Protein matrices for improved wound healing : elastase inhibition by a synthetic peptide model

Detalhes bibliográficos
Autor(a) principal: Vasconcelos, Andreia
Data de Publicação: 2010
Outros Autores: Pêgo, A. P., Lamghari, M., Henriques, L., Paulo, Artur Cavaco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/17323
Resumo: The unique properties of silk fibroin were combined with keratin to develop new wound-dressing materials. Silk fibroin/keratin (SF/K) films were prepared to reduce high levels of elastase found on chronic wounds. This improved biological function was achieved by the incorporation of a small peptide synthesized based on the reactive-site loop of the Bowman−Birk Inhibitor (BBI) protein. In vitro degradation and release were evaluated using porcine pancreatic elastase (PPE) solution as a model of wound exudate. It was found that biological degradation and release rate are highly dependent on film composition. Furthermore, the level of PPE activity can be tuned by changing the film composition, thus showing an innovative way of controlling the elastase−antielastase imbalance found on chronic wounds.
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spelling Protein matrices for improved wound healing : elastase inhibition by a synthetic peptide modelScience & TechnologyThe unique properties of silk fibroin were combined with keratin to develop new wound-dressing materials. Silk fibroin/keratin (SF/K) films were prepared to reduce high levels of elastase found on chronic wounds. This improved biological function was achieved by the incorporation of a small peptide synthesized based on the reactive-site loop of the Bowman−Birk Inhibitor (BBI) protein. In vitro degradation and release were evaluated using porcine pancreatic elastase (PPE) solution as a model of wound exudate. It was found that biological degradation and release rate are highly dependent on film composition. Furthermore, the level of PPE activity can be tuned by changing the film composition, thus showing an innovative way of controlling the elastase−antielastase imbalance found on chronic wounds.We would like to acknowledge FCT - Portuguese Foundation for Science and Technology for the scholarship concession; European project Lidwine, contract no. NMP2-CT-2006-026741, and Silvia Cappellozza from "Sezione Specializzata per la Bachicoltura" for the supply of silk cocoons.ACS PublicationsUniversidade do MinhoVasconcelos, AndreiaPêgo, A. P.Lamghari, M.Henriques, L.Paulo, Artur Cavaco20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/17323eng1525-779710.1021/bm100537b20690591info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:28:03Zoai:repositorium.sdum.uminho.pt:1822/17323Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:22:46.571533Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Protein matrices for improved wound healing : elastase inhibition by a synthetic peptide model
title Protein matrices for improved wound healing : elastase inhibition by a synthetic peptide model
spellingShingle Protein matrices for improved wound healing : elastase inhibition by a synthetic peptide model
Vasconcelos, Andreia
Science & Technology
title_short Protein matrices for improved wound healing : elastase inhibition by a synthetic peptide model
title_full Protein matrices for improved wound healing : elastase inhibition by a synthetic peptide model
title_fullStr Protein matrices for improved wound healing : elastase inhibition by a synthetic peptide model
title_full_unstemmed Protein matrices for improved wound healing : elastase inhibition by a synthetic peptide model
title_sort Protein matrices for improved wound healing : elastase inhibition by a synthetic peptide model
author Vasconcelos, Andreia
author_facet Vasconcelos, Andreia
Pêgo, A. P.
Lamghari, M.
Henriques, L.
Paulo, Artur Cavaco
author_role author
author2 Pêgo, A. P.
Lamghari, M.
Henriques, L.
Paulo, Artur Cavaco
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Vasconcelos, Andreia
Pêgo, A. P.
Lamghari, M.
Henriques, L.
Paulo, Artur Cavaco
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description The unique properties of silk fibroin were combined with keratin to develop new wound-dressing materials. Silk fibroin/keratin (SF/K) films were prepared to reduce high levels of elastase found on chronic wounds. This improved biological function was achieved by the incorporation of a small peptide synthesized based on the reactive-site loop of the Bowman−Birk Inhibitor (BBI) protein. In vitro degradation and release were evaluated using porcine pancreatic elastase (PPE) solution as a model of wound exudate. It was found that biological degradation and release rate are highly dependent on film composition. Furthermore, the level of PPE activity can be tuned by changing the film composition, thus showing an innovative way of controlling the elastase−antielastase imbalance found on chronic wounds.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/17323
url http://hdl.handle.net/1822/17323
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1525-7797
10.1021/bm100537b
20690591
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ACS Publications
publisher.none.fl_str_mv ACS Publications
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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