamente, o método eletroquímico. Este trabalho reporta a deposição catódica da Fe-MOF-74 e derivados para a deteção eletroquímica de ácido úrico. Para tal, pró-ligandos foram sintetizados e caracterizados através de ressonância magnética nuclear, espetroscopia de infravermelho com transformada de Fourier e análise elementar. Os filmes eletrosintetisados foram caracterizados através de espetroscopia de infravermelho com transformada de Fourier, análise elementar, espetroscopia de força atómica, difração de raios-X, área superficial de Brunauer, Emmett and Teller obtida através da adsorção de N2, microscopia eletrónica de varrimento, emissão de raios-X induzida por partículas e espectrometria de retroespalhamento de Rutherford. Por fim, estes filmes foram aplicados na deteção eletroquímica de ácido úrico avaliados com base no limite de deteção , sensibilidade e gama linear.Martinho, PauloGomes, ClaraRUNReis, Ana Rita2023-01-30T10:19:54Z2022-112022-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/148365enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:29:45Zoai:run.unl.pt:10362/148365Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:53:19.798009Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Sensing of biorelevant molecules with metal-organic framework films |
title |
Sensing of biorelevant molecules with metal-organic framework films |
spellingShingle |
Sensing of biorelevant molecules with metal-organic framework films Reis, Ana Rita Cyclic voltammetry cathodic deposition films uric acid Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química |
title_short |
Sensing of biorelevant molecules with metal-organic framework films |
title_full |
Sensing of biorelevant molecules with metal-organic framework films |
title_fullStr |
Sensing of biorelevant molecules with metal-organic framework films |
title_full_unstemmed |
Sensing of biorelevant molecules with metal-organic framework films |
title_sort |
Sensing of biorelevant molecules with metal-organic framework films |
author |
Reis, Ana Rita |
author_facet |
Reis, Ana Rita |
author_role |
author |
dc.contributor.none.fl_str_mv |
Martinho, Paulo Gomes, Clara RUN |
dc.contributor.author.fl_str_mv |
Reis, Ana Rita |
dc.subject.por.fl_str_mv |
Cyclic voltammetry cathodic deposition films uric acid Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química |
topic |
Cyclic voltammetry cathodic deposition films uric acid Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química |
description |
Uric acid plays a major role in medical applications since its abnormal concentration translates into a diagnostic tool for clinical disorders such as gout, hyperuricemia or the Lesch–Nyhan syndrome. Therefore, the development of purine sensors became significant and relies on the fabrication of thin films and/or membranes based on nanomaterials. Metal-organic frame-works are constituted by organic linkers and metal ions. These have shown some promising results as sensors that can be attributed to their crystallinity, high and permanent porosity, and the presence of several active sites. This work will focus on Fe-MOF-74, with a crystal structure consisting of hexagonal channels and highly reactive metal sites. Metal-organic frameworks are conventionally prepared by solvothermal/hydrothermal methods, obtained as microcrystalline powders. These indirect techniques become limited by the complexity in casting the material to the desired shape. Direct techniques have been used to overcome these limitations, namely, the electrochemical method. This work reports the cathodic deposition of Fe-MOF-74 for the electrochemical detection of uric acid. Herein, pro-ligands were synthesised and characterised by nuclear magnetic resonance, Fourier transform infrared spectroscopy and elemental analysis. The electro-synthesised films were characterised by Fourier transform infrared spectroscopy, elemental analysis, atomic force microscopy, X-ray diffraction, Brunauer, Emmett and Teller surface area from N2 adsorption, scanning electron microscopy, particle-induced X-ray emission and Rutherford backscatter spectrometry. Finally, these films were applied to the electrochemical detection of uric acid and evaluated based on their limit of detection, sensitivity, and linear range. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11 2022-11-01T00:00:00Z 2023-01-30T10:19:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/148365 |
url |
http://hdl.handle.net/10362/148365 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799138123861458944 |