Bark extract of Uncaria tomentosa L. for the control of strawberry phytopathogens
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/80599 |
Resumo: | Gray mold (<i>Botrytis cinerea</i> Pers.), crown and fruit rot (<i>Phytophthora cactorum</i> (Lebert and Cohn) J.Schröt), and verticillium wilt (<i>Verticillium dahliae</i> Kleb.) are among the main diseases that affect the strawberry crop. In the study presented herein, the bark extract of <i>Uncaria tomentosa</i> (Willd. ex Schult.) DC, popularly known as “cat’s claw”, has been evaluated for its capability to act as a sustainable control method. The bioactive compounds present in the aqueous ammonia extract were characterized by gas chromatography–mass spectroscopy, and the antimicrobial activity of the extract—alone and in combination with chitosan oligomers (COS)—was assessed in vitro and as a coating for postharvest treatment during storage. Octyl isobutyrate (30.7%), 19α methyl-2-oxoformosanan-16-carboxylate (9.3%), tetrahydro-2-methyl-thiophene (4.7%), and α-methyl manofuranoside (4.4%) were identified as the main phytoconstituents. The results of in vitro growth inhibition tests showed that, upon conjugation of the bark extract with COS, complete inhibition was reached at concentrations in the 39–93.75 μg∙mL<sup>−1</sup> range, depending on the pathogen. Concerning the effect of the treatment as a coating to prolong the storage life and control decay during post-harvest storage, high protection was observed at a concentration of 1000 μg∙mL<sup>−1</sup>. Because of this effectiveness, higher than that attained with conventional synthetic fungicides, the bark extracts of cat’s claw may hold promise for strawberry crop protection. |
id |
RCAP_8328a28f1dab04781c5a53bd6ff918db |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/80599 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Bark extract of Uncaria tomentosa L. for the control of strawberry phytopathogensCat’s clawChitosan oligomersCoatingCrown rotGC–MSGray moldLeather rotPostharvestVerticillium wiltScience & TechnologyGray mold (<i>Botrytis cinerea</i> Pers.), crown and fruit rot (<i>Phytophthora cactorum</i> (Lebert and Cohn) J.Schröt), and verticillium wilt (<i>Verticillium dahliae</i> Kleb.) are among the main diseases that affect the strawberry crop. In the study presented herein, the bark extract of <i>Uncaria tomentosa</i> (Willd. ex Schult.) DC, popularly known as “cat’s claw”, has been evaluated for its capability to act as a sustainable control method. The bioactive compounds present in the aqueous ammonia extract were characterized by gas chromatography–mass spectroscopy, and the antimicrobial activity of the extract—alone and in combination with chitosan oligomers (COS)—was assessed in vitro and as a coating for postharvest treatment during storage. Octyl isobutyrate (30.7%), 19α methyl-2-oxoformosanan-16-carboxylate (9.3%), tetrahydro-2-methyl-thiophene (4.7%), and α-methyl manofuranoside (4.4%) were identified as the main phytoconstituents. The results of in vitro growth inhibition tests showed that, upon conjugation of the bark extract with COS, complete inhibition was reached at concentrations in the 39–93.75 μg∙mL<sup>−1</sup> range, depending on the pathogen. Concerning the effect of the treatment as a coating to prolong the storage life and control decay during post-harvest storage, high protection was observed at a concentration of 1000 μg∙mL<sup>−1</sup>. Because of this effectiveness, higher than that attained with conventional synthetic fungicides, the bark extracts of cat’s claw may hold promise for strawberry crop protection.This research was funded by Junta de Castilla y León under project VA258P18, with FEDER co-funding. This work was supported by the “Contrato-Programa” UIDB/04050/2020 funded by Portuguese national funds through the FCT I.P. and by AgrifoodXXI (NORTE-01-0145-FEDER-000041).Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoSánchez-Hernández, EvaMartín-Ramos, PabloMartín-Gil, JesúsSantiago-Aliste, AlbertoHernández-Navarro, SalvadorOliveira, Rui Pedro Soares deGonzález-García, Vicente2022-07-232022-07-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/80599engSánchez-Hernández, E.; Martín-Ramos, P.; Martín-Gil, J.; Santiago-Aliste, A.; Hernández-Navarro, S.; Oliveira, R.; González-García, V. Bark Extract of Uncaria tomentosa L. for the Control of Strawberry Phytopathogens. Horticulturae 2022, 8, 672. https://doi.org/10.3390/horticulturae80806722311-752410.3390/horticulturae8080672672https://www.mdpi.com/2311-7524/8/8/672info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:07:27Zoai:repositorium.sdum.uminho.pt:1822/80599Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:58:26.868150Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bark extract of Uncaria tomentosa L. for the control of strawberry phytopathogens |
