Cytotoxicity of Portuguese propolis: the proximity of the in vitro doses for tumor and normal cell lines

Detalhes bibliográficos
Autor(a) principal: Calhelha, Ricardo C.
Data de Publicação: 2014
Outros Autores: Falcão, Soraia, Queiroz, Maria João R. P., Vilas-Boas, Miguel, Ferreira, Isabel C. F. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/64518
Resumo: With a complex chemical composition rich in phenolic compounds, propolis (resinous substance collected by Apis mellifera from various tree buds) exhibits a broad spectrum of biological activities. Recently, in vitro and in vivo data suggest that propolis has anticancer properties, but is the cytoxicity of propolis specific for tumor cells? To answer this question, the cytotoxicity of phenolic extracts from Portuguese propolis of different origins was evaluated using human tumor cell lines (MCF7-breast adenocarcinoma, NCI-H460-non-small cell lung carcinoma, HCT15-colon carcinoma, HeLa-cervical carcinoma, and HepG2-hepatocellular carcinoma), and non-tumor primary cells (PLP2). The studied propolis presented high cytotoxic potential for human tumor cell lines, mostly for HCT15. Nevertheless, excluding HCT15 cell line, the extracts at the GI50 obtained for tumor cell lines showed, in general, cytotoxicity for normal cells (PLP2). Propolis phenolic extracts comprise phytochemicals that should be further studied for their bioactive properties against human colon carcinoma. In the other cases, the proximity of the in vitro cytotoxic doses for tumor and normal cell lines should be confirmed by in vivo tests and may highlight the need for selection of specific compounds within the propolis extract.
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spelling Cytotoxicity of Portuguese propolis: the proximity of the in vitro doses for tumor and normal cell linesCell DeathCell Line, TumorDose-Response Relationship, DrugFlavonoidsHumansPhenolsPortugalPropolisScience & TechnologyWith a complex chemical composition rich in phenolic compounds, propolis (resinous substance collected by Apis mellifera from various tree buds) exhibits a broad spectrum of biological activities. Recently, in vitro and in vivo data suggest that propolis has anticancer properties, but is the cytoxicity of propolis specific for tumor cells? To answer this question, the cytotoxicity of phenolic extracts from Portuguese propolis of different origins was evaluated using human tumor cell lines (MCF7-breast adenocarcinoma, NCI-H460-non-small cell lung carcinoma, HCT15-colon carcinoma, HeLa-cervical carcinoma, and HepG2-hepatocellular carcinoma), and non-tumor primary cells (PLP2). The studied propolis presented high cytotoxic potential for human tumor cell lines, mostly for HCT15. Nevertheless, excluding HCT15 cell line, the extracts at the GI50 obtained for tumor cell lines showed, in general, cytotoxicity for normal cells (PLP2). Propolis phenolic extracts comprise phytochemicals that should be further studied for their bioactive properties against human colon carcinoma. In the other cases, the proximity of the in vitro cytotoxic doses for tumor and normal cell lines should be confirmed by in vivo tests and may highlight the need for selection of specific compounds within the propolis extract.The authors are grateful to Foundation for Science and Technology (FCT), Portugal, for providing the financial support to the research centers CIMO (PEst-OE/AGR/UI0690/2011) and CQ-UM (PEst-C/QUI/UI0686/2011). Ricardo C. Calhelha thanks FCT, POPH-QREN, and FSE for his Grant (SFRH/BPD/68344/2010). Thanks are also due to the National Federation of Portuguese Beekeepers for supplying propolis samples.Hindawi LimitedUniversidade do MinhoCalhelha, Ricardo C.Falcão, SoraiaQueiroz, Maria João R. P.Vilas-Boas, MiguelFerreira, Isabel C. F. R.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/64518engCalhelha, Ricardo C.; Falcão, Soraia; Queiroz, Maria João R.P.; Vilas-Boas, Miguel; Ferreira, Isabel C.F.R. (2014). Cytotoxicity of portuguese propolis: the proximity of the in vitro doses for tumor and normal cell lines. BioMed Research International. ISSN 2314-6141. p. 1-72314-613310.1155/2014/89736124982911https://www.hindawi.com/journals/bmri/2014/897361/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:09Zoai:repositorium.sdum.uminho.pt:1822/64518Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:21.282929Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cytotoxicity of Portuguese propolis: the proximity of the in vitro doses for tumor and normal cell lines
title Cytotoxicity of Portuguese propolis: the proximity of the in vitro doses for tumor and normal cell lines
spellingShingle Cytotoxicity of Portuguese propolis: the proximity of the in vitro doses for tumor and normal cell lines
Calhelha, Ricardo C.
