Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants

Detalhes bibliográficos
Autor(a) principal: Silva-Ribeiro, Tiago
Data de Publicação: 2023
Outros Autores: Coelho, Eduardo João Louro, Genisheva, Zlatina, Oliveira, José Maria, Correia-Pinto, Jorge, Sampaio, Paula, Moura, Rute S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/82036
Resumo: Electronic cigarette usage has significantly expanded among young people and pregnant women in the last decade. Although there are already some data regarding the short- and long-term consequences of e-cigarettes on human health, their effect on embryo and lung development still needs to be fully disclosed. In this sense, this study describes, for the first time, the impact of electronic cigarette aerosol on early lung development. For this purpose, ex vivo chick (Gallus gallus) embryonic lungs were cultured in vitro for 48h in e-cigarette aerosol exposed-medium or unexposed medium. Chick lung explants were also cultured in a cigarette smoke-exposed medium for comparison purposes. Lung explants were morphologically analyzed to assess the impact on lung growth. Additionally, TNF- levels were determined in the supernatant as a marker of pro-inflammatory response. The results suggest that electronic cigarette aerosol impairs lung growth and promotes lung inflammation. However, its impact on early lung growth seems less detrimental than conventional cigarette smoke. This work provides significant data regarding the impact of e-cig aerosol, adding to the efforts to fully understand its effect on embryo development. The validation of these effects may eventually lead to new tobacco control recommendations for pregnant women.
id RCAP_84ad73bb7ee6c35beafd5000e7e1c0e4
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/82036
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explantsSmokingVapingLung DevelopmentBranching MorphogenesisInflammationScience & TechnologyElectronic cigarette usage has significantly expanded among young people and pregnant women in the last decade. Although there are already some data regarding the short- and long-term consequences of e-cigarettes on human health, their effect on embryo and lung development still needs to be fully disclosed. In this sense, this study describes, for the first time, the impact of electronic cigarette aerosol on early lung development. For this purpose, ex vivo chick (Gallus gallus) embryonic lungs were cultured in vitro for 48h in e-cigarette aerosol exposed-medium or unexposed medium. Chick lung explants were also cultured in a cigarette smoke-exposed medium for comparison purposes. Lung explants were morphologically analyzed to assess the impact on lung growth. Additionally, TNF- levels were determined in the supernatant as a marker of pro-inflammatory response. The results suggest that electronic cigarette aerosol impairs lung growth and promotes lung inflammation. However, its impact on early lung growth seems less detrimental than conventional cigarette smoke. This work provides significant data regarding the impact of e-cig aerosol, adding to the efforts to fully understand its effect on embryo development. The validation of these effects may eventually lead to new tobacco control recommendations for pregnant women.This work has been funded by National funds, through the Foundation for Science and Technology (FCT) - project UIDB/50026/2020, UIDP/50026/2020, UIDB/04469/2020 and UIDB/04050/2020. Funding bodies did not play any role in the study design, data collection, and analysis, publication decisions or preparation of the manuscript.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoSilva-Ribeiro, TiagoCoelho, Eduardo João LouroGenisheva, ZlatinaOliveira, José MariaCorreia-Pinto, JorgeSampaio, PaulaMoura, Rute S.2023-01-112023-01-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/82036engSilva-Ribeiro, T., Coelho, E., Genisheva, Z., Oliveira, J. M., Correia-Pinto, J., Sampaio, P., & Moura, R. S. (2023, March). Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants. Toxicology Letters. Elsevier BV. http://doi.org/10.1016/j.toxlet.2023.01.0020378-427410.1016/j.toxlet.2023.01.00236638931https://www.sciencedirect.com/science/article/pii/S0378427423000085info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-23T01:31:31Zoai:repositorium.sdum.uminho.pt:1822/82036Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:20:58.747685Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants
title Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants
spellingShingle Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants
Silva-Ribeiro, Tiago
Smoking
Vaping
Lung Development
Branching Morphogenesis
Inflammation
Science & Technology
title_short Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants
title_full Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants
title_fullStr Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants
title_full_unstemmed Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants
title_sort Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants
author Silva-Ribeiro, Tiago
author_facet Silva-Ribeiro, Tiago
Coelho, Eduardo João Louro
Genisheva, Zlatina
Oliveira, José Maria
Correia-Pinto, Jorge
Sampaio, Paula
Moura, Rute S.
author_role author
author2 Coelho, Eduardo João Louro
Genisheva, Zlatina
Oliveira, José Maria
Correia-Pinto, Jorge
Sampaio, Paula
Moura, Rute S.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva-Ribeiro, Tiago
Coelho, Eduardo João Louro
Genisheva, Zlatina
Oliveira, José Maria
Correia-Pinto, Jorge
Sampaio, Paula
Moura, Rute S.
dc.subject.por.fl_str_mv Smoking
Vaping
Lung Development
Branching Morphogenesis
Inflammation
Science & Technology
topic Smoking
Vaping
Lung Development
Branching Morphogenesis
Inflammation
Science & Technology
description Electronic cigarette usage has significantly expanded among young people and pregnant women in the last decade. Although there are already some data regarding the short- and long-term consequences of e-cigarettes on human health, their effect on embryo and lung development still needs to be fully disclosed. In this sense, this study describes, for the first time, the impact of electronic cigarette aerosol on early lung development. For this purpose, ex vivo chick (Gallus gallus) embryonic lungs were cultured in vitro for 48h in e-cigarette aerosol exposed-medium or unexposed medium. Chick lung explants were also cultured in a cigarette smoke-exposed medium for comparison purposes. Lung explants were morphologically analyzed to assess the impact on lung growth. Additionally, TNF- levels were determined in the supernatant as a marker of pro-inflammatory response. The results suggest that electronic cigarette aerosol impairs lung growth and promotes lung inflammation. However, its impact on early lung growth seems less detrimental than conventional cigarette smoke. This work provides significant data regarding the impact of e-cig aerosol, adding to the efforts to fully understand its effect on embryo development. The validation of these effects may eventually lead to new tobacco control recommendations for pregnant women.
publishDate 2023
dc.date.none.fl_str_mv 2023-01-11
2023-01-11T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/82036
url https://hdl.handle.net/1822/82036
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva-Ribeiro, T., Coelho, E., Genisheva, Z., Oliveira, J. M., Correia-Pinto, J., Sampaio, P., & Moura, R. S. (2023, March). Comparative study of e-cigarette aerosol and cigarette smoke effect on ex vivo embryonic chick lung explants. Toxicology Letters. Elsevier BV. http://doi.org/10.1016/j.toxlet.2023.01.002
0378-4274
10.1016/j.toxlet.2023.01.002
36638931
https://www.sciencedirect.com/science/article/pii/S0378427423000085
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132675100901376