Circulating small non-coding RNAs provide new insights into vitamin K nutrition and reproductive physiology in teleost fish
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/14504 |
Resumo: | Background: Vitamin K (VK) is a fat-soluble vitamin known for its essential role in blood coagulation, but also on other biological processes (e.g. reproduction, brain and bone development) have been recently suggested. Nevertheless, the molecular mechanisms behind its particular function on reproduction are not yet fully understood. Methods: The potential role of VK on reproduction through nutritional supplementation in Senegalese sole (Solea senegalensis) was assessed by gonadal maturation and 11-ketosterone, testosterone and estriol plasma levels when fed with control or VK supplemented (1250 mg kg(-1) of VK,) diets along a six month trial. At the end, sperm production and quality (viability and DNA fragmentation) were evaluated. Circulating small non-coding RNAs (sncRNAs) in blood plasma from males were also studied through RNA-Seq. Results: Fish fed with dietary VK supplementation had increased testosterone levels and lower sperm DNA fragmentation. SncRNAs from blood plasma were found differentially expressed when nutritional and sperm quality conditions were compared. PiR-675//676//4794//5462 and piR-74614 were found up-regulated in males fed with dietary VK supplementation. Let-7g, let-7e(18nt), let-7a-1, let-7a-3//7a-2//7a-1, let-7e(23nt) and piR-675//676//4794//5462 were found to be up-regulated and miR-146a and miR-146a-1//146a-2//146a-3 down-regulated when fish with low and high sperm DNA fragmentation were compared. Bioinformatic analyses of predicted mRNAs targeted by sncRNAs revealed the potential underlying pathways. Conclusions: VK supplementation improves fish gonad maturation and sperm quality, suggesting an unexpected and complex regulation of the nutritional status and reproductive performance through circulating sncRNAs. General significance: The use of circulating sncRNAs as reliable and less-invasive physiological biomarkers in fish nutrition and reproduction has been unveiled. |
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Circulating small non-coding RNAs provide new insights into vitamin K nutrition and reproductive physiology in teleost fishSole solea-senegalensisSex steroid-levelsPlasma-levelsGene-expressionSperm qualityDna-damagePerformanceEnzymeSupplementationMetabolismBackground: Vitamin K (VK) is a fat-soluble vitamin known for its essential role in blood coagulation, but also on other biological processes (e.g. reproduction, brain and bone development) have been recently suggested. Nevertheless, the molecular mechanisms behind its particular function on reproduction are not yet fully understood. Methods: The potential role of VK on reproduction through nutritional supplementation in Senegalese sole (Solea senegalensis) was assessed by gonadal maturation and 11-ketosterone, testosterone and estriol plasma levels when fed with control or VK supplemented (1250 mg kg(-1) of VK,) diets along a six month trial. At the end, sperm production and quality (viability and DNA fragmentation) were evaluated. Circulating small non-coding RNAs (sncRNAs) in blood plasma from males were also studied through RNA-Seq. Results: Fish fed with dietary VK supplementation had increased testosterone levels and lower sperm DNA fragmentation. SncRNAs from blood plasma were found differentially expressed when nutritional and sperm quality conditions were compared. PiR-675//676//4794//5462 and piR-74614 were found up-regulated in males fed with dietary VK supplementation. Let-7g, let-7e(18nt), let-7a-1, let-7a-3//7a-2//7a-1, let-7e(23nt) and piR-675//676//4794//5462 were found to be up-regulated and miR-146a and miR-146a-1//146a-2//146a-3 down-regulated when fish with low and high sperm DNA fragmentation were compared. Bioinformatic analyses of predicted mRNAs targeted by sncRNAs revealed the potential underlying pathways. Conclusions: VK supplementation improves fish gonad maturation and sperm quality, suggesting an unexpected and complex regulation of the nutritional status and reproductive performance through circulating sncRNAs. General significance: The use of circulating sncRNAs as reliable and less-invasive physiological biomarkers in fish nutrition and reproduction has been unveiled.European Regional Development Funds (ERDF) through the Portuguese "Programa Operational" (PROMAR)European Union (EU) [31-03-05-FEP-0073]COST Office (Food and Agriculture COST Action) [FA1205: AQUAGAMETE]network LARVAplus "Estrategias de desarrollo y mejora de la production de larvas de peces en Iberoamerica" - Programa Iberoamericano de Ciencia y Tecnologia para el Desarrollo (CYTED) [117RT0521]CYTED [318RT0549][CCMAR/SC/BPD/09/2016]ElsevierSapientiaI, FernándezFernandes, Jorge M. O.Roberto, VâniaKopp, MartinaOliveira, CatarinaRiesco, Marta F.Dias, JorgeCox, Cymon J.Leonor Cancela, M.Cabrita, ElsaGavaia, Paulo2020-07-24T10:53:32Z2019-012019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/14504eng0304-416510.1016/j.bbagen.2018.09.017info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:26:49Zoai:sapientia.ualg.pt:10400.1/14504Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:05:32.147395Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Circulating small non-coding RNAs provide new insights into vitamin K nutrition and reproductive physiology in teleost fish |
