Convergent adaptation of multiple herbicide resistance to auxin mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/20.500.12207/6064 |
Resumo: | Herbicide-resistant weeds have been identified and recorded on every continent where croplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found throughout temperate North and South America, and it is a frequent weed among winter cereal crops in Argentina and in Mexico. Broadleaf weed control is based on glyphosate that is used prior to sowing and sulfonylureas or mimic auxin herbicides that are used once the weeds have already emerged. This study was aimed at determining whether a convergent phenotypic adaptation to multiple herbicides had occurred in B. rapa populations from Mexico and Argentina by comparing the herbicide sensitivity to inhibitors of the acetolactate synthase (ALS), 5-enolpyruvylshikimate-3- phosphate (EPSPS), and auxin mimics. Five B. rapa populations were analyzed from seeds collected in wheat fields in Argentina (Ar1 and Ar2) and barley fields in Mexico (Mx1, Mx2 and MxS). Mx1, Mx2, and Ar1 populations presented multiple resistance to ALS- and EPSPS-inhibitors and to auxin mimics (2,4-D, MCPA, and fluroxypyr), while the Ar2 population showed resistance only to ALSinhibitors and glyphosate. Resistance factors ranged from 947 to 4069 for tribenuron-methyl, from 1.5 to 9.4 for 2,4-D, and from 2.7 to 42 for glyphosate. These were consistent with ALS activity, ethylene production, and shikimate accumulation analyses in response to tribenuron-methyl, 2,4-D, and glyphosate, respectively. These results fully support the evolution of the multiple- and crossherbicide resistance to glyphosate, ALS-inhibitors, and auxinic herbicides in B. rapa populations from Mexico and Argentina. |
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Convergent adaptation of multiple herbicide resistance to auxin mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South AmericaALS enzyme activityEthylene productionHerbicide tolerant cropsOilseed rapeResistant gene flowShikimic acid accumulationHerbicide-resistant weeds have been identified and recorded on every continent where croplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found throughout temperate North and South America, and it is a frequent weed among winter cereal crops in Argentina and in Mexico. Broadleaf weed control is based on glyphosate that is used prior to sowing and sulfonylureas or mimic auxin herbicides that are used once the weeds have already emerged. This study was aimed at determining whether a convergent phenotypic adaptation to multiple herbicides had occurred in B. rapa populations from Mexico and Argentina by comparing the herbicide sensitivity to inhibitors of the acetolactate synthase (ALS), 5-enolpyruvylshikimate-3- phosphate (EPSPS), and auxin mimics. Five B. rapa populations were analyzed from seeds collected in wheat fields in Argentina (Ar1 and Ar2) and barley fields in Mexico (Mx1, Mx2 and MxS). Mx1, Mx2, and Ar1 populations presented multiple resistance to ALS- and EPSPS-inhibitors and to auxin mimics (2,4-D, MCPA, and fluroxypyr), while the Ar2 population showed resistance only to ALSinhibitors and glyphosate. Resistance factors ranged from 947 to 4069 for tribenuron-methyl, from 1.5 to 9.4 for 2,4-D, and from 2.7 to 42 for glyphosate. These were consistent with ALS activity, ethylene production, and shikimate accumulation analyses in response to tribenuron-methyl, 2,4-D, and glyphosate, respectively. These results fully support the evolution of the multiple- and crossherbicide resistance to glyphosate, ALS-inhibitors, and auxinic herbicides in B. rapa populations from Mexico and Argentina.MDPI2023-11-30T11:21:35Z2023-05-26T00:00:00Z2023-05-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12207/6064eng2223-7747https://doi.org/10.3390/plants12112119Domínguez-Valenzuela, JoséPalma-Bautista, CandelarioVázquez-Garcia, JoséYanniccari, MarcosGigón, RamónAlcántara-de la Cruz, RicardoDe Prado, RafaelPortugal, Joãoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-07T09:10:10Zoai:repositorio.ipbeja.pt:20.500.12207/6064Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:41:28.612823Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Convergent adaptation of multiple herbicide resistance to auxin mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America |
