Tailored pretreatment of serum samples and biomarker extraction afforded by ionic liquids as constituents of aqueous biphasic systems
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/38062 |
Resumo: | Prostate cancer serum biomarkers, such as the U.S. Food and Drug Administration (FDA)-approved prostate specific antigen (PSA), are valuable markers of diagnosis, response to treatment and disease progression. Despite the expected benefits of using PSA to better manage the mortality and morbidity associated to prostate cancer, sample complexity and interference within analysis make its quantification in serum a high-cost and strenuous assignment. Here, we make use of the simple preparation, water-rich environment, and selectivity of aqueous biphasic systems (ABS) to develop alternative serum pretreatment strategies. A set of ammonium- and phosphonium-based ionic liquids (ILs) were used as either main phase-forming agents in IL-salt ABS or as adjuvants in polymer-salt ABS, allowing to explore the IL designer solvent nature and versatile role in ABS formation and performance. Serum pretreatment efficiency was evaluated by the capacity of each ABS to simultaneously deplete high abundance serum proteins (immunoglobulin G, IgG, and human serum albumin, HSA) at the systems’ interphase (thus, generating a three-phase partitioning system – ABS-TPP) and extract PSA to a single ABS phase. Regardless of the ABS typology under appraisal (IL-salt or polymer-salt-IL), the proper design of the IL structure is critical for both the ability to deplete high abundance serum proteins and extract PSA. The results demonstrate that, in addition to the proper choice of the ABS typology, a careful adjustment of the IL hydrophobicity is key to achieve the simultaneous depletion of high-abundance proteins and complete PSA extraction in a single step, creating new approaches for PSA analysis and its application in diagnosis/prognosis. |
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Tailored pretreatment of serum samples and biomarker extraction afforded by ionic liquids as constituents of aqueous biphasic systemsProstate specific antigenHuman serum pretreatmentBiomarker extractionAqueous biphasic systemPolymerIonic liquidProstate cancer serum biomarkers, such as the U.S. Food and Drug Administration (FDA)-approved prostate specific antigen (PSA), are valuable markers of diagnosis, response to treatment and disease progression. Despite the expected benefits of using PSA to better manage the mortality and morbidity associated to prostate cancer, sample complexity and interference within analysis make its quantification in serum a high-cost and strenuous assignment. Here, we make use of the simple preparation, water-rich environment, and selectivity of aqueous biphasic systems (ABS) to develop alternative serum pretreatment strategies. A set of ammonium- and phosphonium-based ionic liquids (ILs) were used as either main phase-forming agents in IL-salt ABS or as adjuvants in polymer-salt ABS, allowing to explore the IL designer solvent nature and versatile role in ABS formation and performance. Serum pretreatment efficiency was evaluated by the capacity of each ABS to simultaneously deplete high abundance serum proteins (immunoglobulin G, IgG, and human serum albumin, HSA) at the systems’ interphase (thus, generating a three-phase partitioning system – ABS-TPP) and extract PSA to a single ABS phase. Regardless of the ABS typology under appraisal (IL-salt or polymer-salt-IL), the proper design of the IL structure is critical for both the ability to deplete high abundance serum proteins and extract PSA. The results demonstrate that, in addition to the proper choice of the ABS typology, a careful adjustment of the IL hydrophobicity is key to achieve the simultaneous depletion of high-abundance proteins and complete PSA extraction in a single step, creating new approaches for PSA analysis and its application in diagnosis/prognosis.Elsevier2025-06-03T00:00:00Z2023-06-03T00:00:00Z2023-06-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/38062eng1383-586610.1016/j.seppur.2023.124248Rosa, Marguerita E.Mendes, Maria S. M.Carmo, EduardaConde, João P.Coutinho, João A. P.Freire, Mara G.Silva, Francisca A.info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:14:25Zoai:ria.ua.pt:10773/38062Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:08:39.204886Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Tailored pretreatment of serum samples and biomarker extraction afforded by ionic liquids as constituents of aqueous biphasic systems |
