Development of germplasm cryopreservation techniques for honey bee conservation
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10348/10720 |
Resumo: | Cryopreservation is a recent tools that has enormous potential in all aspects of human life. With this tool, the limitations for preserving genetic material become almost impossible to grasp. With this dissertation there in an attempt to understand better how this new technology works and how to put it to good use in practical manners regarding honey bee conservation. This dissertation has two parts, the theoretical part and the practical part. The practical part was only possible to do in Länderinstitut für Bienenkunde, Berlin. When it comes to results found in this dissertation, data suggests that there is a better time for the collection of semen samples that obtain better results when put through cryopreservation protocols. We can also affirm that there is a possibility of sending semen samples by mail. The last conclusion regarding sperm analyses is that, there is no relationship between inbreeding factors and semen quality. Regarding the RNA experiments, even though they present to be a solid foundation for later experiments, by their preliminary nature, there is no definitive conclusion to be taken from them. |
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Development of germplasm cryopreservation techniques for honey bee conservationHoney beeCryopreservationCryopreservation is a recent tools that has enormous potential in all aspects of human life. With this tool, the limitations for preserving genetic material become almost impossible to grasp. With this dissertation there in an attempt to understand better how this new technology works and how to put it to good use in practical manners regarding honey bee conservation. This dissertation has two parts, the theoretical part and the practical part. The practical part was only possible to do in Länderinstitut für Bienenkunde, Berlin. When it comes to results found in this dissertation, data suggests that there is a better time for the collection of semen samples that obtain better results when put through cryopreservation protocols. We can also affirm that there is a possibility of sending semen samples by mail. The last conclusion regarding sperm analyses is that, there is no relationship between inbreeding factors and semen quality. Regarding the RNA experiments, even though they present to be a solid foundation for later experiments, by their preliminary nature, there is no definitive conclusion to be taken from them.A criopreservação é uma ferramenta relativamente recente com um enorme potencial em todos os aspetos da vida humana. Com esta ferramenta, as limitações no que toca a preservação de material genético tornam-se impossíveis de visualizar. Com esta dissertação á uma tentativa de melhor entender como esta tecnologia funciona e como a por em pratica no que toca a preservação da abelha. Esta dissertação é constituída por duas partes, a parte teórica e a parte pratica. A parte pratica teve de ser realizada Länderinstitut für Bienenkunde em Berlim. No que toca aos resultados desta experiencia, os dados recolhidos sugerem existe uma altura em que a recolha de amostra de sémen se traduz em melhores resultados quando exposta a protocolos de criopreservação. Também podemos afirmar que existe a possibilidade de transporte do sémen através do correio. Por último, podemos concluir não existe uma ligação entre factor de consanguinidade e qualidade do sémen. Relativamente aos ensaios com recurso a RNA, podemos afirmar que estes constituem uma base sólida para experiências futuras embora, não seja possível retirar conclusões definitas devido á sua natureza extremamente preliminar do estudo.2021-10-04T15:38:50Z2020-06-06T00:00:00Z2020-06-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/10348/10720engPereira, Catarina Margarida Videira Diasinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-02T12:29:08Zoai:repositorio.utad.pt:10348/10720Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:00:20.987292Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development of germplasm cryopreservation techniques for honey bee conservation |
title |
Development of germplasm cryopreservation techniques for honey bee conservation |
spellingShingle |
Development of germplasm cryopreservation techniques for honey bee conservation Pereira, Catarina Margarida Videira Dias Honey bee Cryopreservation |
title_short |
Development of germplasm cryopreservation techniques for honey bee conservation |
title_full |
Development of germplasm cryopreservation techniques for honey bee conservation |
title_fullStr |
Development of germplasm cryopreservation techniques for honey bee conservation |
title_full_unstemmed |
Development of germplasm cryopreservation techniques for honey bee conservation |
title_sort |
Development of germplasm cryopreservation techniques for honey bee conservation |
author |
Pereira, Catarina Margarida Videira Dias |
author_facet |
Pereira, Catarina Margarida Videira Dias |
author_role |
author |
dc.contributor.author.fl_str_mv |
Pereira, Catarina Margarida Videira Dias |
dc.subject.por.fl_str_mv |
Honey bee Cryopreservation |
topic |
Honey bee Cryopreservation |
description |
Cryopreservation is a recent tools that has enormous potential in all aspects of human life. With this tool, the limitations for preserving genetic material become almost impossible to grasp. With this dissertation there in an attempt to understand better how this new technology works and how to put it to good use in practical manners regarding honey bee conservation. This dissertation has two parts, the theoretical part and the practical part. The practical part was only possible to do in Länderinstitut für Bienenkunde, Berlin. When it comes to results found in this dissertation, data suggests that there is a better time for the collection of semen samples that obtain better results when put through cryopreservation protocols. We can also affirm that there is a possibility of sending semen samples by mail. The last conclusion regarding sperm analyses is that, there is no relationship between inbreeding factors and semen quality. Regarding the RNA experiments, even though they present to be a solid foundation for later experiments, by their preliminary nature, there is no definitive conclusion to be taken from them. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-06T00:00:00Z 2020-06-06 2021-10-04T15:38:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10348/10720 |
url |
http://hdl.handle.net/10348/10720 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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