Dynamic association of RNA-editing enzymes with the nucleolus
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2003 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/49437 |
Resumo: | © The Company of Biologists Limited 2003 |
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Dynamic association of RNA-editing enzymes with the nucleolusRNA editingADAR1ADAR2Nucleolus© The Company of Biologists Limited 2003ADAR1 and ADAR2 are editing enzymes that deaminate adenosine to inosine in long double stranded RNA duplexes and specific pre-mRNA transcripts. Here, we show that full-length and N-terminally truncated forms of ADAR1 are simultaneously expressed in HeLa and COS7 cells owing to the usage of alternative starting methionines. Because the N-terminus of ADAR1 contains a nuclear export signal, the full-length protein localizes predominantly in the cytoplasm, whereas the N-terminally truncated forms are exclusively nuclear and accumulate in the nucleolus. ADAR2, which lacks a region homologous to the N-terminal domain of ADAR1, localizes exclusively to the nucleus and similarly accumulates in the nucleolus. Within the nucleolus, ADAR1 and ADAR2 co-localize in a novel compartment. Photobleaching experiments demonstrate that, in live cells, ADAR1 and ADAR2 are in constant flux in and out of the nucleolus. When cells express the editing-competent glutamate receptor GluR-B RNA, endogenous ADAR1 and ADAR2 de-localize from the nucleolus and accumulate at sites where the substrate transcripts accumulate. This suggests that ADAR1 and ADAR2 are constantly moving through the nucleolus and might be recruited onto specific editing substrates present elsewhere in the cell.This study was supported by grants from Fundação para a Ciência e Tecnologia,Portugal, and the European Commission (QLG2-CT-2001-01554). This work was also supported by the MRC, a grant from the British Heart Foundation (PG/98086),Ministerio de Ciencia y Tecnologia of Spain (BFI2002-00454) and Fundación Marqués de Valdecilla' of Santander, Spain (A05/02). J.M.P.D. was supported by a long-term fellowship of the European Molecular Biology Organization (EMBO-ALTF 239-2000).The Company of BiologistsRepositório da Universidade de LisboaDesterro, JoanaKeegan, Liam P.Lafarga, MiguelBerciano, Maria TeresaO'Connell, MaryCarmo-Fonseca, Maria2021-09-07T13:45:18Z20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49437engJ Cell Sci. 2003 May 1;116(Pt 9):1805-18180021-953310.1242/jcs.003711477-9137info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:53:13Zoai:repositorio.ul.pt:10451/49437Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:04.550686Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Dynamic association of RNA-editing enzymes with the nucleolus |
| title |
Dynamic association of RNA-editing enzymes with the nucleolus |
| spellingShingle |
Dynamic association of RNA-editing enzymes with the nucleolus Desterro, Joana RNA editing ADAR1 ADAR2 Nucleolus |
| title_short |
Dynamic association of RNA-editing enzymes with the nucleolus |
| title_full |
Dynamic association of RNA-editing enzymes with the nucleolus |
| title_fullStr |
Dynamic association of RNA-editing enzymes with the nucleolus |
| title_full_unstemmed |
Dynamic association of RNA-editing enzymes with the nucleolus |
| title_sort |
Dynamic association of RNA-editing enzymes with the nucleolus |
| author |
Desterro, Joana |
| author_facet |
Desterro, Joana Keegan, Liam P. Lafarga, Miguel Berciano, Maria Teresa O'Connell, Mary Carmo-Fonseca, Maria |
| author_role |
author |
| author2 |
Keegan, Liam P. Lafarga, Miguel Berciano, Maria Teresa O'Connell, Mary Carmo-Fonseca, Maria |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Desterro, Joana Keegan, Liam P. Lafarga, Miguel Berciano, Maria Teresa O'Connell, Mary Carmo-Fonseca, Maria |
| dc.subject.por.fl_str_mv |
RNA editing ADAR1 ADAR2 Nucleolus |
| topic |
RNA editing ADAR1 ADAR2 Nucleolus |
| description |
© The Company of Biologists Limited 2003 |
| publishDate |
2003 |
| dc.date.none.fl_str_mv |
2003 2003-01-01T00:00:00Z 2021-09-07T13:45:18Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/49437 |
| url |
http://hdl.handle.net/10451/49437 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
J Cell Sci. 2003 May 1;116(Pt 9):1805-1818 0021-9533 10.1242/jcs.00371 1477-9137 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
The Company of Biologists |
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The Company of Biologists |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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