title |
Tailored pretreatment of serum samples and biomarker extraction afforded by ionic liquids as constituents of aqueous biphasic systems |
spellingShingle |
Tailored pretreatment of serum samples and biomarker extraction afforded by ionic liquids as constituents of aqueous biphasic systems Rosa, Marguerita E. Prostate specific antigen Human serum pretreatment Biomarker extraction Aqueous biphasic system Polymer Ionic liquid |
title_short |
Tailored pretreatment of serum samples and biomarker extraction afforded by ionic liquids as constituents of aqueous biphasic systems |
title_full |
Tailored pretreatment of serum samples and biomarker extraction afforded by ionic liquids as constituents of aqueous biphasic systems |
title_fullStr |
Tailored pretreatment of serum samples and biomarker extraction afforded by ionic liquids as constituents of aqueous biphasic systems |
title_full_unstemmed |
Tailored pretreatment of serum samples and biomarker extraction afforded by ionic liquids as constituents of aqueous biphasic systems |
title_sort |
Tailored pretreatment of serum samples and biomarker extraction afforded by ionic liquids as constituents of aqueous biphasic systems |
author |
Rosa, Marguerita E. |
author_facet |
Rosa, Marguerita E. Mendes, Maria S. M. Carmo, Eduarda Conde, João P. Coutinho, João A. P. Freire, Mara G. Silva, Francisca A. |
author_role |
author |
author2 |
Mendes, Maria S. M. Carmo, Eduarda Conde, João P. Coutinho, João A. P. Freire, Mara G. Silva, Francisca A. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Rosa, Marguerita E. Mendes, Maria S. M. Carmo, Eduarda Conde, João P. Coutinho, João A. P. Freire, Mara G. Silva, Francisca A. |
dc.subject.por.fl_str_mv |
Prostate specific antigen Human serum pretreatment Biomarker extraction Aqueous biphasic system Polymer Ionic liquid |
topic |
Prostate specific antigen Human serum pretreatment Biomarker extraction Aqueous biphasic system Polymer Ionic liquid |
description |
Prostate cancer serum biomarkers, such as the U.S. Food and Drug Administration (FDA)-approved prostate specific antigen (PSA), are valuable markers of diagnosis, response to treatment and disease progression. Despite the expected benefits of using PSA to better manage the mortality and morbidity associated to prostate cancer, sample complexity and interference within analysis make its quantification in serum a high-cost and strenuous assignment. Here, we make use of the simple preparation, water-rich environment, and selectivity of aqueous biphasic systems (ABS) to develop alternative serum pretreatment strategies. A set of ammonium- and phosphonium-based ionic liquids (ILs) were used as either main phase-forming agents in IL-salt ABS or as adjuvants in polymer-salt ABS, allowing to explore the IL designer solvent nature and versatile role in ABS formation and performance. Serum pretreatment efficiency was evaluated by the capacity of each ABS to simultaneously deplete high abundance serum proteins (immunoglobulin G, IgG, and human serum albumin, HSA) at the systems’ interphase (thus, generating a three-phase partitioning system – ABS-TPP) and extract PSA to a single ABS phase. Regardless of the ABS typology under appraisal (IL-salt or polymer-salt-IL), the proper design of the IL structure is critical for both the ability to deplete high abundance serum proteins and extract PSA. The results demonstrate that, in addition to the proper choice of the ABS typology, a careful adjustment of the IL hydrophobicity is key to achieve the simultaneous depletion of high-abundance proteins and complete PSA extraction in a single step, creating new approaches for PSA analysis and its application in diagnosis/prognosis. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-06-03T00:00:00Z 2023-06-03 2025-06-03T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/38062 |
url |
http://hdl.handle.net/10773/38062 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1383-5866 10.1016/j.seppur.2023.124248 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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