title |
Circulating small non-coding RNAs provide new insights into vitamin K nutrition and reproductive physiology in teleost fish |
spellingShingle |
Circulating small non-coding RNAs provide new insights into vitamin K nutrition and reproductive physiology in teleost fish I, Fernández Sole solea-senegalensis Sex steroid-levels Plasma-levels Gene-expression Sperm quality Dna-damage Performance Enzyme Supplementation Metabolism |
title_short |
Circulating small non-coding RNAs provide new insights into vitamin K nutrition and reproductive physiology in teleost fish |
title_full |
Circulating small non-coding RNAs provide new insights into vitamin K nutrition and reproductive physiology in teleost fish |
title_fullStr |
Circulating small non-coding RNAs provide new insights into vitamin K nutrition and reproductive physiology in teleost fish |
title_full_unstemmed |
Circulating small non-coding RNAs provide new insights into vitamin K nutrition and reproductive physiology in teleost fish |
title_sort |
Circulating small non-coding RNAs provide new insights into vitamin K nutrition and reproductive physiology in teleost fish |
author |
I, Fernández |
author_facet |
I, Fernández Fernandes, Jorge M. O. Roberto, Vânia Kopp, Martina Oliveira, Catarina Riesco, Marta F. Dias, Jorge Cox, Cymon J. Leonor Cancela, M. Cabrita, Elsa Gavaia, Paulo |
author_role |
author |
author2 |
Fernandes, Jorge M. O. Roberto, Vânia Kopp, Martina Oliveira, Catarina Riesco, Marta F. Dias, Jorge Cox, Cymon J. Leonor Cancela, M. Cabrita, Elsa Gavaia, Paulo |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
I, Fernández Fernandes, Jorge M. O. Roberto, Vânia Kopp, Martina Oliveira, Catarina Riesco, Marta F. Dias, Jorge Cox, Cymon J. Leonor Cancela, M. Cabrita, Elsa Gavaia, Paulo |
dc.subject.por.fl_str_mv |
Sole solea-senegalensis Sex steroid-levels Plasma-levels Gene-expression Sperm quality Dna-damage Performance Enzyme Supplementation Metabolism |
topic |
Sole solea-senegalensis Sex steroid-levels Plasma-levels Gene-expression Sperm quality Dna-damage Performance Enzyme Supplementation Metabolism |
description |
Background: Vitamin K (VK) is a fat-soluble vitamin known for its essential role in blood coagulation, but also on other biological processes (e.g. reproduction, brain and bone development) have been recently suggested. Nevertheless, the molecular mechanisms behind its particular function on reproduction are not yet fully understood. Methods: The potential role of VK on reproduction through nutritional supplementation in Senegalese sole (Solea senegalensis) was assessed by gonadal maturation and 11-ketosterone, testosterone and estriol plasma levels when fed with control or VK supplemented (1250 mg kg(-1) of VK,) diets along a six month trial. At the end, sperm production and quality (viability and DNA fragmentation) were evaluated. Circulating small non-coding RNAs (sncRNAs) in blood plasma from males were also studied through RNA-Seq. Results: Fish fed with dietary VK supplementation had increased testosterone levels and lower sperm DNA fragmentation. SncRNAs from blood plasma were found differentially expressed when nutritional and sperm quality conditions were compared. PiR-675//676//4794//5462 and piR-74614 were found up-regulated in males fed with dietary VK supplementation. Let-7g, let-7e(18nt), let-7a-1, let-7a-3//7a-2//7a-1, let-7e(23nt) and piR-675//676//4794//5462 were found to be up-regulated and miR-146a and miR-146a-1//146a-2//146a-3 down-regulated when fish with low and high sperm DNA fragmentation were compared. Bioinformatic analyses of predicted mRNAs targeted by sncRNAs revealed the potential underlying pathways. Conclusions: VK supplementation improves fish gonad maturation and sperm quality, suggesting an unexpected and complex regulation of the nutritional status and reproductive performance through circulating sncRNAs. General significance: The use of circulating sncRNAs as reliable and less-invasive physiological biomarkers in fish nutrition and reproduction has been unveiled. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01 2019-01-01T00:00:00Z 2020-07-24T10:53:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/14504 |
url |
http://hdl.handle.net/10400.1/14504 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0304-4165 10.1016/j.bbagen.2018.09.017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133295151153152 |