Insights on catheter-related bloodstream infections: a prospective observational study on the catheter colonization and multi-drug resistance

Detalhes bibliográficos
Autor(a) principal: Pinto, Miguel
Data de Publicação: 2022
Outros Autores: Borges, Vítor, Nascimento, Maria, Martins, Filomena, Pessanha, Maria Ana, Faria, Isabel, Rodrigues, João, Matias, Rui, Gomes, João Paulo, Jordao, Luisa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8321
Resumo: Observational Study
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spelling Insights on catheter-related bloodstream infections: a prospective observational study on the catheter colonization and multi-drug resistanceCatheter-related Bloodstream InfectionCatheter-Related InfectionsStaphylococcus SpidermidisStaphylococcus aureusBiofilmsAntimicrobial ResistanceWhole-genome SequencingResistência aos AntimicrobianosObservational StudyBackground: Central venous catheter-related bloodstream infection (CRBSI) is a huge public health concern with considerable impact on mortality and health costs. Aim: A three-year observational study enrolling three tertiary hospitals located in Lisbon, Portugal, was designed to identify the major aetiological agents of CRBSI, their ability to colonize central venous catheters and their antimicrobial resistance profiles. Methods: Aetiological agents of CRBSI were identified by Vitek 2. Whole-genome sequencing was used to confirm CRBSI by the most prevalent aetiological agents and characterize their resistome. Central venous catheter colonization (namely by biofilm assembly) was monitored by scanning electron microscopy. Findings: Staphylococci were the most prevalent causative agent (36/58, 62.0%), with S. aureus and coagulase-negative S. epidermidis accounting for 24.1% and 36.2% of CRBSIs, respectively. Fifty-nine of 72 staphylococci isolates were meticillin resistant. Comparative genomic analysis of central venous catheters/haemoculture pairs of isolates revealed genomic matches for 35 of 36 pairs and a good correlation between antibiotic susceptibility phenotype and the presence of antimicrobial resistance genetic determinants. Biofilms were present on 48.6% of the central venous catheters; nevertheless, no statistically significant association was established between biofilm assembly and CRBSI, and the presence/absence of ica operon and agr groups did not correlate with biofilm phenotypes, highlighting the need for further studies to elucidate biofilms' role on this healthcare-associated infection. Conclusion: Whole-genome sequencing was shown to be a valuable tool to confirm CRBSI. Although more than 42.3% of the central venous catheters were colonized by staphylococci, no statistically significant association was found between CRBSI and biofilms.This research was partially funded by Fundação para a Ciência e a Tecnologia (FCT) as part of the Bilateral Cooperation Program between Portugal and Slovakia 2019-2021 (Grant FCT/487/15/01/2019/S).Elsevier/The Healthcare Infection SocietyRepositório Científico do Instituto Nacional de SaúdePinto, MiguelBorges, VítorNascimento, MariaMartins, FilomenaPessanha, Maria AnaFaria, IsabelRodrigues, JoãoMatias, RuiGomes, João PauloJordao, Luisa2024-01-01T01:30:56Z2022-052022-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8321engJ Hosp Infect. 2022 May;123:43-51. doi: 10.1016/j.jhin.2022.01.025. Epub 2022 Feb 180195-670110.1016/j.jhin.2022.01.025info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-06T01:31:51Zoai:repositorio.insa.pt:10400.18/8321Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:45.323979Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Insights on catheter-related bloodstream infections: a prospective observational study on the catheter colonization and multi-drug resistance
title Insights on catheter-related bloodstream infections: a prospective observational study on the catheter colonization and multi-drug resistance
spellingShingle Insights on catheter-related bloodstream infections: a prospective observational study on the catheter colonization and multi-drug resistance
Pinto, Miguel
Catheter-related Bloodstream Infection
Catheter-Related Infections
Staphylococcus Spidermidis
Staphylococcus aureus
Biofilms
Antimicrobial Resistance
Whole-genome Sequencing
Resistência aos Antimicrobianos
title_short Insights on catheter-related bloodstream infections: a prospective observational study on the catheter colonization and multi-drug resistance
title_full Insights on catheter-related bloodstream infections: a prospective observational study on the catheter colonization and multi-drug resistance
title_fullStr Insights on catheter-related bloodstream infections: a prospective observational study on the catheter colonization and multi-drug resistance
title_full_unstemmed Insights on catheter-related bloodstream infections: a prospective observational study on the catheter colonization and multi-drug resistance
title_sort Insights on catheter-related bloodstream infections: a prospective observational study on the catheter colonization and multi-drug resistance
author Pinto, Miguel
author_facet Pinto, Miguel
Borges, Vítor
Nascimento, Maria
Martins, Filomena
Pessanha, Maria Ana
Faria, Isabel
Rodrigues, João
Matias, Rui
Gomes, João Paulo
Jordao, Luisa
author_role author
author2 Borges, Vítor
Nascimento, Maria
Martins, Filomena
Pessanha, Maria Ana
Faria, Isabel
Rodrigues, João
Matias, Rui
Gomes, João Paulo
Jordao, Luisa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Pinto, Miguel
Borges, Vítor
Nascimento, Maria
Martins, Filomena
Pessanha, Maria Ana
Faria, Isabel
Rodrigues, João
Matias, Rui
Gomes, João Paulo
Jordao, Luisa
dc.subject.por.fl_str_mv Catheter-related Bloodstream Infection
Catheter-Related Infections
Staphylococcus Spidermidis
Staphylococcus aureus
Biofilms
Antimicrobial Resistance
Whole-genome Sequencing
Resistência aos Antimicrobianos
topic Catheter-related Bloodstream Infection
Catheter-Related Infections
Staphylococcus Spidermidis
Staphylococcus aureus
Biofilms
Antimicrobial Resistance
Whole-genome Sequencing
Resistência aos Antimicrobianos
description Observational Study
publishDate 2022
dc.date.none.fl_str_mv 2022-05
2022-05-01T00:00:00Z
2024-01-01T01:30:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8321
url http://hdl.handle.net/10400.18/8321
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv J Hosp Infect. 2022 May;123:43-51. doi: 10.1016/j.jhin.2022.01.025. Epub 2022 Feb 18
0195-6701
10.1016/j.jhin.2022.01.025
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier/The Healthcare Infection Society
publisher.none.fl_str_mv Elsevier/The Healthcare Infection Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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