Seminal plasma modulates miRNA expression by sow genital tract lining explants

Detalhes bibliográficos
Autor(a) principal: Barranco, Isabel
Data de Publicação: 2020
Outros Autores: Padilla, Lorena, Martinez, Cristina A., Alvarez-Rodriguez, Manuel, Parrilla, Inmaculada, Lucas, Xiomara, Ferreira-Dias, Graça, Yeste, Marc, Rodriguez-Martinez, Heriberto, Roca, Jordi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/24101
Resumo: Research Areas: Biochemistry & Molecular Biology
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spelling Seminal plasma modulates miRNA expression by sow genital tract lining explantsExplantsFemale genital tractImmune responsemiRNAsMucosal tissueSeminal plasmaTranscriptomeResearch Areas: Biochemistry & Molecular BiologyThe seminal plasma (SP) modulates the female reproductive immune environment after mating, and microRNAs (miRNAs) could participate in the process. Considering that the boar ejaculate is built by fractions di ering in SP-composition, this study evaluated whether exposure of mucosal explants of the sow internal genital tract (uterus, utero-tubal junction and isthmus) to di erent SP-fractions changed the profile of explant-secreted miRNAs. Mucosal explants retrieved from oestrus sows (n = 3) were in vitro exposed to: Medium 199 (M199, Control) or M199 supplemented (1:40 v/v) with SP from the sperm-rich fraction (SRF), the post-SRF or the entire recomposed ejaculate, for 16 h. After, the explants were cultured in M199 for 24 h to finally collect the media for miRNA analyses using GeneChip miRNA 4.0 Array (A ymetrix). Fifteen di erentially expressed (False Discovery Rate (FDR) < 0.05 and Fold-change 2) miRNAs (11 down- versus 4 up-regulated) were identified (the most in the media of uterine explants incubated with SP from post-SRF). Bioinformatics analysis identified that predicted target genes of dysregulated miRNAs, mainly miR-34b, miR-205, miR-4776-3p and miR-574-5p, were involved in functions and pathways related to immune response. In conclusion, SP is able to elicit changes in the miRNAs profile secreted by female genital tract, ultimately depending SP-composition.MDPIRepositório da Universidade de LisboaBarranco, IsabelPadilla, LorenaMartinez, Cristina A.Alvarez-Rodriguez, ManuelParrilla, InmaculadaLucas, XiomaraFerreira-Dias, GraçaYeste, MarcRodriguez-Martinez, HeribertoRoca, Jordi2022-04-18T16:03:05Z2020-06-192020-06-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24101engBarranco I, Padilla L, Martinez CA, Alvarez-Rodriguez M, Parrilla I, Lucas X, Ferreira-Dias G, Yeste M, Rodriguez-Martinez H, Roca J. 2020. Seminal plasma modulates miRNA expression by sow genital tract lining explants, Biomolecules 10(6):933. Doi 10.3390/biom100609332218-273X10.3390/biom10060933info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:44Zoai:www.repository.utl.pt:10400.5/24101Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:08:13.365112Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Seminal plasma modulates miRNA expression by sow genital tract lining explants
title Seminal plasma modulates miRNA expression by sow genital tract lining explants
spellingShingle Seminal plasma modulates miRNA expression by sow genital tract lining explants
Barranco, Isabel
Explants
Female genital tract
Immune response
miRNAs
Mucosal tissue
Seminal plasma
Transcriptome
title_short Seminal plasma modulates miRNA expression by sow genital tract lining explants
title_full Seminal plasma modulates miRNA expression by sow genital tract lining explants
title_fullStr Seminal plasma modulates miRNA expression by sow genital tract lining explants
title_full_unstemmed Seminal plasma modulates miRNA expression by sow genital tract lining explants
title_sort Seminal plasma modulates miRNA expression by sow genital tract lining explants
author Barranco, Isabel
author_facet Barranco, Isabel
Padilla, Lorena
Martinez, Cristina A.
Alvarez-Rodriguez, Manuel
Parrilla, Inmaculada
Lucas, Xiomara
Ferreira-Dias, Graça
Yeste, Marc
Rodriguez-Martinez, Heriberto
Roca, Jordi
author_role author
author2 Padilla, Lorena
Martinez, Cristina A.
Alvarez-Rodriguez, Manuel
Parrilla, Inmaculada
Lucas, Xiomara
Ferreira-Dias, Graça
Yeste, Marc
Rodriguez-Martinez, Heriberto
Roca, Jordi
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Barranco, Isabel
Padilla, Lorena
Martinez, Cristina A.
Alvarez-Rodriguez, Manuel
Parrilla, Inmaculada
Lucas, Xiomara
Ferreira-Dias, Graça
Yeste, Marc
Rodriguez-Martinez, Heriberto
Roca, Jordi
dc.subject.por.fl_str_mv Explants
Female genital tract
Immune response
miRNAs
Mucosal tissue
Seminal plasma
Transcriptome
topic Explants
Female genital tract
Immune response
miRNAs
Mucosal tissue
Seminal plasma
Transcriptome
description Research Areas: Biochemistry & Molecular Biology
publishDate 2020
dc.date.none.fl_str_mv 2020-06-19
2020-06-19T00:00:00Z
2022-04-18T16:03:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/24101
url http://hdl.handle.net/10400.5/24101
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Barranco I, Padilla L, Martinez CA, Alvarez-Rodriguez M, Parrilla I, Lucas X, Ferreira-Dias G, Yeste M, Rodriguez-Martinez H, Roca J. 2020. Seminal plasma modulates miRNA expression by sow genital tract lining explants, Biomolecules 10(6):933. Doi 10.3390/biom10060933
2218-273X
10.3390/biom10060933
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131176094400512

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