title |
Convergent adaptation of multiple herbicide resistance to auxin mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America |
spellingShingle |
Convergent adaptation of multiple herbicide resistance to auxin mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America Domínguez-Valenzuela, José ALS enzyme activity Ethylene production Herbicide tolerant crops Oilseed rape Resistant gene flow Shikimic acid accumulation |
title_short |
Convergent adaptation of multiple herbicide resistance to auxin mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America |
title_full |
Convergent adaptation of multiple herbicide resistance to auxin mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America |
title_fullStr |
Convergent adaptation of multiple herbicide resistance to auxin mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America |
title_full_unstemmed |
Convergent adaptation of multiple herbicide resistance to auxin mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America |
title_sort |
Convergent adaptation of multiple herbicide resistance to auxin mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America |
author |
Domínguez-Valenzuela, José |
author_facet |
Domínguez-Valenzuela, José Palma-Bautista, Candelario Vázquez-Garcia, José Yanniccari, Marcos Gigón, Ramón Alcántara-de la Cruz, Ricardo De Prado, Rafael Portugal, João |
author_role |
author |
author2 |
Palma-Bautista, Candelario Vázquez-Garcia, José Yanniccari, Marcos Gigón, Ramón Alcántara-de la Cruz, Ricardo De Prado, Rafael Portugal, João |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Domínguez-Valenzuela, José Palma-Bautista, Candelario Vázquez-Garcia, José Yanniccari, Marcos Gigón, Ramón Alcántara-de la Cruz, Ricardo De Prado, Rafael Portugal, João |
dc.subject.por.fl_str_mv |
ALS enzyme activity Ethylene production Herbicide tolerant crops Oilseed rape Resistant gene flow Shikimic acid accumulation |
topic |
ALS enzyme activity Ethylene production Herbicide tolerant crops Oilseed rape Resistant gene flow Shikimic acid accumulation |
description |
Herbicide-resistant weeds have been identified and recorded on every continent where croplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found throughout temperate North and South America, and it is a frequent weed among winter cereal crops in Argentina and in Mexico. Broadleaf weed control is based on glyphosate that is used prior to sowing and sulfonylureas or mimic auxin herbicides that are used once the weeds have already emerged. This study was aimed at determining whether a convergent phenotypic adaptation to multiple herbicides had occurred in B. rapa populations from Mexico and Argentina by comparing the herbicide sensitivity to inhibitors of the acetolactate synthase (ALS), 5-enolpyruvylshikimate-3- phosphate (EPSPS), and auxin mimics. Five B. rapa populations were analyzed from seeds collected in wheat fields in Argentina (Ar1 and Ar2) and barley fields in Mexico (Mx1, Mx2 and MxS). Mx1, Mx2, and Ar1 populations presented multiple resistance to ALS- and EPSPS-inhibitors and to auxin mimics (2,4-D, MCPA, and fluroxypyr), while the Ar2 population showed resistance only to ALSinhibitors and glyphosate. Resistance factors ranged from 947 to 4069 for tribenuron-methyl, from 1.5 to 9.4 for 2,4-D, and from 2.7 to 42 for glyphosate. These were consistent with ALS activity, ethylene production, and shikimate accumulation analyses in response to tribenuron-methyl, 2,4-D, and glyphosate, respectively. These results fully support the evolution of the multiple- and crossherbicide resistance to glyphosate, ALS-inhibitors, and auxinic herbicides in B. rapa populations from Mexico and Argentina. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-11-30T11:21:35Z 2023-05-26T00:00:00Z 2023-05-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/20.500.12207/6064 |
url |
https://hdl.handle.net/20.500.12207/6064 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2223-7747 https://doi.org/10.3390/plants12112119 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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