Rat olfactory mucosa mesenchymal stem/stromal cells (om-mscs): a characterization study
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/30279 |
Resumo: | Stem/stromal cell-based therapies are a branch of regenerative medicine and stand as an attractive option to promote the repair of damaged or dysfunctional tissues and organs. Olfactory mucosa mesenchymal stem/stromal cells have been regarded as a promising tool in regenerative therapies because of their several favorable properties such as multipotency, high proliferation rate, helpful location, and few associated ethical issues. These cells are easily accessible in the nasal cavity of most mammals, including the rat, can be easily applied in autologous treatments, and do not cope with most of the obstacles associated with the use of other stem cells. Despite this, its application in preclinical trials and in both human and animal patients is still limited because of the small number of studies performed so far and to the nonexistence of a standard and unambiguous protocol for collection, isolation, and therapeutic application. In the present work a validation of a protocol for isolation, culture, expansion, freezing, and thawing of olfactory mucosa mesenchymal stem/stromal cells was performed, applied to the rat model, as well as a biological characterization of these cells. To investigate the therapeutic potential of OM-MSCs and their eventual safe application in preclinical trials, the main characteristics of OMSC stemness were addressed. |
id |
RCAP_8237ad98b337f01ac6f1770daf21a9b3 |
---|---|
oai_identifier_str |
oai:repositorio.ucp.pt:10400.14/30279 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Rat olfactory mucosa mesenchymal stem/stromal cells (om-mscs): a characterization studyStem/stromal cell-based therapies are a branch of regenerative medicine and stand as an attractive option to promote the repair of damaged or dysfunctional tissues and organs. Olfactory mucosa mesenchymal stem/stromal cells have been regarded as a promising tool in regenerative therapies because of their several favorable properties such as multipotency, high proliferation rate, helpful location, and few associated ethical issues. These cells are easily accessible in the nasal cavity of most mammals, including the rat, can be easily applied in autologous treatments, and do not cope with most of the obstacles associated with the use of other stem cells. Despite this, its application in preclinical trials and in both human and animal patients is still limited because of the small number of studies performed so far and to the nonexistence of a standard and unambiguous protocol for collection, isolation, and therapeutic application. In the present work a validation of a protocol for isolation, culture, expansion, freezing, and thawing of olfactory mucosa mesenchymal stem/stromal cells was performed, applied to the rat model, as well as a biological characterization of these cells. To investigate the therapeutic potential of OM-MSCs and their eventual safe application in preclinical trials, the main characteristics of OMSC stemness were addressed.HindawiVeritati - Repositório Institucional da Universidade Católica PortuguesaAlvites, Rui D.Branquinho, Mariana V.Caseiro, Ana R.Amorim, IrinaPedrosa, Sílvia SantosRêma, AlexandraFaria, FátimaPorto, BeatrizOliveira, CláudiaTeixeira, PaulaMagalhães, RuiGeuna, StefanoVarejão, Artur S. P.Maurício, Ana C.2020-04-17T14:04:32Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/30279engAlvites, R. D., Branquinho, M. V., Caseiro, A. R., Amorim, I., Santos Pedrosa, S., Rêma, A., ..., Magalhães, R. (2020). Rat Olfactory Mucosa Mesenchymal Stem/Stromal Cells (OM-MSCs): A Characterization Study. International Journal of Cell Biology1687-887610.1155/2020/29382581687-888485079295088PMC721232432411249info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-05T01:36:46Zoai:repositorio.ucp.pt:10400.14/30279Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:24:24.145768Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Rat olfactory mucosa mesenchymal stem/stromal cells (om-mscs): a characterization study |
title |
Rat olfactory mucosa mesenchymal stem/stromal cells (om-mscs): a characterization study |
spellingShingle |
Rat olfactory mucosa mesenchymal stem/stromal cells (om-mscs): a characterization study Alvites, Rui D. |
title_short |
Rat olfactory mucosa mesenchymal stem/stromal cells (om-mscs): a characterization study |
title_full |
Rat olfactory mucosa mesenchymal stem/stromal cells (om-mscs): a characterization study |
title_fullStr |
Rat olfactory mucosa mesenchymal stem/stromal cells (om-mscs): a characterization study |
title_full_unstemmed |
Rat olfactory mucosa mesenchymal stem/stromal cells (om-mscs): a characterization study |
title_sort |
Rat olfactory mucosa mesenchymal stem/stromal cells (om-mscs): a characterization study |
author |
Alvites, Rui D. |
author_facet |
Alvites, Rui D. Branquinho, Mariana V. Caseiro, Ana R. Amorim, Irina Pedrosa, Sílvia Santos Rêma, Alexandra Faria, Fátima Porto, Beatriz Oliveira, Cláudia Teixeira, Paula Magalhães, Rui Geuna, Stefano Varejão, Artur S. P. Maurício, Ana C. |
author_role |
author |
author2 |
Branquinho, Mariana V. Caseiro, Ana R. Amorim, Irina Pedrosa, Sílvia Santos Rêma, Alexandra Faria, Fátima Porto, Beatriz Oliveira, Cláudia Teixeira, Paula Magalhães, Rui Geuna, Stefano Varejão, Artur S. P. Maurício, Ana C. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Alvites, Rui D. Branquinho, Mariana V. Caseiro, Ana R. Amorim, Irina Pedrosa, Sílvia Santos Rêma, Alexandra Faria, Fátima Porto, Beatriz Oliveira, Cláudia Teixeira, Paula Magalhães, Rui Geuna, Stefano Varejão, Artur S. P. Maurício, Ana C. |
description |
Stem/stromal cell-based therapies are a branch of regenerative medicine and stand as an attractive option to promote the repair of damaged or dysfunctional tissues and organs. Olfactory mucosa mesenchymal stem/stromal cells have been regarded as a promising tool in regenerative therapies because of their several favorable properties such as multipotency, high proliferation rate, helpful location, and few associated ethical issues. These cells are easily accessible in the nasal cavity of most mammals, including the rat, can be easily applied in autologous treatments, and do not cope with most of the obstacles associated with the use of other stem cells. Despite this, its application in preclinical trials and in both human and animal patients is still limited because of the small number of studies performed so far and to the nonexistence of a standard and unambiguous protocol for collection, isolation, and therapeutic application. In the present work a validation of a protocol for isolation, culture, expansion, freezing, and thawing of olfactory mucosa mesenchymal stem/stromal cells was performed, applied to the rat model, as well as a biological characterization of these cells. To investigate the therapeutic potential of OM-MSCs and their eventual safe application in preclinical trials, the main characteristics of OMSC stemness were addressed. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-17T14:04:32Z 2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/30279 |
url |
http://hdl.handle.net/10400.14/30279 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Alvites, R. D., Branquinho, M. V., Caseiro, A. R., Amorim, I., Santos Pedrosa, S., Rêma, A., ..., Magalhães, R. (2020). Rat Olfactory Mucosa Mesenchymal Stem/Stromal Cells (OM-MSCs): A Characterization Study. International Journal of Cell Biology 1687-8876 10.1155/2020/2938258 1687-8884 85079295088 PMC7212324 32411249 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi |
publisher.none.fl_str_mv |
Hindawi |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131955642499072 |