D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal

Detalhes bibliográficos
Autor(a) principal: Varanda, Carla
Data de Publicação: 2015
Outros Autores: Santos, Susana, Oliveira, Mônica, Clara, M. Ivone E., Félix, M. Rosário
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/16573
https://doi.org/http://dx.doi.org/10.4149/av_2015_02_185
https://doi.org/10.4149/av_2015_02_185
Resumo: Field sweet potato plants showing virus-like symptoms, as stunting, leaf distortion, mosaic and chlorosis, were collected in southwest Portugal and tested for the presence of four potyviruses, sweet potato virus C (SPVC), sweet potato virus 2 (SPV2), sweet potato feathery mottle virus (SPFMV), sweet potato virus G (SPVG), and the crinivirus sweet potato chlorotic stunt virus (SPCSV). DsRNA fractions were extracted from symptomatic leaves and used as templates in single and multiplex RT-PCR assays using previously described specific primers for each analyzed virus. The amplified reaction products for SPVC, SPV2 and SPFMV were of expected size, and direct sequencing of PCR products revealed that they correspond to the coat protein gene (CP) and showed 98%, 99% and 99% identity, respectively, to those viruses. Comparison of the CP genomic and amino acid sequences of the Portuguese viral isolates recovered here with those of ten other sequences of isolates obtained in different countries retrieved from the GenBank showed very few differences. The application of the RT-PCR assays revealed for the first time the presence of SPVC and SPFMV in the sweet potato crop in Portugal, the absence of SPVG and SPCSV in tested plants, as well as the occurrence of triple virus infections under field conditions.
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spelling D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in PortugalSweet potatoVirusesPortugalField sweet potato plants showing virus-like symptoms, as stunting, leaf distortion, mosaic and chlorosis, were collected in southwest Portugal and tested for the presence of four potyviruses, sweet potato virus C (SPVC), sweet potato virus 2 (SPV2), sweet potato feathery mottle virus (SPFMV), sweet potato virus G (SPVG), and the crinivirus sweet potato chlorotic stunt virus (SPCSV). DsRNA fractions were extracted from symptomatic leaves and used as templates in single and multiplex RT-PCR assays using previously described specific primers for each analyzed virus. The amplified reaction products for SPVC, SPV2 and SPFMV were of expected size, and direct sequencing of PCR products revealed that they correspond to the coat protein gene (CP) and showed 98%, 99% and 99% identity, respectively, to those viruses. Comparison of the CP genomic and amino acid sequences of the Portuguese viral isolates recovered here with those of ten other sequences of isolates obtained in different countries retrieved from the GenBank showed very few differences. The application of the RT-PCR assays revealed for the first time the presence of SPVC and SPFMV in the sweet potato crop in Portugal, the absence of SPVG and SPCSV in tested plants, as well as the occurrence of triple virus infections under field conditions.2016-01-07T10:49:19Z2016-01-072015-05-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/16573https://doi.org/http://dx.doi.org/10.4149/av_2015_02_185http://hdl.handle.net/10174/16573https://doi.org/10.4149/av_2015_02_185porVaranda et al.,2015 Acta VirologicaICAAMcarlavaranda@uevora.ptsu_santos12@hotmail.commonicadmportella@hotmail.comiclara@uevora.ptmrff@uevora.pt581Varanda, CarlaSantos, SusanaOliveira, MônicaClara, M. Ivone E.Félix, M. Rosárioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:03:27Zoai:dspace.uevora.pt:10174/16573Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:08:56.478844Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal
title D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal
spellingShingle D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal
Varanda, Carla
Sweet potato
Viruses
Portugal
title_short D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal
title_full D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal
title_fullStr D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal
title_full_unstemmed D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal
title_sort D etection of sweet potato virus C, sweet potato virus 2 and sweet potato feathery mottle virus in Portugal
author Varanda, Carla
author_facet Varanda, Carla
Santos, Susana
Oliveira, Mônica
Clara, M. Ivone E.
Félix, M. Rosário
author_role author
author2 Santos, Susana
Oliveira, Mônica
Clara, M. Ivone E.
Félix, M. Rosário
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Varanda, Carla
Santos, Susana
Oliveira, Mônica
Clara, M. Ivone E.
Félix, M. Rosário
dc.subject.por.fl_str_mv Sweet potato
Viruses
Portugal
topic Sweet potato
Viruses
Portugal
description Field sweet potato plants showing virus-like symptoms, as stunting, leaf distortion, mosaic and chlorosis, were collected in southwest Portugal and tested for the presence of four potyviruses, sweet potato virus C (SPVC), sweet potato virus 2 (SPV2), sweet potato feathery mottle virus (SPFMV), sweet potato virus G (SPVG), and the crinivirus sweet potato chlorotic stunt virus (SPCSV). DsRNA fractions were extracted from symptomatic leaves and used as templates in single and multiplex RT-PCR assays using previously described specific primers for each analyzed virus. The amplified reaction products for SPVC, SPV2 and SPFMV were of expected size, and direct sequencing of PCR products revealed that they correspond to the coat protein gene (CP) and showed 98%, 99% and 99% identity, respectively, to those viruses. Comparison of the CP genomic and amino acid sequences of the Portuguese viral isolates recovered here with those of ten other sequences of isolates obtained in different countries retrieved from the GenBank showed very few differences. The application of the RT-PCR assays revealed for the first time the presence of SPVC and SPFMV in the sweet potato crop in Portugal, the absence of SPVG and SPCSV in tested plants, as well as the occurrence of triple virus infections under field conditions.
publishDate 2015
dc.date.none.fl_str_mv 2015-05-12T00:00:00Z
2016-01-07T10:49:19Z
2016-01-07
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/16573
https://doi.org/http://dx.doi.org/10.4149/av_2015_02_185
http://hdl.handle.net/10174/16573
https://doi.org/10.4149/av_2015_02_185
url http://hdl.handle.net/10174/16573
https://doi.org/http://dx.doi.org/10.4149/av_2015_02_185
https://doi.org/10.4149/av_2015_02_185
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Varanda et al.,2015 Acta Virologica
ICAAM
carlavaranda@uevora.pt
su_santos12@hotmail.com
monicadmportella@hotmail.com
iclara@uevora.pt
mrff@uevora.pt
581
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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