Timolol modulates erythrocyte nitric oxide bioavailability

Detalhes bibliográficos
Autor(a) principal: Saldanha, Carlota
Data de Publicação: 2013
Outros Autores: Teixeira, Pedro, Santos-Freitas, T., Napoleão, Patricia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/10675
Resumo: © 2013 Saldanha C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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spelling Timolol modulates erythrocyte nitric oxide bioavailabilityErythrocyteNitric oxideTimolol maleateAcetylcholineS-nitrosoglutathioneSodium chloride (NaCl)© 2013 Saldanha C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Changes of oxygen partial pressure in tissues are sensed by the erythrocytes that with the efflux or with the maintenance of nitric oxide that contains promote vasodilation or vasoconstriction. Binding of acetylcholine with the acetylcholinesterase of the erythrocyte membrane originates a signal transduction mechanism that involves both the Gi protein as well as band 3 protein what stimulates nitric oxide efflux. The bioavailability of nitric oxide in presence of velnacrine maleate an inhibitor of acetylcholinesterase is preserved what means that there are no changes in the nitric oxide efflux. Timolol maleate is an inhibitor of acetylcholinesterase. The aim of this study was to assess the role of the timolol maleate in the erythrocyte in respect to bioavailability on nitric oxide and compare it with effect resulting of the presence of acetylcholine. Venous blood samples were collected from the forearm vein of fifteen healthy Caucasian men after informed consent. Each blood sample was divided in three 1 mL samples, centrifuged, and suspensions of erythrocyte were performed in order to achieve 10 μM final concentration either of acetylcholine or of the timolol. Levels of nitric oxide efflux were evaluated by amperometric method. S-nitrosoglutathione, nitrites and nitrates were assessed with the Griess reaction using the spectrophotometric method. The nitric oxide efflux by the erythrocyte in presence of timolol is like to the control sample but significantly decreased it when compared to the sample with acetylcholine. The presence of timolol induces decrease in the erythrocyte levels of S-nitrosoglutathione significantly in relation with the control and with acetylcholine samples. In conclusion, in vitro, in erythrocyte the nitric oxide content is maintained by timolol maleate. It may be expected the same role for timolol in the ocular microcirculation when being applied as a therapeutic compound.This study was supported by grants from the FCT - Fundação para a Ciência e a TecnologiaOMICS Publishing GroupRepositório da Universidade de LisboaSaldanha, CarlotaTeixeira, PedroSantos-Freitas, T.Napoleão, Patricia2014-03-04T17:08:20Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10675engJ Clin Exp Ophthalmol 2013, 4:32155-9570http://dx.doi.org/10.4172/2155-9570.1000285metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:56:19Zoai:repositorio.ul.pt:10451/10675Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:34:37.121951Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Timolol modulates erythrocyte nitric oxide bioavailability
title Timolol modulates erythrocyte nitric oxide bioavailability
spellingShingle Timolol modulates erythrocyte nitric oxide bioavailability
Saldanha, Carlota
Erythrocyte
Nitric oxide
Timolol maleate
Acetylcholine
S-nitrosoglutathione
Sodium chloride (NaCl)
title_short Timolol modulates erythrocyte nitric oxide bioavailability
title_full Timolol modulates erythrocyte nitric oxide bioavailability
title_fullStr Timolol modulates erythrocyte nitric oxide bioavailability
title_full_unstemmed Timolol modulates erythrocyte nitric oxide bioavailability
title_sort Timolol modulates erythrocyte nitric oxide bioavailability
author Saldanha, Carlota
author_facet Saldanha, Carlota
Teixeira, Pedro
Santos-Freitas, T.
Napoleão, Patricia
author_role author
author2 Teixeira, Pedro
Santos-Freitas, T.
Napoleão, Patricia
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Saldanha, Carlota
Teixeira, Pedro
Santos-Freitas, T.
Napoleão, Patricia
dc.subject.por.fl_str_mv Erythrocyte
Nitric oxide
Timolol maleate
Acetylcholine
S-nitrosoglutathione
Sodium chloride (NaCl)
topic Erythrocyte
Nitric oxide
Timolol maleate
Acetylcholine
S-nitrosoglutathione
Sodium chloride (NaCl)
description © 2013 Saldanha C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
2014-03-04T17:08:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10675
url http://hdl.handle.net/10451/10675
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv J Clin Exp Ophthalmol 2013, 4:3
2155-9570
http://dx.doi.org/10.4172/2155-9570.1000285
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv OMICS Publishing Group
publisher.none.fl_str_mv OMICS Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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