Plant defensin PvD1 modulates the membrane composition of breast tumour-derived exosomes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/57218 |
Resumo: | This journal is © The Royal Society of Chemistry 2019 |
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Plant defensin PvD1 modulates the membrane composition of breast tumour-derived exosomesThis journal is © The Royal Society of Chemistry 2019One of the most important causes of failure in tumour treatment is the development of resistance to therapy. Cancer cells can develop the ability to lose sensitivity to anti-neoplastic drugs during reciprocal crosstalk between cells and their interaction with the tumour microenvironment (TME). Cell-to-cell communication regulates a cascade of interdependent events essential for disease development and progression and can be mediated by several signalling pathways. Exosome-mediated communication is one of the pathways regulating these events. Tumour-derived exosomes (TDE) are believed to have the ability to modulate TMEs and participate in multidrug resistance mechanisms. In this work, we studied the effect of the natural defensin from common bean, PvD1, on the formation of exosomes by breast cancer MCF-7 cells, mainly the modulatory effect it has on the level of CD63 and CD9 tetraspanins. Moreover, we followed the interaction of PvD1 with biological and model membranes of selected composition, by biophysical and imaging techniques. Overall, the results show that PvD1 induces a dual effect on MCF-7 derived exosomes: the peptide attenuates the recruitment of CD63 and CD9 to exosomes intracellularly and binds to the mature exosomes in the extracellular environment. This work uncovers the exosomemediated anticancer action of PvD1, a potential nutraceutical agent.The authors thank Fundação para a Ciência e a Tecnologia (FCT I.P., Portugal) for funding – PTDC/BBB-BQB/1693/2014, and also acknowledge financial support from the Brazilian agencies CNPq, CAPES, and FAPERJ (E-26/203.090/2016; E-26/202.132/2015). Julia Skalska, Filipa D. Oliveira, Tiago N. Figueira and Diana Gaspar acknowledge FCT I.P. for fellowships PD/BD/114177/2016, PD/BD/135046/2017, SFRH/BD/5283/2013 and SFRH/BPD/109010/2015 respectively. Marie Skłodowska-Curie Research and Innovation Staff Exchange (RISE) is also acknowledged for funding: call H2020-MSCA-RISE-2014, Grant agreement 644167, 2015–2019.Royal Society of ChemistryRepositório da Universidade de LisboaSkalska, JuliaOliveira, FilipaFigueira, Tiago N.Mello, Érica O.Gomes, Valdirene M.McNaughton-Smith, GrantCastanho, Miguel A. R. B.Gaspar, Diana2023-04-21T11:41:24Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/57218engNanoscale, 2019, 11, 23366–233812040-336410.1039/C9NR07843F2040-3372info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:05:18Zoai:repositorio.ul.pt:10451/57218Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:07:39.042139Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Plant defensin PvD1 modulates the membrane composition of breast tumour-derived exosomes |
| title |
Plant defensin PvD1 modulates the membrane composition of breast tumour-derived exosomes |
| spellingShingle |
Plant defensin PvD1 modulates the membrane composition of breast tumour-derived exosomes Skalska, Julia |
| title_short |
Plant defensin PvD1 modulates the membrane composition of breast tumour-derived exosomes |
| title_full |
Plant defensin PvD1 modulates the membrane composition of breast tumour-derived exosomes |
| title_fullStr |
Plant defensin PvD1 modulates the membrane composition of breast tumour-derived exosomes |
| title_full_unstemmed |
Plant defensin PvD1 modulates the membrane composition of breast tumour-derived exosomes |
| title_sort |
Plant defensin PvD1 modulates the membrane composition of breast tumour-derived exosomes |
| author |
Skalska, Julia |
| author_facet |
Skalska, Julia Oliveira, Filipa Figueira, Tiago N. Mello, Érica O. Gomes, Valdirene M. McNaughton-Smith, Grant Castanho, Miguel A. R. B. Gaspar, Diana |
| author_role |
author |
| author2 |
Oliveira, Filipa Figueira, Tiago N. Mello, Érica O. Gomes, Valdirene M. McNaughton-Smith, Grant Castanho, Miguel A. R. B. Gaspar, Diana |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Skalska, Julia Oliveira, Filipa Figueira, Tiago N. Mello, Érica O. Gomes, Valdirene M. McNaughton-Smith, Grant Castanho, Miguel A. R. B. Gaspar, Diana |
| description |
This journal is © The Royal Society of Chemistry 2019 |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019 2019-01-01T00:00:00Z 2023-04-21T11:41:24Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/57218 |
| url |
http://hdl.handle.net/10451/57218 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Nanoscale, 2019, 11, 23366–23381 2040-3364 10.1039/C9NR07843F 2040-3372 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
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Royal Society of Chemistry |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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