D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/16573 https://doi.org/http://dx.doi.org/10.4149/av_2015_02_185 https://doi.org/10.4149/av_2015_02_185 |
Resumo: | Field sweet potato plants showing virus-like symptoms, as stunting, leaf distortion, mosaic and chlorosis, were collected in southwest Portugal and tested for the presence of four potyviruses, sweet potato virus C (SPVC), sweet potato virus 2 (SPV2), sweet potato feathery mottle virus (SPFMV), sweet potato virus G (SPVG), and the crinivirus sweet potato chlorotic stunt virus (SPCSV). DsRNA fractions were extracted from symptomatic leaves and used as templates in single and multiplex RT-PCR assays using previously described specific primers for each analyzed virus. The amplified reaction products for SPVC, SPV2 and SPFMV were of expected size, and direct sequencing of PCR products revealed that they correspond to the coat protein gene (CP) and showed 98%, 99% and 99% identity, respectively, to those viruses. Comparison of the CP genomic and amino acid sequences of the Portuguese viral isolates recovered here with those of ten other sequences of isolates obtained in different countries retrieved from the GenBank showed very few differences. The application of the RT-PCR assays revealed for the first time the presence of SPVC and SPFMV in the sweet potato crop in Portugal, the absence of SPVG and SPCSV in tested plants, as well as the occurrence of triple virus infections under field conditions. |
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D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in PortugalSweet potatoVirusesPortugalField sweet potato plants showing virus-like symptoms, as stunting, leaf distortion, mosaic and chlorosis, were collected in southwest Portugal and tested for the presence of four potyviruses, sweet potato virus C (SPVC), sweet potato virus 2 (SPV2), sweet potato feathery mottle virus (SPFMV), sweet potato virus G (SPVG), and the crinivirus sweet potato chlorotic stunt virus (SPCSV). DsRNA fractions were extracted from symptomatic leaves and used as templates in single and multiplex RT-PCR assays using previously described specific primers for each analyzed virus. The amplified reaction products for SPVC, SPV2 and SPFMV were of expected size, and direct sequencing of PCR products revealed that they correspond to the coat protein gene (CP) and showed 98%, 99% and 99% identity, respectively, to those viruses. Comparison of the CP genomic and amino acid sequences of the Portuguese viral isolates recovered here with those of ten other sequences of isolates obtained in different countries retrieved from the GenBank showed very few differences. The application of the RT-PCR assays revealed for the first time the presence of SPVC and SPFMV in the sweet potato crop in Portugal, the absence of SPVG and SPCSV in tested plants, as well as the occurrence of triple virus infections under field conditions.2016-01-07T10:49:19Z2016-01-072015-05-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/16573https://doi.org/http://dx.doi.org/10.4149/av_2015_02_185http://hdl.handle.net/10174/16573https://doi.org/10.4149/av_2015_02_185porVaranda et al.,2015 Acta VirologicaICAAMcarlavaranda@uevora.ptsu_santos12@hotmail.commonicadmportella@hotmail.comiclara@uevora.ptmrff@uevora.pt581Varanda, CarlaSantos, SusanaOliveira, MônicaClara, M. Ivone E.Félix, M. Rosárioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:03:27Zoai:dspace.uevora.pt:10174/16573Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:08:56.478844Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal |
title |
D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal |
spellingShingle |
D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal Varanda, Carla Sweet potato Viruses Portugal |
title_short |
D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal |
title_full |
D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal |
title_fullStr |
D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal |
title_full_unstemmed |
D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal |
title_sort |
D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal |
author |
Varanda, Carla |
author_facet |
Varanda, Carla Santos, Susana Oliveira, Mônica Clara, M. Ivone E. Félix, M. Rosário |
author_role |
author |
author2 |
Santos, Susana Oliveira, Mônica Clara, M. Ivone E. Félix, M. Rosário |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Varanda, Carla Santos, Susana Oliveira, Mônica Clara, M. Ivone E. Félix, M. Rosário |
dc.subject.por.fl_str_mv |
Sweet potato Viruses Portugal |
topic |
Sweet potato Viruses Portugal |
description |
Field sweet potato plants showing virus-like symptoms, as stunting, leaf distortion, mosaic and chlorosis, were collected in southwest Portugal and tested for the presence of four potyviruses, sweet potato virus C (SPVC), sweet potato virus 2 (SPV2), sweet potato feathery mottle virus (SPFMV), sweet potato virus G (SPVG), and the crinivirus sweet potato chlorotic stunt virus (SPCSV). DsRNA fractions were extracted from symptomatic leaves and used as templates in single and multiplex RT-PCR assays using previously described specific primers for each analyzed virus. The amplified reaction products for SPVC, SPV2 and SPFMV were of expected size, and direct sequencing of PCR products revealed that they correspond to the coat protein gene (CP) and showed 98%, 99% and 99% identity, respectively, to those viruses. Comparison of the CP genomic and amino acid sequences of the Portuguese viral isolates recovered here with those of ten other sequences of isolates obtained in different countries retrieved from the GenBank showed very few differences. The application of the RT-PCR assays revealed for the first time the presence of SPVC and SPFMV in the sweet potato crop in Portugal, the absence of SPVG and SPCSV in tested plants, as well as the occurrence of triple virus infections under field conditions. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-05-12T00:00:00Z 2016-01-07T10:49:19Z 2016-01-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/16573 https://doi.org/http://dx.doi.org/10.4149/av_2015_02_185 http://hdl.handle.net/10174/16573 https://doi.org/10.4149/av_2015_02_185 |
url |
http://hdl.handle.net/10174/16573 https://doi.org/http://dx.doi.org/10.4149/av_2015_02_185 https://doi.org/10.4149/av_2015_02_185 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Varanda et al.,2015 Acta Virologica ICAAM carlavaranda@uevora.pt su_santos12@hotmail.com monicadmportella@hotmail.com iclara@uevora.pt mrff@uevora.pt 581 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136572398895104 |