Biofilm formation of multidrug-resistant MRSA strains isolated from different types of human infections
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/73814 |
Resumo: | Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main pathogens causing chronic infections, mainly due to its capacity to form biofilms. However, the mechanisms underlying the biofilm formation of MRSA strains from different types of human infections are not fully understood. MRSA strains isolated from distinct human infections were characterized aiming to determine their biofilm-forming capacity, the biofilm resistance to conventional antibiotics and the prevalence of biofilm-related genes, including, icaA, icaB, icaC, icaD, fnbA, fnbB, clfA, clfB, cna, eno, ebpS, fib and bbp. Eighty-three clinical MRSA strains recovered from bacteremia episodes, osteomyelitis and diabetic foot ulcers were used. The biofilm-forming capacity was evaluated by the microtiter biofilm assay and the biofilm structure was analyzed via confocal scanning laser microscopy. The antimicrobial susceptibility of 24-h-old biofilms was assessed against three antibiotics and the biomass reduction was measured. The metabolic activity of biofilms was evaluated by the XTT assay. The presence of biofilm-related genes was investigated by whole-genome sequencing and by PCR. Despite different intensities, all strains showed the capacity to form biofilms. Most strains had also a large number of biofilm-related genes. However, strains isolated from osteomyelitis showed a lower capacity to form biofilms and also a lower prevalence of biofilm-associated genes. There was a significant reduction in the biofilm biomass of some strains tested against antibiotics. Our results provide important information on the biofilm-forming capacity of clinical MRSA strains, which may be essential to understand the influence of different types of infections on biofilm production and chronic infections. |
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Biofilm formation of multidrug-resistant MRSA strains isolated from different types of human infectionsMRSAHuman infectionsBiofilmsBiofilm-related genesScience & TechnologyMethicillin-resistant Staphylococcus aureus (MRSA) is one of the main pathogens causing chronic infections, mainly due to its capacity to form biofilms. However, the mechanisms underlying the biofilm formation of MRSA strains from different types of human infections are not fully understood. MRSA strains isolated from distinct human infections were characterized aiming to determine their biofilm-forming capacity, the biofilm resistance to conventional antibiotics and the prevalence of biofilm-related genes, including, icaA, icaB, icaC, icaD, fnbA, fnbB, clfA, clfB, cna, eno, ebpS, fib and bbp. Eighty-three clinical MRSA strains recovered from bacteremia episodes, osteomyelitis and diabetic foot ulcers were used. The biofilm-forming capacity was evaluated by the microtiter biofilm assay and the biofilm structure was analyzed via confocal scanning laser microscopy. The antimicrobial susceptibility of 24-h-old biofilms was assessed against three antibiotics and the biomass reduction was measured. The metabolic activity of biofilms was evaluated by the XTT assay. The presence of biofilm-related genes was investigated by whole-genome sequencing and by PCR. Despite different intensities, all strains showed the capacity to form biofilms. Most strains had also a large number of biofilm-related genes. However, strains isolated from osteomyelitis showed a lower capacity to form biofilms and also a lower prevalence of biofilm-associated genes. There was a significant reduction in the biofilm biomass of some strains tested against antibiotics. Our results provide important information on the biofilm-forming capacity of clinical MRSA strains, which may be essential to understand the influence of different types of infections on biofilm production and chronic infections.This work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative thera-nostic biomarkers, with reference NORTE-01-0145-FEDER-030101 and PTDC/SAU-INF/30101/2017, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). This work was supported by the Associate Laboratory for Green Chemistry—LAQV which is financed by national funds from FCT/MCTES (UIDB/50006/2020 and UIDP/50006/2020). Vanessa Silva is grateful to FCT (Fundação para a Ciência e a Tecnologia) for financial support through the PhD grant SFRH/BD/137947/2018.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoSilva, VanessaAlmeida, Luciana Mayara MendonçaGaio, Vânia SilvaCerca, NunoManageiro, VeraCaniça, ManuelaCapelo, José L.Igrejas, GilbertoPoeta, Patrícia2021-072021-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/73814engSilva, Vanessa; Almeida, Luciana; Gaio, Vania; Cerca, Nuno; Manageiro, Vera; Caniça, Manuela; Capelo, José L.; Igrejas, Gilberto; Poeta, Patrícia, Biofilm formation of multidrug-resistant MRSA strains isolated from different types of human infections. Pathogens, 10(8), 20212076-081710.3390/pathogens10080970http://www.mdpi.com/journal/pathogensinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:28:48Zoai:repositorium.sdum.uminho.pt:1822/73814Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:23:41.132121Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Biofilm formation of multidrug-resistant MRSA strains isolated from different types of human infections |
