Staphylococcus epidermidis biofilms control by N-acetylcysteine and rifampicin
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/27801 |
Resumo: | Medical device-associated infections caused by Staphylococcus epidermidis usually involve biofilm formation and its eradication is particularly challenging. Although rifampicin has been proving to be one of the most effective antibiotics against S. epidermidis biofilms, its use as a single agent can lead to the acquisition of resistance. Therefore, we assessed the combined effect of rifampicin with N-acetylcysteine (NAC) known by its mucolytic effect, in the control of S. epidermidis biofilms. Biofilms of 2 S. epidermidis strains (9142 and 1457) were treated with 1x minimum inhibitory concentration (4 mg/mL) and 10x minimum inhibitory concentration (40 mg/mL) of NAC and 10 mg/L (peak serum) of rifampicin alone and in combination. NAC at 40 mg/L alone or in combination with rifampicin (10 mg/L) significantly reduced (4 log10) the number of biofilm cells. Considering their different modes of action, the association of NAC with rifampicin constitutes a promising therapeutic strategy in the treatment of infections associated to S. epidermidis biofilms. |
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Staphylococcus epidermidis biofilms control by N-acetylcysteine and rifampicinbiofilmN-acetylcysteineplanktonic cellsrifampicinStaphylococcus epidermidisScience & TechnologyMedical device-associated infections caused by Staphylococcus epidermidis usually involve biofilm formation and its eradication is particularly challenging. Although rifampicin has been proving to be one of the most effective antibiotics against S. epidermidis biofilms, its use as a single agent can lead to the acquisition of resistance. Therefore, we assessed the combined effect of rifampicin with N-acetylcysteine (NAC) known by its mucolytic effect, in the control of S. epidermidis biofilms. Biofilms of 2 S. epidermidis strains (9142 and 1457) were treated with 1x minimum inhibitory concentration (4 mg/mL) and 10x minimum inhibitory concentration (40 mg/mL) of NAC and 10 mg/L (peak serum) of rifampicin alone and in combination. NAC at 40 mg/L alone or in combination with rifampicin (10 mg/L) significantly reduced (4 log10) the number of biofilm cells. Considering their different modes of action, the association of NAC with rifampicin constitutes a promising therapeutic strategy in the treatment of infections associated to S. epidermidis biofilms.Bruna Leite acknowledges the financial support of ISAC/Program Erasmus Munds External Cooperation and the IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus of Gualtar.Lippincott, Williams & WilkinsLippincott Williams & Wilkins Ltd.Universidade do MinhoLeite, BrunaGomes, F. I.Teixeira, P.Souza, ClovisPizzolitto, ElisabethOliveira, Rosário20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/27801eng1536-36861075-276510.1097/MJT.0b013e318209e17binfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:54:45Zoai:repositorium.sdum.uminho.pt:1822/27801Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:54:45Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Staphylococcus epidermidis biofilms control by N-acetylcysteine and rifampicin |
title |
Staphylococcus epidermidis biofilms control by N-acetylcysteine and rifampicin |
spellingShingle |
Staphylococcus epidermidis biofilms control by N-acetylcysteine and rifampicin Leite, Bruna biofilm N-acetylcysteine planktonic cells rifampicin Staphylococcus epidermidis Science & Technology |
title_short |
Staphylococcus epidermidis biofilms control by N-acetylcysteine and rifampicin |
title_full |
Staphylococcus epidermidis biofilms control by N-acetylcysteine and rifampicin |
title_fullStr |
Staphylococcus epidermidis biofilms control by N-acetylcysteine and rifampicin |
title_full_unstemmed |
Staphylococcus epidermidis biofilms control by N-acetylcysteine and rifampicin |
title_sort |
Staphylococcus epidermidis biofilms control by N-acetylcysteine and rifampicin |
author |
Leite, Bruna |
author_facet |
Leite, Bruna Gomes, F. I. Teixeira, P. Souza, Clovis Pizzolitto, Elisabeth Oliveira, Rosário |
author_role |
author |
author2 |
Gomes, F. I. Teixeira, P. Souza, Clovis Pizzolitto, Elisabeth Oliveira, Rosário |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Leite, Bruna Gomes, F. I. Teixeira, P. Souza, Clovis Pizzolitto, Elisabeth Oliveira, Rosário |
dc.subject.por.fl_str_mv |
biofilm N-acetylcysteine planktonic cells rifampicin Staphylococcus epidermidis Science & Technology |
topic |
biofilm N-acetylcysteine planktonic cells rifampicin Staphylococcus epidermidis Science & Technology |
description |
Medical device-associated infections caused by Staphylococcus epidermidis usually involve biofilm formation and its eradication is particularly challenging. Although rifampicin has been proving to be one of the most effective antibiotics against S. epidermidis biofilms, its use as a single agent can lead to the acquisition of resistance. Therefore, we assessed the combined effect of rifampicin with N-acetylcysteine (NAC) known by its mucolytic effect, in the control of S. epidermidis biofilms. Biofilms of 2 S. epidermidis strains (9142 and 1457) were treated with 1x minimum inhibitory concentration (4 mg/mL) and 10x minimum inhibitory concentration (40 mg/mL) of NAC and 10 mg/L (peak serum) of rifampicin alone and in combination. NAC at 40 mg/L alone or in combination with rifampicin (10 mg/L) significantly reduced (4 log10) the number of biofilm cells. Considering their different modes of action, the association of NAC with rifampicin constitutes a promising therapeutic strategy in the treatment of infections associated to S. epidermidis biofilms. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/27801 |
url |
http://hdl.handle.net/1822/27801 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1536-3686 1075-2765 10.1097/MJT.0b013e318209e17b |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Lippincott, Williams & Wilkins Lippincott Williams & Wilkins Ltd. |
publisher.none.fl_str_mv |
Lippincott, Williams & Wilkins Lippincott Williams & Wilkins Ltd. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817545135612755968 |