title |
Bark extract of Uncaria tomentosa L. for the control of strawberry phytopathogens |
spellingShingle |
Bark extract of Uncaria tomentosa L. for the control of strawberry phytopathogens Sánchez-Hernández, Eva Cat’s claw Chitosan oligomers Coating Crown rot GC–MS Gray mold Leather rot Postharvest Verticillium wilt Science & Technology |
title_short |
Bark extract of Uncaria tomentosa L. for the control of strawberry phytopathogens |
title_full |
Bark extract of Uncaria tomentosa L. for the control of strawberry phytopathogens |
title_fullStr |
Bark extract of Uncaria tomentosa L. for the control of strawberry phytopathogens |
title_full_unstemmed |
Bark extract of Uncaria tomentosa L. for the control of strawberry phytopathogens |
title_sort |
Bark extract of Uncaria tomentosa L. for the control of strawberry phytopathogens |
author |
Sánchez-Hernández, Eva |
author_facet |
Sánchez-Hernández, Eva Martín-Ramos, Pablo Martín-Gil, Jesús Santiago-Aliste, Alberto Hernández-Navarro, Salvador Oliveira, Rui Pedro Soares de González-García, Vicente |
author_role |
author |
author2 |
Martín-Ramos, Pablo Martín-Gil, Jesús Santiago-Aliste, Alberto Hernández-Navarro, Salvador Oliveira, Rui Pedro Soares de González-García, Vicente |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Sánchez-Hernández, Eva Martín-Ramos, Pablo Martín-Gil, Jesús Santiago-Aliste, Alberto Hernández-Navarro, Salvador Oliveira, Rui Pedro Soares de González-García, Vicente |
dc.subject.por.fl_str_mv |
Cat’s claw Chitosan oligomers Coating Crown rot GC–MS Gray mold Leather rot Postharvest Verticillium wilt Science & Technology |
topic |
Cat’s claw Chitosan oligomers Coating Crown rot GC–MS Gray mold Leather rot Postharvest Verticillium wilt Science & Technology |
description |
Gray mold (<i>Botrytis cinerea</i> Pers.), crown and fruit rot (<i>Phytophthora cactorum</i> (Lebert and Cohn) J.Schröt), and verticillium wilt (<i>Verticillium dahliae</i> Kleb.) are among the main diseases that affect the strawberry crop. In the study presented herein, the bark extract of <i>Uncaria tomentosa</i> (Willd. ex Schult.) DC, popularly known as “cat’s claw”, has been evaluated for its capability to act as a sustainable control method. The bioactive compounds present in the aqueous ammonia extract were characterized by gas chromatography–mass spectroscopy, and the antimicrobial activity of the extract—alone and in combination with chitosan oligomers (COS)—was assessed in vitro and as a coating for postharvest treatment during storage. Octyl isobutyrate (30.7%), 19α methyl-2-oxoformosanan-16-carboxylate (9.3%), tetrahydro-2-methyl-thiophene (4.7%), and α-methyl manofuranoside (4.4%) were identified as the main phytoconstituents. The results of in vitro growth inhibition tests showed that, upon conjugation of the bark extract with COS, complete inhibition was reached at concentrations in the 39–93.75 μg∙mL<sup>−1</sup> range, depending on the pathogen. Concerning the effect of the treatment as a coating to prolong the storage life and control decay during post-harvest storage, high protection was observed at a concentration of 1000 μg∙mL<sup>−1</sup>. Because of this effectiveness, higher than that attained with conventional synthetic fungicides, the bark extracts of cat’s claw may hold promise for strawberry crop protection. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-23 2022-07-23T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/80599 |
url |
https://hdl.handle.net/1822/80599 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Sánchez-Hernández, E.; Martín-Ramos, P.; Martín-Gil, J.; Santiago-Aliste, A.; Hernández-Navarro, S.; Oliveira, R.; González-García, V. Bark Extract of Uncaria tomentosa L. for the Control of Strawberry Phytopathogens. Horticulturae 2022, 8, 672. https://doi.org/10.3390/horticulturae8080672 2311-7524 10.3390/horticulturae8080672 672 https://www.mdpi.com/2311-7524/8/8/672 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132374734209024 |