Plasma versus Serum Analysis by FTIR Spectroscopy to Capture the Human Physiological State

Detalhes bibliográficos
Autor(a) principal: Araújo, Rúben
Data de Publicação: 2022
Outros Autores: Ramalhete, Luís, Ribeiro, Edna, Calado, Cecília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/147240
Resumo: Funding Information: This work was supported by the project grant DSAIPA/DS/0117/2020 supported by Fundação para a Ciência e a Tecnologia, Portugal; and by the project grant NeproMD/ISEL/2020 financed by Instituto Politécnico de Lisboa (IPL). The present work was conducted in H&TRC- Health & Technology Research Center, Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa and in the Engineering and Health Laboratory, that resulted from a collaboration protocol established between Universidade Católica Portuguesa and Instituto Politécnico de Lisboa. Publisher Copyright: © 2022 by the authors.
id RCAP_861d446b6d9b2b38cdbbd1b37613e10b
oai_identifier_str oai:run.unl.pt:10362/147240
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Plasma versus Serum Analysis by FTIR Spectroscopy to Capture the Human Physiological Stateepigallocatechin-3-gallateFTIR spectroscopyplasmaserumBiotechnologyBioengineeringBiochemistryBiomedical EngineeringApplied Microbiology and BiotechnologyFunding Information: This work was supported by the project grant DSAIPA/DS/0117/2020 supported by Fundação para a Ciência e a Tecnologia, Portugal; and by the project grant NeproMD/ISEL/2020 financed by Instituto Politécnico de Lisboa (IPL). The present work was conducted in H&TRC- Health & Technology Research Center, Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa and in the Engineering and Health Laboratory, that resulted from a collaboration protocol established between Universidade Católica Portuguesa and Instituto Politécnico de Lisboa. Publisher Copyright: © 2022 by the authors.Fourier Transform InfraRed spectroscopy of serum and plasma has been highly explored for medical diagnosis, due to its general simplicity, and high sensitivity and specificity. To evaluate the plasma and serum molecular fingerprint, as obtained by FTIR spectroscopy, to acquire the system metabolic state, serum and plasma spectra were compared to characterize the metabolic state of 30 human volunteers, between 90 days consumption of green tea extract rich in Epigallocatechin-3-gallate (EGCG). Both plasma and serum spectra enabled the high impact of EGCG consumption on the biofluid spectra to be observed, as analyzed by the spectra principal component analysis, hierarchical-cluster analysis, and univariate data analysis. Plasma spectra resulted in the prediction of EGCG consumption with a slightly higher specificity, accuracy, and precision, also pointing to a higher number of significant spectral bands that were different between the 90 days period. Despite this, the lipid regions of the serum spectra were more affected by EGCG consumption than the corresponding plasma spectra. Therefore, in general, if no specific compound analysis is highlighted, plasma is in general the advised biofluid to capture by FTIR spectroscopy the general metabolic state. If the lipid content of the biofluid is relevant, serum spectra could present some advantages over plasma spectra.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Comprehensive Health Research Centre (CHRC) - pólo NMSRUNAraújo, RúbenRamalhete, LuísRibeiro, EdnaCalado, Cecília2023-01-09T22:16:43Z2022-122022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/147240eng2673-6284PURE: 49559403https://doi.org/10.3390/biotech11040056info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:07:54Zoai:run.unl.pt:10362/147240Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:07:54Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Plasma versus Serum Analysis by FTIR Spectroscopy to Capture the Human Physiological State
title Plasma versus Serum Analysis by FTIR Spectroscopy to Capture the Human Physiological State
spellingShingle Plasma versus Serum Analysis by FTIR Spectroscopy to Capture the Human Physiological State
Araújo, Rúben
epigallocatechin-3-gallate
FTIR spectroscopy
plasma
serum
Biotechnology
Bioengineering
Biochemistry
Biomedical Engineering
Applied Microbiology and Biotechnology
title_short Plasma versus Serum Analysis by FTIR Spectroscopy to Capture the Human Physiological State
title_full Plasma versus Serum Analysis by FTIR Spectroscopy to Capture the Human Physiological State
title_fullStr Plasma versus Serum Analysis by FTIR Spectroscopy to Capture the Human Physiological State
title_full_unstemmed Plasma versus Serum Analysis by FTIR Spectroscopy to Capture the Human Physiological State
title_sort Plasma versus Serum Analysis by FTIR Spectroscopy to Capture the Human Physiological State
author Araújo, Rúben
author_facet Araújo, Rúben
Ramalhete, Luís
Ribeiro, Edna
Calado, Cecília
author_role author
author2 Ramalhete, Luís
Ribeiro, Edna
Calado, Cecília
author2_role author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Comprehensive Health Research Centre (CHRC) - pólo NMS
RUN
dc.contributor.author.fl_str_mv Araújo, Rúben
Ramalhete, Luís
Ribeiro, Edna
Calado, Cecília
dc.subject.por.fl_str_mv epigallocatechin-3-gallate
FTIR spectroscopy
plasma
serum
Biotechnology
Bioengineering
Biochemistry
Biomedical Engineering
Applied Microbiology and Biotechnology
topic epigallocatechin-3-gallate
FTIR spectroscopy
plasma
serum
Biotechnology
Bioengineering
Biochemistry
Biomedical Engineering
Applied Microbiology and Biotechnology
description Funding Information: This work was supported by the project grant DSAIPA/DS/0117/2020 supported by Fundação para a Ciência e a Tecnologia, Portugal; and by the project grant NeproMD/ISEL/2020 financed by Instituto Politécnico de Lisboa (IPL). The present work was conducted in H&TRC- Health & Technology Research Center, Escola Superior de Tecnologia da Saúde de Lisboa, Instituto Politécnico de Lisboa and in the Engineering and Health Laboratory, that resulted from a collaboration protocol established between Universidade Católica Portuguesa and Instituto Politécnico de Lisboa. Publisher Copyright: © 2022 by the authors.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
2022-12-01T00:00:00Z
2023-01-09T22:16:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/147240
url http://hdl.handle.net/10362/147240
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2673-6284
PURE: 49559403
https://doi.org/10.3390/biotech11040056
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817545907123519488

Registros relacionados