Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/82036 |
Resumo: | Electronic cigarette usage has significantly expanded among young people and pregnant women in the last decade. Although there are already some data regarding the short- and long-term consequences of e-cigarettes on human health, their effect on embryo and lung development still needs to be fully disclosed. In this sense, this study describes, for the first time, the impact of electronic cigarette aerosol on early lung development. For this purpose, ex vivo chick (Gallus gallus) embryonic lungs were cultured in vitro for 48h in e-cigarette aerosol exposed-medium or unexposed medium. Chick lung explants were also cultured in a cigarette smoke-exposed medium for comparison purposes. Lung explants were morphologically analyzed to assess the impact on lung growth. Additionally, TNF- levels were determined in the supernatant as a marker of pro-inflammatory response. The results suggest that electronic cigarette aerosol impairs lung growth and promotes lung inflammation. However, its impact on early lung growth seems less detrimental than conventional cigarette smoke. This work provides significant data regarding the impact of e-cig aerosol, adding to the efforts to fully understand its effect on embryo development. The validation of these effects may eventually lead to new tobacco control recommendations for pregnant women. |
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Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explantsSmokingVapingLung DevelopmentBranching MorphogenesisInflammationScience & TechnologyElectronic cigarette usage has significantly expanded among young people and pregnant women in the last decade. Although there are already some data regarding the short- and long-term consequences of e-cigarettes on human health, their effect on embryo and lung development still needs to be fully disclosed. In this sense, this study describes, for the first time, the impact of electronic cigarette aerosol on early lung development. For this purpose, ex vivo chick (Gallus gallus) embryonic lungs were cultured in vitro for 48h in e-cigarette aerosol exposed-medium or unexposed medium. Chick lung explants were also cultured in a cigarette smoke-exposed medium for comparison purposes. Lung explants were morphologically analyzed to assess the impact on lung growth. Additionally, TNF- levels were determined in the supernatant as a marker of pro-inflammatory response. The results suggest that electronic cigarette aerosol impairs lung growth and promotes lung inflammation. However, its impact on early lung growth seems less detrimental than conventional cigarette smoke. This work provides significant data regarding the impact of e-cig aerosol, adding to the efforts to fully understand its effect on embryo development. The validation of these effects may eventually lead to new tobacco control recommendations for pregnant women.This work has been funded by National funds, through the Foundation for Science and Technology (FCT) - project UIDB/50026/2020, UIDP/50026/2020, UIDB/04469/2020 and UIDB/04050/2020. Funding bodies did not play any role in the study design, data collection, and analysis, publication decisions or preparation of the manuscript.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoSilva-Ribeiro, TiagoCoelho, Eduardo João LouroGenisheva, ZlatinaOliveira, José MariaCorreia-Pinto, JorgeSampaio, PaulaMoura, Rute S.2023-01-112023-01-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/82036engSilva-Ribeiro, T., Coelho, E., Genisheva, Z., Oliveira, J. M., Correia-Pinto, J., Sampaio, P., & Moura, R. S. (2023, March). Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants. Toxicology Letters. Elsevier BV. http://doi.org/10.1016/j.toxlet.2023.01.0020378-427410.1016/j.toxlet.2023.01.00236638931https://www.sciencedirect.com/science/article/pii/S0378427423000085info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-23T01:31:31Zoai:repositorium.sdum.uminho.pt:1822/82036Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:20:58.747685Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants |
title |
Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants |
spellingShingle |
Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants Silva-Ribeiro, Tiago Smoking Vaping Lung Development Branching Morphogenesis Inflammation Science & Technology |
title_short |
Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants |
title_full |
Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants |
title_fullStr |
Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants |
title_full_unstemmed |
Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants |
title_sort |
Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants |
author |
Silva-Ribeiro, Tiago |
author_facet |
Silva-Ribeiro, Tiago Coelho, Eduardo João Louro Genisheva, Zlatina Oliveira, José Maria Correia-Pinto, Jorge Sampaio, Paula Moura, Rute S. |
author_role |
author |
author2 |
Coelho, Eduardo João Louro Genisheva, Zlatina Oliveira, José Maria Correia-Pinto, Jorge Sampaio, Paula Moura, Rute S. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva-Ribeiro, Tiago Coelho, Eduardo João Louro Genisheva, Zlatina Oliveira, José Maria Correia-Pinto, Jorge Sampaio, Paula Moura, Rute S. |
dc.subject.por.fl_str_mv |
Smoking Vaping Lung Development Branching Morphogenesis Inflammation Science & Technology |
topic |
Smoking Vaping Lung Development Branching Morphogenesis Inflammation Science & Technology |
description |
Electronic cigarette usage has significantly expanded among young people and pregnant women in the last decade. Although there are already some data regarding the short- and long-term consequences of e-cigarettes on human health, their effect on embryo and lung development still needs to be fully disclosed. In this sense, this study describes, for the first time, the impact of electronic cigarette aerosol on early lung development. For this purpose, ex vivo chick (Gallus gallus) embryonic lungs were cultured in vitro for 48h in e-cigarette aerosol exposed-medium or unexposed medium. Chick lung explants were also cultured in a cigarette smoke-exposed medium for comparison purposes. Lung explants were morphologically analyzed to assess the impact on lung growth. Additionally, TNF- levels were determined in the supernatant as a marker of pro-inflammatory response. The results suggest that electronic cigarette aerosol impairs lung growth and promotes lung inflammation. However, its impact on early lung growth seems less detrimental than conventional cigarette smoke. This work provides significant data regarding the impact of e-cig aerosol, adding to the efforts to fully understand its effect on embryo development. The validation of these effects may eventually lead to new tobacco control recommendations for pregnant women. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-01-11 2023-01-11T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/82036 |
url |
https://hdl.handle.net/1822/82036 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva-Ribeiro, T., Coelho, E., Genisheva, Z., Oliveira, J. M., Correia-Pinto, J., Sampaio, P., & Moura, R. S. (2023, March). Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants. Toxicology Letters. Elsevier BV. http://doi.org/10.1016/j.toxlet.2023.01.002 0378-4274 10.1016/j.toxlet.2023.01.002 36638931 https://www.sciencedirect.com/science/article/pii/S0378427423000085 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132675100901376 |