Cell Death
Cell Line, Tumor
Dose-Response Relationship, Drug
Flavonoids
Humans
Phenols
Portugal
Propolis
Science & Technology
title_short Cytotoxicity of Portuguese propolis: the proximity of the in vitro doses for tumor and normal cell lines
title_full Cytotoxicity of Portuguese propolis: the proximity of the in vitro doses for tumor and normal cell lines
title_fullStr Cytotoxicity of Portuguese propolis: the proximity of the in vitro doses for tumor and normal cell lines
title_full_unstemmed Cytotoxicity of Portuguese propolis: the proximity of the in vitro doses for tumor and normal cell lines
title_sort Cytotoxicity of Portuguese propolis: the proximity of the in vitro doses for tumor and normal cell lines
author Calhelha, Ricardo C.
author_facet Calhelha, Ricardo C.
Falcão, Soraia
Queiroz, Maria João R. P.
Vilas-Boas, Miguel
Ferreira, Isabel C. F. R.
author_role author
author2 Falcão, Soraia
Queiroz, Maria João R. P.
Vilas-Boas, Miguel
Ferreira, Isabel C. F. R.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Calhelha, Ricardo C.
Falcão, Soraia
Queiroz, Maria João R. P.
Vilas-Boas, Miguel
Ferreira, Isabel C. F. R.
dc.subject.por.fl_str_mv Cell Death
Cell Line, Tumor
Dose-Response Relationship, Drug
Flavonoids
Humans
Phenols
Portugal
Propolis
Science & Technology
topic Cell Death
Cell Line, Tumor
Dose-Response Relationship, Drug
Flavonoids
Humans
Phenols
Portugal
Propolis
Science & Technology
description With a complex chemical composition rich in phenolic compounds, propolis (resinous substance collected by Apis mellifera from various tree buds) exhibits a broad spectrum of biological activities. Recently, in vitro and in vivo data suggest that propolis has anticancer properties, but is the cytoxicity of propolis specific for tumor cells? To answer this question, the cytotoxicity of phenolic extracts from Portuguese propolis of different origins was evaluated using human tumor cell lines (MCF7-breast adenocarcinoma, NCI-H460-non-small cell lung carcinoma, HCT15-colon carcinoma, HeLa-cervical carcinoma, and HepG2-hepatocellular carcinoma), and non-tumor primary cells (PLP2). The studied propolis presented high cytotoxic potential for human tumor cell lines, mostly for HCT15. Nevertheless, excluding HCT15 cell line, the extracts at the GI50 obtained for tumor cell lines showed, in general, cytotoxicity for normal cells (PLP2). Propolis phenolic extracts comprise phytochemicals that should be further studied for their bioactive properties against human colon carcinoma. In the other cases, the proximity of the in vitro cytotoxic doses for tumor and normal cell lines should be confirmed by in vivo tests and may highlight the need for selection of specific compounds within the propolis extract.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/64518
url http://hdl.handle.net/1822/64518
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Calhelha, Ricardo C.; Falcão, Soraia; Queiroz, Maria João R.P.; Vilas-Boas, Miguel; Ferreira, Isabel C.F.R. (2014). Cytotoxicity of portuguese propolis: the proximity of the in vitro doses for tumor and normal cell lines. BioMed Research International. ISSN 2314-6141. p. 1-7
2314-6133
10.1155/2014/897361
24982911
https://www.hindawi.com/journals/bmri/2014/897361/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi Limited
publisher.none.fl_str_mv Hindawi Limited
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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