Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) applied to diatom identification: influence of culturing age
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31797 |
Resumo: | Diatom identification is based on classical morphological methods, focused on both the frustule shape and ornamentation analysis and possible chain-forming. Being many morphological aspects common to several species, diatom identification and characterisation is both time-consuming and high expertise demanding. Furthermore, the use of advanced microscopy, such as transmission electron microscopy (TEM) or Scanning Electron Microscopy (SEM) is frequently necessary to differentiate these organisms. In this work, the matrix-assisted laser desorption /ionisation time-of-flight mass spectrometry (MALDI-TOF MS) was used to differentiate diatom taxa in a preliminary study of the possibility of using this technique to obtain reliable fingerprinting of diatoms in the range 2 to 20 KDa. Seminavis robusta, Coscinodiscus sp., Thalassiosira sp. and Cyclotella meneghiniana were used. The influence of culture age was studied concerning reproducibility. It was shown that MALDI-TOF MS spectra varied with culturing time and the need of taking this in considerations when setting up experimental standard conditions in the identification of diatoms is clearly established. One of the most important observations was that each diatom strain presented a specific age from where the mass spectrum became reproducible. For all Seminavis robusta strains and for Cyclotella meneghiniana this age was 9 days whereas for Thalassiosira sp. and Coscinodiscus sp. this limit was 13 days. Even with the limitations related with culture aging versus spectra reproducibility, MALDI-TOF MS proved to be a useful complementary tool to identify and characterise diatoms at the level of genus. However, it seems that it was not possible to distinguish different mating types inside the same species, as evidenced by the results obtained with Seminavis robusta isolates. |
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Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) applied to diatom identification: influence of culturing ageDiatomsMALDI-TOF MSCulture ageScience & TechnologyDiatom identification is based on classical morphological methods, focused on both the frustule shape and ornamentation analysis and possible chain-forming. Being many morphological aspects common to several species, diatom identification and characterisation is both time-consuming and high expertise demanding. Furthermore, the use of advanced microscopy, such as transmission electron microscopy (TEM) or Scanning Electron Microscopy (SEM) is frequently necessary to differentiate these organisms. In this work, the matrix-assisted laser desorption /ionisation time-of-flight mass spectrometry (MALDI-TOF MS) was used to differentiate diatom taxa in a preliminary study of the possibility of using this technique to obtain reliable fingerprinting of diatoms in the range 2 to 20 KDa. Seminavis robusta, Coscinodiscus sp., Thalassiosira sp. and Cyclotella meneghiniana were used. The influence of culture age was studied concerning reproducibility. It was shown that MALDI-TOF MS spectra varied with culturing time and the need of taking this in considerations when setting up experimental standard conditions in the identification of diatoms is clearly established. One of the most important observations was that each diatom strain presented a specific age from where the mass spectrum became reproducible. For all Seminavis robusta strains and for Cyclotella meneghiniana this age was 9 days whereas for Thalassiosira sp. and Coscinodiscus sp. this limit was 13 days. Even with the limitations related with culture aging versus spectra reproducibility, MALDI-TOF MS proved to be a useful complementary tool to identify and characterise diatoms at the level of genus. However, it seems that it was not possible to distinguish different mating types inside the same species, as evidenced by the results obtained with Seminavis robusta isolates.The authors sincerely acknowledge W. Vyverman and P. Vanormelingen for kindly providing the diatom cultures. The authors thank the FCT Strategic Project PEst-OE/EQB/LA0023/2013, the FCT Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462) and the Project 'BioENV - Biotechnology and Bioengineering for a sustainable word', REF. NORTE-07-0124-FEDER-000048, co-funded by the Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER.Inter-ResearchUniversidade do MinhoNicolau, AnaSequeira, Lídia SofiaSantos, C.Mota, M.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31797eng1864-77901864-778210.3354/ab00548info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:35:59Zoai:repositorium.sdum.uminho.pt:1822/31797Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:31:56.895760Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) applied to diatom identification: influence of culturing age |
title |
Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) applied to diatom identification: influence of culturing age |
spellingShingle |
Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) applied to diatom identification: influence of culturing age Nicolau, Ana Diatoms MALDI-TOF MS Culture age Science & Technology |
title_short |
Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) applied to diatom identification: influence of culturing age |
title_full |
Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) applied to diatom identification: influence of culturing age |
title_fullStr |
Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) applied to diatom identification: influence of culturing age |
title_full_unstemmed |
Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) applied to diatom identification: influence of culturing age |
title_sort |
Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) applied to diatom identification: influence of culturing age |
author |
Nicolau, Ana |
author_facet |
Nicolau, Ana Sequeira, Lídia Sofia Santos, C. Mota, M. |
author_role |
author |
author2 |
Sequeira, Lídia Sofia Santos, C. Mota, M. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Nicolau, Ana Sequeira, Lídia Sofia Santos, C. Mota, M. |
dc.subject.por.fl_str_mv |
Diatoms MALDI-TOF MS Culture age Science & Technology |
topic |
Diatoms MALDI-TOF MS Culture age Science & Technology |
description |
Diatom identification is based on classical morphological methods, focused on both the frustule shape and ornamentation analysis and possible chain-forming. Being many morphological aspects common to several species, diatom identification and characterisation is both time-consuming and high expertise demanding. Furthermore, the use of advanced microscopy, such as transmission electron microscopy (TEM) or Scanning Electron Microscopy (SEM) is frequently necessary to differentiate these organisms. In this work, the matrix-assisted laser desorption /ionisation time-of-flight mass spectrometry (MALDI-TOF MS) was used to differentiate diatom taxa in a preliminary study of the possibility of using this technique to obtain reliable fingerprinting of diatoms in the range 2 to 20 KDa. Seminavis robusta, Coscinodiscus sp., Thalassiosira sp. and Cyclotella meneghiniana were used. The influence of culture age was studied concerning reproducibility. It was shown that MALDI-TOF MS spectra varied with culturing time and the need of taking this in considerations when setting up experimental standard conditions in the identification of diatoms is clearly established. One of the most important observations was that each diatom strain presented a specific age from where the mass spectrum became reproducible. For all Seminavis robusta strains and for Cyclotella meneghiniana this age was 9 days whereas for Thalassiosira sp. and Coscinodiscus sp. this limit was 13 days. Even with the limitations related with culture aging versus spectra reproducibility, MALDI-TOF MS proved to be a useful complementary tool to identify and characterise diatoms at the level of genus. However, it seems that it was not possible to distinguish different mating types inside the same species, as evidenced by the results obtained with Seminavis robusta isolates. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31797 |
url |
http://hdl.handle.net/1822/31797 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1864-7790 1864-7782 10.3354/ab00548 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Inter-Research |
publisher.none.fl_str_mv |
Inter-Research |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132830095114240 |