Drivers of genetic diversity in secondary metabolic gene clusters within a fungal species
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/48385 |
Resumo: | Drivers of genetic diversity in secondary metabolic gene clusters within a fungal species |
| id |
RCAP_87eecfde0c52f876c2c99ae713975ad1 |
|---|---|
| oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/48385 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
Drivers of genetic diversity in secondary metabolic gene clusters within a fungal speciesScience & TechnologyDrivers of genetic diversity in secondary metabolic gene clusters within a fungal speciesFilamentous fungi produce a diverse array of secondary metabolites (SMs) critical for defense, virulence, and communication. The metabolic pathways that produce SMs are found in contiguous gene clusters in fungal genomes, an atypical arrangement for metabolic pathways in other eukaryotes. Comparative studies of filamentous fungal species have shown that SM gene clusters are often either highly divergent or uniquely present in one or a handful of species, hampering efforts to determine the genetic basis and evolutionary drivers of SM gene cluster divergence. Here, we examined SM variation in 66 cosmopolitan strains of a single species, the opportunistic human pathogen Aspergillus fumigatus. Investigation of genome-wide within-species variation revealed 5 general types of variation in SM gene clusters: nonfunctional gene polymorphisms; gene gain and loss polymorphisms; whole cluster gain and loss polymorphisms; allelic polymorphisms, in which different alleles corresponded to distinct, nonhomologous clusters; and location polymorphisms, in which a cluster was found to differ in its genomic location across strains. These polymorphisms affect the function of representative A. fumigatus SM gene clusters, such as those involved in the production of gliotoxin, fumigaclavine, and helvolic acid as well as the function of clusters with undefined products. In addition to enabling the identification of polymorphisms, the detection of which requires extensive genome-wide synteny conservation (e.g., mobile gene clusters and nonhomologous cluster alleles), our approach also implicated multiple underlying genetic drivers, including point mutations, recombination, and genomic deletion and insertion events as well as horizontal gene transfer from distant fungi. Finally, most of the variants that we uncover within A. fumigatus have been previously hypothesized to contribute to SM gene cluster diversity across entire fungal classes and phyla. We suggest that the drivers of genetic diversity operating within a fungal species shown here are sufficient to explain SM cluster macroevolutionary patterns.National Science Foundation (grant number DEB-1442113). Received by AR. U.S. National Library of Medicine training grant (grant number 2T15LM007450). Received by ALL. Conselho Nacional de Desenvolvimento Cientı´fico e 573 Tecnológico. Northern Portugal Regional Operational Programme (grant number NORTE-01- 0145-FEDER-000013). Received by FR. Fundação de Amparo à Pesquisa do 572 Estado de São Paulo. Received by GHG. National Institutes of Health (grant number R01 AI065728-01). Received by NPK. National Science Foundation (grant number IOS-1401682). Received by JHW. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.info:eu-repo/semantics/publishedVersionPublic Library of Science (PLOS)Universidade do MinhoLind, Abigail L.Wisecaver, Jennifer H.Lameiras, CatarinaWiemann, PhilippPalmer, Jonathan M.Keller, Nancy P.Rodrigues, Fernando José dos SantosGoldman, Gustavo H.Rokas, Antonis2017-11-172017-11-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/48385eng1544-91731545-788510.1371/journal.pbio.200358329149178http://journals.plos.org/plosbiology/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:38:53Zoai:repositorium.sdum.uminho.pt:1822/48385Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:35:25.018755Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Drivers of genetic diversity in secondary metabolic gene clusters within a fungal species |
| title |
Drivers of genetic diversity in secondary metabolic gene clusters within a fungal species |
| spellingShingle |
Drivers of genetic diversity in secondary metabolic gene clusters within a fungal species Lind, Abigail L. Science & Technology |
| title_short |
Drivers of genetic diversity in secondary metabolic gene clusters within a fungal species |
| title_full |
Drivers of genetic diversity in secondary metabolic gene clusters within a fungal species |
| title_fullStr |
Drivers of genetic diversity in secondary metabolic gene clusters within a fungal species |
| title_full_unstemmed |
Drivers of genetic diversity in secondary metabolic gene clusters within a fungal species |
| title_sort |
Drivers of genetic diversity in secondary metabolic gene clusters within a fungal species |
| author |
Lind, Abigail L. |
| author_facet |
Lind, Abigail L. Wisecaver, Jennifer H. Lameiras, Catarina Wiemann, Philipp Palmer, Jonathan M. Keller, Nancy P. Rodrigues, Fernando José dos Santos Goldman, Gustavo H. Rokas, Antonis |
| author_role |
author |
| author2 |
Wisecaver, Jennifer H. Lameiras, Catarina Wiemann, Philipp Palmer, Jonathan M. Keller, Nancy P. Rodrigues, Fernando José dos Santos Goldman, Gustavo H. Rokas, Antonis |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Lind, Abigail L. Wisecaver, Jennifer H. Lameiras, Catarina Wiemann, Philipp Palmer, Jonathan M. Keller, Nancy P. Rodrigues, Fernando José dos Santos Goldman, Gustavo H. Rokas, Antonis |
| dc.subject.por.fl_str_mv |
Science & Technology |
| topic |
Science & Technology |
| description |
Drivers of genetic diversity in secondary metabolic gene clusters within a fungal species |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-11-17 2017-11-17T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/48385 |
| url |
http://hdl.handle.net/1822/48385 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1544-9173 1545-7885 10.1371/journal.pbio.2003583 29149178 http://journals.plos.org/plosbiology/ |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Public Library of Science (PLOS) |
| publisher.none.fl_str_mv |
Public Library of Science (PLOS) |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799132879437955072 |