The Hypocholesterolemic Potential of the Edible Algae Fucus vesiculosus

Detalhes bibliográficos
Autor(a) principal: André, Rebeca
Data de Publicação: 2023
Outros Autores: Pacheco, Rita, Alves, Ana Catarina, Santos, Hugo M., Bourbon, Mafalda, Serralheiro, Maria Luísa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/159763
Resumo: PM003/2016). Publisher Copyright: © 2023 by the authors.
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spelling The Hypocholesterolemic Potential of the Edible Algae Fucus vesiculosusProteomic and Quantitative PCR AnalysisABCG5cholesterol excretioncholesterol synthesisezetimibeFucus vesiculosusNPC1L1Food ScienceMicrobiologyHealth(social science)Health Professions (miscellaneous)Plant SciencePM003/2016). Publisher Copyright: © 2023 by the authors.A brown seaweed consumed worldwide, Fucus vesiculosus, has been used to prevent atherosclerosis and hypercholesterolemia, among other uses. However, the mechanisms of action that lead to these effects are not yet fully understood. This work aims to study the in vitro effect of an aqueous extract of F. vesiculosus, previously characterized as rich in phlorotannins and peptides, on the expression of different proteins involved in the synthesis and transport of cholesterol. A proteomic analysis, Western blot, and qRT-PCR analysis were performed to identify protein changes in HepG2 cells exposed to 0.25 mg/mL of the F. vesiculosus extract for 24 h. The proteomic results demonstrated that, in liver cells, the extract decreases the expression of four proteins involved in the cholesterol biosynthesis process (CYP51A1, DHCR24, HMGCS1 and HSD17B7). Additionally, a 12.76% and 18.40% decrease in the expression of two important transporters proteins of cholesterol, NPC1L1 and ABCG5, respectively, was also observed, as well as a 30% decrease in NPC1L1 mRNA levels in the cells exposed to the extract compared to control cells. Our study reveals some of the mechanisms underlying the actions of bioactive compounds from F. vesiculosus that may explain its previously reported hypocholesterolemic effect, future prospecting its use as a functional food.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNAndré, RebecaPacheco, RitaAlves, Ana CatarinaSantos, Hugo M.Bourbon, MafaldaSerralheiro, Maria Luísa2023-11-09T22:09:13Z2023-07-202023-07-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttp://hdl.handle.net/10362/159763engAndré, R., Pacheco, R., Alves, A. C., Santos, H. M., Bourbon, M., & Serralheiro, M. L. (2023). The Hypocholesterolemic Potential of the Edible Algae Fucus vesiculosus: Proteomic and Quantitative PCR Analysis. Foods, 12(14), [2758]. https://doi.org/10.3390/foods121427582304-8158PURE: 75756606https://doi.org/10.3390/foods12142758info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:42:14Zoai:run.unl.pt:10362/159763Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:41.798187Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The Hypocholesterolemic Potential of the Edible Algae Fucus vesiculosus
Proteomic and Quantitative PCR Analysis
title The Hypocholesterolemic Potential of the Edible Algae Fucus vesiculosus
spellingShingle The Hypocholesterolemic Potential of the Edible Algae Fucus vesiculosus
André, Rebeca
ABCG5
cholesterol excretion
cholesterol synthesis
ezetimibe
Fucus vesiculosus
NPC1L1
Food Science
Microbiology
Health(social science)
Health Professions (miscellaneous)
Plant Science
title_short The Hypocholesterolemic Potential of the Edible Algae Fucus vesiculosus
title_full The Hypocholesterolemic Potential of the Edible Algae Fucus vesiculosus
title_fullStr The Hypocholesterolemic Potential of the Edible Algae Fucus vesiculosus
title_full_unstemmed The Hypocholesterolemic Potential of the Edible Algae Fucus vesiculosus
title_sort The Hypocholesterolemic Potential of the Edible Algae Fucus vesiculosus
author André, Rebeca
author_facet André, Rebeca
Pacheco, Rita
Alves, Ana Catarina
Santos, Hugo M.
Bourbon, Mafalda
Serralheiro, Maria Luísa
author_role author
author2 Pacheco, Rita
Alves, Ana Catarina
Santos, Hugo M.
Bourbon, Mafalda
Serralheiro, Maria Luísa
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv André, Rebeca
Pacheco, Rita
Alves, Ana Catarina
Santos, Hugo M.
Bourbon, Mafalda
Serralheiro, Maria Luísa
dc.subject.por.fl_str_mv ABCG5
cholesterol excretion
cholesterol synthesis
ezetimibe
Fucus vesiculosus
NPC1L1
Food Science
Microbiology
Health(social science)
Health Professions (miscellaneous)
Plant Science
topic ABCG5
cholesterol excretion
cholesterol synthesis
ezetimibe
Fucus vesiculosus
NPC1L1
Food Science
Microbiology
Health(social science)
Health Professions (miscellaneous)
Plant Science
description PM003/2016). Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-11-09T22:09:13Z
2023-07-20
2023-07-20T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/159763
url http://hdl.handle.net/10362/159763
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv André, R., Pacheco, R., Alves, A. C., Santos, H. M., Bourbon, M., & Serralheiro, M. L. (2023). The Hypocholesterolemic Potential of the Edible Algae Fucus vesiculosus: Proteomic and Quantitative PCR Analysis. Foods, 12(14), [2758]. https://doi.org/10.3390/foods12142758
2304-8158
PURE: 75756606
https://doi.org/10.3390/foods12142758
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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