Engineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene delivery
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/4084 |
Resumo: | ABSTRACT: Carbon dots (CDs) and G4-G6 (polyamidoamine)- PAMAM-NH2 dendrimers were self-assembled to produce CDs@ PAMAM nanohybrids for transfection and bioimaging purposes. CDs were synthesized by the hydrothermal method, using ascorbic acid as a starting precursor and characterized by transmission electron microscopy, UV−Vis, and fluorescence (in solution and solid-state) techniques. CDs were electrostatically combined with PAMAM dendrimers at room temperature, and the UV−Vis, fluorescence, and NMR spectroscopies were used to confirm the self-assembly. When compared to pristine CDs, nanohybrids were more photostable, resisting high acidic and basic pH. Moreover, they were considerably internalized by cells, as assessed by flow cytometry and fluorescence microscopy, and, when excited, displayed multi color emission easily quantified and visualized. These nanoscale hybrids, coined hybridplexes, can condense pDNA and transfecting cells successfully, particularly the G5 CDs@PAMAM nanohybrids. In summary, CDs prepared in mild and smooth lab conditions, showing good optical properties, were used to prepare elegantly CDs@PAMAM nanohybrids with promising biomedical applications. |
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Engineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene deliveryCarbon dotsG4-G6PAMAM dendrimerPAMAM nanohybridsEngineered fluorescentBioimagingGene delivery.Faculdade de Ciências Exatas e da EngenhariaCentro de Química da MadeiraABSTRACT: Carbon dots (CDs) and G4-G6 (polyamidoamine)- PAMAM-NH2 dendrimers were self-assembled to produce CDs@ PAMAM nanohybrids for transfection and bioimaging purposes. CDs were synthesized by the hydrothermal method, using ascorbic acid as a starting precursor and characterized by transmission electron microscopy, UV−Vis, and fluorescence (in solution and solid-state) techniques. CDs were electrostatically combined with PAMAM dendrimers at room temperature, and the UV−Vis, fluorescence, and NMR spectroscopies were used to confirm the self-assembly. When compared to pristine CDs, nanohybrids were more photostable, resisting high acidic and basic pH. Moreover, they were considerably internalized by cells, as assessed by flow cytometry and fluorescence microscopy, and, when excited, displayed multi color emission easily quantified and visualized. These nanoscale hybrids, coined hybridplexes, can condense pDNA and transfecting cells successfully, particularly the G5 CDs@PAMAM nanohybrids. In summary, CDs prepared in mild and smooth lab conditions, showing good optical properties, were used to prepare elegantly CDs@PAMAM nanohybrids with promising biomedical applications.American Chemical SocietyDigitUMaMartins, IvoTomás, HelenaLahoz, FernandoRodrigues, João2022-06-01T00:30:29Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/4084engMartins, I., Tomás, H., Lahoz, F., & Rodrigues, J. (2021). Engineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene delivery. Biomacromolecules, 22(6), 2436-2450. DOI: 10.1021/acs.biomac.1c0023210.1021/acs.biomac.1c00232info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-17T05:58:02Zoai:digituma.uma.pt:10400.13/4084Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:07:54.544132Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Engineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene delivery |
title |
Engineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene delivery |
spellingShingle |
Engineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene delivery Martins, Ivo Carbon dots G4-G6 PAMAM dendrimer PAMAM nanohybrids Engineered fluorescent Bioimaging Gene delivery . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
title_short |
Engineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene delivery |
title_full |
Engineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene delivery |
title_fullStr |
Engineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene delivery |
title_full_unstemmed |
Engineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene delivery |
title_sort |
Engineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene delivery |
author |
Martins, Ivo |
author_facet |
Martins, Ivo Tomás, Helena Lahoz, Fernando Rodrigues, João |
author_role |
author |
author2 |
Tomás, Helena Lahoz, Fernando Rodrigues, João |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Martins, Ivo Tomás, Helena Lahoz, Fernando Rodrigues, João |
dc.subject.por.fl_str_mv |
Carbon dots G4-G6 PAMAM dendrimer PAMAM nanohybrids Engineered fluorescent Bioimaging Gene delivery . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
topic |
Carbon dots G4-G6 PAMAM dendrimer PAMAM nanohybrids Engineered fluorescent Bioimaging Gene delivery . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
description |
ABSTRACT: Carbon dots (CDs) and G4-G6 (polyamidoamine)- PAMAM-NH2 dendrimers were self-assembled to produce CDs@ PAMAM nanohybrids for transfection and bioimaging purposes. CDs were synthesized by the hydrothermal method, using ascorbic acid as a starting precursor and characterized by transmission electron microscopy, UV−Vis, and fluorescence (in solution and solid-state) techniques. CDs were electrostatically combined with PAMAM dendrimers at room temperature, and the UV−Vis, fluorescence, and NMR spectroscopies were used to confirm the self-assembly. When compared to pristine CDs, nanohybrids were more photostable, resisting high acidic and basic pH. Moreover, they were considerably internalized by cells, as assessed by flow cytometry and fluorescence microscopy, and, when excited, displayed multi color emission easily quantified and visualized. These nanoscale hybrids, coined hybridplexes, can condense pDNA and transfecting cells successfully, particularly the G5 CDs@PAMAM nanohybrids. In summary, CDs prepared in mild and smooth lab conditions, showing good optical properties, were used to prepare elegantly CDs@PAMAM nanohybrids with promising biomedical applications. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z 2022-06-01T00:30:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/4084 |
url |
http://hdl.handle.net/10400.13/4084 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Martins, I., Tomás, H., Lahoz, F., & Rodrigues, J. (2021). Engineered fluorescent carbon dots and G4-G6 PAMAM dendrimer nanohybrids for bioimaging and gene delivery. Biomacromolecules, 22(6), 2436-2450. DOI: 10.1021/acs.biomac.1c00232 10.1021/acs.biomac.1c00232 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1817554486497902592 |