Study and preparation of multifunctional poly(L-lysine)@hyaluronic acid nanopolyplexes for the effective delivery of tumor suppressive mir-34a into triple-negative breast cancer cells

Detalhes bibliográficos
Autor(a) principal: Djafari, Jamila
Data de Publicação: 2020
Outros Autores: Fernández-Lodeiro, Javier, Santos, Hugo M., Lorenzo, Julia, Rodriguez-Calado, Sergi, Bértolo, Emilia, Capelo-Martínez, José Luis, Lodeiro, Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/117271
Resumo: POCI-01-0145-FEDER-007265 UIDB/04077/2020 PTDC/QEQ-MED/2118/2014 2015 (IF/00007/2015) RTI2018-098027-B-C22
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spelling Study and preparation of multifunctional poly(L-lysine)@hyaluronic acid nanopolyplexes for the effective delivery of tumor suppressive mir-34a into triple-negative breast cancer cellsPoly-LysinePolyplexes RNATriple negative breast cancerMaterials Science(all)SDG 3 - Good Health and Well-beingPOCI-01-0145-FEDER-007265 UIDB/04077/2020 PTDC/QEQ-MED/2118/2014 2015 (IF/00007/2015) RTI2018-098027-B-C22Non-viral gene delivery using exogenous microRNAs is a potential strategy for fighting cancers with poor prognosis and which lack specific therapies, such as triple-negative breast cancer (TNBC). Herein we report the synthesis of six nontoxic electrostatic polymeric nanocapsules (P1 to P6) for microRNA delivery in TNBC cells. 1H Nuclear Magnetic Resonance (NMR) spectroscopy and Scanning Electron Microscopy (SEM) were used to characterize the nanopolyplexes, synthesized with Poly(L-Lysine) and hyaluronic acid (Ha). Studies on the activity of the ternary HA/PLI/miRNA-34 nanopolyplexes towards TNBC cell line MDA-MB-231 were conducted. The nanopolyplexes mediated intracellular restoration of tumor suppressor miR34a was evaluated by using Western blotting to quantify the expression level of the Bcl-2 protein. The results suggest that the P5, with a ratio PLI/Ha of 0.05, was the most promising for the delivery of miR-34a into TNBC cells; the P5 nanocapsules were able to reduce Bcl-2 expression at a protein level, and had an effect in the overall cell viability after 24 h treatment.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNDjafari, JamilaFernández-Lodeiro, JavierSantos, Hugo M.Lorenzo, JuliaRodriguez-Calado, SergiBértolo, EmiliaCapelo-Martínez, José LuisLodeiro, Carlos2021-05-06T22:44:02Z2020-11-242020-11-24T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://hdl.handle.net/10362/117271eng1996-1944PURE: 27428155https://doi.org/10.3390/ma13235309info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:00:24Zoai:run.unl.pt:10362/117271Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:34.618176Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Study and preparation of multifunctional poly(L-lysine)@hyaluronic acid nanopolyplexes for the effective delivery of tumor suppressive mir-34a into triple-negative breast cancer cells
title Study and preparation of multifunctional poly(L-lysine)@hyaluronic acid nanopolyplexes for the effective delivery of tumor suppressive mir-34a into triple-negative breast cancer cells
spellingShingle Study and preparation of multifunctional poly(L-lysine)@hyaluronic acid nanopolyplexes for the effective delivery of tumor suppressive mir-34a into triple-negative breast cancer cells
Djafari, Jamila
Poly-Lysine
Polyplexes RNA
Triple negative breast cancer
Materials Science(all)
SDG 3 - Good Health and Well-being
title_short Study and preparation of multifunctional poly(L-lysine)@hyaluronic acid nanopolyplexes for the effective delivery of tumor suppressive mir-34a into triple-negative breast cancer cells
title_full Study and preparation of multifunctional poly(L-lysine)@hyaluronic acid nanopolyplexes for the effective delivery of tumor suppressive mir-34a into triple-negative breast cancer cells
title_fullStr Study and preparation of multifunctional poly(L-lysine)@hyaluronic acid nanopolyplexes for the effective delivery of tumor suppressive mir-34a into triple-negative breast cancer cells
title_full_unstemmed Study and preparation of multifunctional poly(L-lysine)@hyaluronic acid nanopolyplexes for the effective delivery of tumor suppressive mir-34a into triple-negative breast cancer cells
title_sort Study and preparation of multifunctional poly(L-lysine)@hyaluronic acid nanopolyplexes for the effective delivery of tumor suppressive mir-34a into triple-negative breast cancer cells
author Djafari, Jamila
author_facet Djafari, Jamila
Fernández-Lodeiro, Javier
Santos, Hugo M.
Lorenzo, Julia
Rodriguez-Calado, Sergi
Bértolo, Emilia
Capelo-Martínez, José Luis
Lodeiro, Carlos
author_role author
author2 Fernández-Lodeiro, Javier
Santos, Hugo M.
Lorenzo, Julia
Rodriguez-Calado, Sergi
Bértolo, Emilia
Capelo-Martínez, José Luis
Lodeiro, Carlos
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Djafari, Jamila
Fernández-Lodeiro, Javier
Santos, Hugo M.
Lorenzo, Julia
Rodriguez-Calado, Sergi
Bértolo, Emilia
Capelo-Martínez, José Luis
Lodeiro, Carlos
dc.subject.por.fl_str_mv Poly-Lysine
Polyplexes RNA
Triple negative breast cancer
Materials Science(all)
SDG 3 - Good Health and Well-being
topic Poly-Lysine
Polyplexes RNA
Triple negative breast cancer
Materials Science(all)
SDG 3 - Good Health and Well-being
description POCI-01-0145-FEDER-007265 UIDB/04077/2020 PTDC/QEQ-MED/2118/2014 2015 (IF/00007/2015) RTI2018-098027-B-C22
publishDate 2020
dc.date.none.fl_str_mv 2020-11-24
2020-11-24T00:00:00Z
2021-05-06T22:44:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/117271
url http://hdl.handle.net/10362/117271
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1996-1944
PURE: 27428155
https://doi.org/10.3390/ma13235309
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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