Adaptation to β-myrcene catabolism in Pseudomonas sp. M1: An expression proteomics analysis
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/87001 |
Resumo: | β-Myrcene, a monoterpene widely used as a fragrance and flavoring additive, also possesses analgesic, anti-mutagenic, and tyrosinase inhibitory properties. In order to get insights into the molecular mechanisms underlying the ability of Pseudomonas sp. M1 to catabolize β-myrcene, an expression proteomics approach was used in this study. Results indicate that the catabolic enzyme machinery for β-myrcene utilization (MyrB, MyrC, and MyrD and other uncharacterized proteins) is strongly induced when β-myrcene is present in the growth medium. Since an M1 mutant, lacking a functional 2-methylisocitrate dehydratase, is not able to grow in mineral medium with β-myrcene or propionic acid as the sole C-source, and also based on the expression proteomic analysis carried out in this study, it is suggested that the β-myrcene catabolic intermediate propionyl-CoA is channeled into the central metabolism via the 2-methylcitrate cycle. Results also suggest that the major alteration occurring in the central carbon metabolism of cells growing in β-myrcene-containing media is related with the redistribution of the metabolic fluxes leading to increased oxaloacetate production. Other upregulated proteins are believed to prevent protein misfolding and aggregation or to play important structural roles, contributing to the adaptive alteration of cell wall and membrane organization and integrity, which are essential features to allow the bacterium to cope with the highly lipophilic β-myrcene as C-source. |
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Adaptation to β-myrcene catabolism in Pseudomonas sp. M1: An expression proteomics analysisß-myrceneß-myrcene catabolismEssential oilsExpression proteomicsMicrobiologyPseudomonasβ-myrceneβ-myrcene catabolismCiências Naturais::Ciências Biológicasβ-Myrcene, a monoterpene widely used as a fragrance and flavoring additive, also possesses analgesic, anti-mutagenic, and tyrosinase inhibitory properties. In order to get insights into the molecular mechanisms underlying the ability of Pseudomonas sp. M1 to catabolize β-myrcene, an expression proteomics approach was used in this study. Results indicate that the catabolic enzyme machinery for β-myrcene utilization (MyrB, MyrC, and MyrD and other uncharacterized proteins) is strongly induced when β-myrcene is present in the growth medium. Since an M1 mutant, lacking a functional 2-methylisocitrate dehydratase, is not able to grow in mineral medium with β-myrcene or propionic acid as the sole C-source, and also based on the expression proteomic analysis carried out in this study, it is suggested that the β-myrcene catabolic intermediate propionyl-CoA is channeled into the central metabolism via the 2-methylcitrate cycle. Results also suggest that the major alteration occurring in the central carbon metabolism of cells growing in β-myrcene-containing media is related with the redistribution of the metabolic fluxes leading to increased oxaloacetate production. Other upregulated proteins are believed to prevent protein misfolding and aggregation or to play important structural roles, contributing to the adaptive alteration of cell wall and membrane organization and integrity, which are essential features to allow the bacterium to cope with the highly lipophilic β-myrcene as C-source.- (undefined)WileyUniversidade do MinhoSantos, P. M.Sá-Correia, Isabel2009-112009-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/87001engSantos, P. M., & Sá‐Correia, I. (2009, November). Adaptation to β‐myrcene catabolism in Pseudomonas sp. M1: An expression proteomics analysis. Proteomics. Wiley. http://doi.org/10.1002/pmic.2009003251615-98531615-986110.1002/pmic.2009003251979867219798672https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/pmic.200900325info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-21T01:27:52Zoai:repositorium.sdum.uminho.pt:1822/87001Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:39:07.375211Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Adaptation to β-myrcene catabolism in Pseudomonas sp. M1: An expression proteomics analysis |
title |
Adaptation to β-myrcene catabolism in Pseudomonas sp. M1: An expression proteomics analysis |
spellingShingle |
Adaptation to β-myrcene catabolism in Pseudomonas sp. M1: An expression proteomics analysis Santos, P. M. ß-myrcene ß-myrcene catabolism Essential oils Expression proteomics Microbiology Pseudomonas β-myrcene β-myrcene catabolism Ciências Naturais::Ciências Biológicas |
title_short |
Adaptation to β-myrcene catabolism in Pseudomonas sp. M1: An expression proteomics analysis |
title_full |
Adaptation to β-myrcene catabolism in Pseudomonas sp. M1: An expression proteomics analysis |
title_fullStr |
Adaptation to β-myrcene catabolism in Pseudomonas sp. M1: An expression proteomics analysis |
title_full_unstemmed |
Adaptation to β-myrcene catabolism in Pseudomonas sp. M1: An expression proteomics analysis |
title_sort |
Adaptation to β-myrcene catabolism in Pseudomonas sp. M1: An expression proteomics analysis |
author |
Santos, P. M. |
author_facet |
Santos, P. M. Sá-Correia, Isabel |
author_role |
author |
author2 |
Sá-Correia, Isabel |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Santos, P. M. Sá-Correia, Isabel |
dc.subject.por.fl_str_mv |
ß-myrcene ß-myrcene catabolism Essential oils Expression proteomics Microbiology Pseudomonas β-myrcene β-myrcene catabolism Ciências Naturais::Ciências Biológicas |
topic |
ß-myrcene ß-myrcene catabolism Essential oils Expression proteomics Microbiology Pseudomonas β-myrcene β-myrcene catabolism Ciências Naturais::Ciências Biológicas |
description |
β-Myrcene, a monoterpene widely used as a fragrance and flavoring additive, also possesses analgesic, anti-mutagenic, and tyrosinase inhibitory properties. In order to get insights into the molecular mechanisms underlying the ability of Pseudomonas sp. M1 to catabolize β-myrcene, an expression proteomics approach was used in this study. Results indicate that the catabolic enzyme machinery for β-myrcene utilization (MyrB, MyrC, and MyrD and other uncharacterized proteins) is strongly induced when β-myrcene is present in the growth medium. Since an M1 mutant, lacking a functional 2-methylisocitrate dehydratase, is not able to grow in mineral medium with β-myrcene or propionic acid as the sole C-source, and also based on the expression proteomic analysis carried out in this study, it is suggested that the β-myrcene catabolic intermediate propionyl-CoA is channeled into the central metabolism via the 2-methylcitrate cycle. Results also suggest that the major alteration occurring in the central carbon metabolism of cells growing in β-myrcene-containing media is related with the redistribution of the metabolic fluxes leading to increased oxaloacetate production. Other upregulated proteins are believed to prevent protein misfolding and aggregation or to play important structural roles, contributing to the adaptive alteration of cell wall and membrane organization and integrity, which are essential features to allow the bacterium to cope with the highly lipophilic β-myrcene as C-source. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11 2009-11-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/87001 |
url |
https://hdl.handle.net/1822/87001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Santos, P. M., & Sá‐Correia, I. (2009, November). Adaptation to β‐myrcene catabolism in Pseudomonas sp. M1: An expression proteomics analysis. Proteomics. Wiley. http://doi.org/10.1002/pmic.200900325 1615-9853 1615-9861 10.1002/pmic.200900325 19798672 19798672 https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/pmic.200900325 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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