title |
Biofilm formation of multidrug-resistant MRSA strains isolated from different types of human infections |
spellingShingle |
Biofilm formation of multidrug-resistant MRSA strains isolated from different types of human infections Silva, Vanessa MRSA Human infections Biofilms Biofilm-related genes Science & Technology |
title_short |
Biofilm formation of multidrug-resistant MRSA strains isolated from different types of human infections |
title_full |
Biofilm formation of multidrug-resistant MRSA strains isolated from different types of human infections |
title_fullStr |
Biofilm formation of multidrug-resistant MRSA strains isolated from different types of human infections |
title_full_unstemmed |
Biofilm formation of multidrug-resistant MRSA strains isolated from different types of human infections |
title_sort |
Biofilm formation of multidrug-resistant MRSA strains isolated from different types of human infections |
author |
Silva, Vanessa |
author_facet |
Silva, Vanessa Almeida, Luciana Mayara Mendonça Gaio, Vânia Silva Cerca, Nuno Manageiro, Vera Caniça, Manuela Capelo, José L. Igrejas, Gilberto Poeta, Patrícia |
author_role |
author |
author2 |
Almeida, Luciana Mayara Mendonça Gaio, Vânia Silva Cerca, Nuno Manageiro, Vera Caniça, Manuela Capelo, José L. Igrejas, Gilberto Poeta, Patrícia |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva, Vanessa Almeida, Luciana Mayara Mendonça Gaio, Vânia Silva Cerca, Nuno Manageiro, Vera Caniça, Manuela Capelo, José L. Igrejas, Gilberto Poeta, Patrícia |
dc.subject.por.fl_str_mv |
MRSA Human infections Biofilms Biofilm-related genes Science & Technology |
topic |
MRSA Human infections Biofilms Biofilm-related genes Science & Technology |
description |
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main pathogens causing chronic infections, mainly due to its capacity to form biofilms. However, the mechanisms underlying the biofilm formation of MRSA strains from different types of human infections are not fully understood. MRSA strains isolated from distinct human infections were characterized aiming to determine their biofilm-forming capacity, the biofilm resistance to conventional antibiotics and the prevalence of biofilm-related genes, including, icaA, icaB, icaC, icaD, fnbA, fnbB, clfA, clfB, cna, eno, ebpS, fib and bbp. Eighty-three clinical MRSA strains recovered from bacteremia episodes, osteomyelitis and diabetic foot ulcers were used. The biofilm-forming capacity was evaluated by the microtiter biofilm assay and the biofilm structure was analyzed via confocal scanning laser microscopy. The antimicrobial susceptibility of 24-h-old biofilms was assessed against three antibiotics and the biomass reduction was measured. The metabolic activity of biofilms was evaluated by the XTT assay. The presence of biofilm-related genes was investigated by whole-genome sequencing and by PCR. Despite different intensities, all strains showed the capacity to form biofilms. Most strains had also a large number of biofilm-related genes. However, strains isolated from osteomyelitis showed a lower capacity to form biofilms and also a lower prevalence of biofilm-associated genes. There was a significant reduction in the biofilm biomass of some strains tested against antibiotics. Our results provide important information on the biofilm-forming capacity of clinical MRSA strains, which may be essential to understand the influence of different types of infections on biofilm production and chronic infections. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07 2021-07-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/73814 |
url |
http://hdl.handle.net/1822/73814 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva, Vanessa; Almeida, Luciana; Gaio, Vania; Cerca, Nuno; Manageiro, Vera; Caniça, Manuela; Capelo, José L.; Igrejas, Gilberto; Poeta, Patrícia, Biofilm formation of multidrug-resistant MRSA strains isolated from different types of human infections. Pathogens, 10(8), 2021 2076-0817 10.3390/pathogens10080970 http://www.mdpi.com/journal/pathogens |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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