Inhibitory effect of 5-aminoimidazole-4-carbohydrazonamides derivatives against Candida spp. biofilm on nanohydroxyapatite substrate
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10284/8228 |
Resumo: | Candida can adhere and form biofilm on biomaterials commonly used in medical devices which is a key attribute that enhances its ability to cause infections in humans. Furthermore, biomaterial-related infections represent a major therapeutic challenge since Candida biofilms are implicated in antifungal therapies failure. The goals of the present work were to investigate the effect of three 5-aminoimidazole-4-carbohydrazonamides, namely (Z)-5-amino-1-methyl-N'-aryl-1H-imidazole-4-carbohydrazonamides [aryl = phenyl (1a), 4-fluorophenyl (1b), 3-fluorophenyl (1c)], on Candida albicans and Candida krusei biofilm on nanohydroxyapatite substrate, a well-known bioactive ceramic material. To address these goals, both quantitative methods (by cultivable cell numbers) and qualitative evaluation (by scanning electron microscopy) were used. Compounds cytocompatibility towards osteoblast-like cells was also evaluated after 24 h of exposure, through resazurin assay. The three tested compounds displayed a strong inhibitory effect on biofilm development of both Candida species as potent in vitro activity against C. albicans sessile cells. Regarding cytocompatibility, a concentration-dependent effect was observed. Together, these findings indicated that the potent activity of imidazole derivatives on Candida spp. biofilms on nanohydroxyapatite substrate, in particular compound 1c, is worth further investigating. |
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Inhibitory effect of 5-aminoimidazole-4-carbohydrazonamides derivatives against Candida spp. biofilm on nanohydroxyapatite substrateBiomaterialsBiofilmsCandida can adhere and form biofilm on biomaterials commonly used in medical devices which is a key attribute that enhances its ability to cause infections in humans. Furthermore, biomaterial-related infections represent a major therapeutic challenge since Candida biofilms are implicated in antifungal therapies failure. The goals of the present work were to investigate the effect of three 5-aminoimidazole-4-carbohydrazonamides, namely (Z)-5-amino-1-methyl-N'-aryl-1H-imidazole-4-carbohydrazonamides [aryl = phenyl (1a), 4-fluorophenyl (1b), 3-fluorophenyl (1c)], on Candida albicans and Candida krusei biofilm on nanohydroxyapatite substrate, a well-known bioactive ceramic material. To address these goals, both quantitative methods (by cultivable cell numbers) and qualitative evaluation (by scanning electron microscopy) were used. Compounds cytocompatibility towards osteoblast-like cells was also evaluated after 24 h of exposure, through resazurin assay. The three tested compounds displayed a strong inhibitory effect on biofilm development of both Candida species as potent in vitro activity against C. albicans sessile cells. Regarding cytocompatibility, a concentration-dependent effect was observed. Together, these findings indicated that the potent activity of imidazole derivatives on Candida spp. biofilms on nanohydroxyapatite substrate, in particular compound 1c, is worth further investigating.Springer VerlagRepositório Institucional da Universidade Fernando PessoaGabriel, C.Grenho, L.Cerqueira, FátimaMedeiros, R.Dias, A. M.Ribeiro, A. I.Proença, M. F.Fernandes, M. H.Sousa, J. C.Monteiro, F. J.Ferraz, M. P.2019-01-01T00:00:00Z2019-01-01T00:00:00Z2029-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10284/8228eng0301-486X10.1007/s11046-019-00400-4info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-06T02:07:37Zoai:bdigital.ufp.pt:10284/8228Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:45:06.453553Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Inhibitory effect of 5-aminoimidazole-4-carbohydrazonamides derivatives against Candida spp. biofilm on nanohydroxyapatite substrate |
title |
Inhibitory effect of 5-aminoimidazole-4-carbohydrazonamides derivatives against Candida spp. biofilm on nanohydroxyapatite substrate |
spellingShingle |
Inhibitory effect of 5-aminoimidazole-4-carbohydrazonamides derivatives against Candida spp. biofilm on nanohydroxyapatite substrate Gabriel, C. Biomaterials Biofilms |
title_short |
Inhibitory effect of 5-aminoimidazole-4-carbohydrazonamides derivatives against Candida spp. biofilm on nanohydroxyapatite substrate |
title_full |
Inhibitory effect of 5-aminoimidazole-4-carbohydrazonamides derivatives against Candida spp. biofilm on nanohydroxyapatite substrate |
title_fullStr |
Inhibitory effect of 5-aminoimidazole-4-carbohydrazonamides derivatives against Candida spp. biofilm on nanohydroxyapatite substrate |
title_full_unstemmed |
Inhibitory effect of 5-aminoimidazole-4-carbohydrazonamides derivatives against Candida spp. biofilm on nanohydroxyapatite substrate |
title_sort |
Inhibitory effect of 5-aminoimidazole-4-carbohydrazonamides derivatives against Candida spp. biofilm on nanohydroxyapatite substrate |
author |
Gabriel, C. |
author_facet |
Gabriel, C. Grenho, L. Cerqueira, Fátima Medeiros, R. Dias, A. M. Ribeiro, A. I. Proença, M. F. Fernandes, M. H. Sousa, J. C. Monteiro, F. J. Ferraz, M. P. |
author_role |
author |
author2 |
Grenho, L. Cerqueira, Fátima Medeiros, R. Dias, A. M. Ribeiro, A. I. Proença, M. F. Fernandes, M. H. Sousa, J. C. Monteiro, F. J. Ferraz, M. P. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Institucional da Universidade Fernando Pessoa |
dc.contributor.author.fl_str_mv |
Gabriel, C. Grenho, L. Cerqueira, Fátima Medeiros, R. Dias, A. M. Ribeiro, A. I. Proença, M. F. Fernandes, M. H. Sousa, J. C. Monteiro, F. J. Ferraz, M. P. |
dc.subject.por.fl_str_mv |
Biomaterials Biofilms |
topic |
Biomaterials Biofilms |
description |
Candida can adhere and form biofilm on biomaterials commonly used in medical devices which is a key attribute that enhances its ability to cause infections in humans. Furthermore, biomaterial-related infections represent a major therapeutic challenge since Candida biofilms are implicated in antifungal therapies failure. The goals of the present work were to investigate the effect of three 5-aminoimidazole-4-carbohydrazonamides, namely (Z)-5-amino-1-methyl-N'-aryl-1H-imidazole-4-carbohydrazonamides [aryl = phenyl (1a), 4-fluorophenyl (1b), 3-fluorophenyl (1c)], on Candida albicans and Candida krusei biofilm on nanohydroxyapatite substrate, a well-known bioactive ceramic material. To address these goals, both quantitative methods (by cultivable cell numbers) and qualitative evaluation (by scanning electron microscopy) were used. Compounds cytocompatibility towards osteoblast-like cells was also evaluated after 24 h of exposure, through resazurin assay. The three tested compounds displayed a strong inhibitory effect on biofilm development of both Candida species as potent in vitro activity against C. albicans sessile cells. Regarding cytocompatibility, a concentration-dependent effect was observed. Together, these findings indicated that the potent activity of imidazole derivatives on Candida spp. biofilms on nanohydroxyapatite substrate, in particular compound 1c, is worth further investigating. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01T00:00:00Z 2019-01-01T00:00:00Z 2029-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10284/8228 |
url |
http://hdl.handle.net/10284/8228 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0301-486X 10.1007/s11046-019-00400-4 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Verlag |
publisher.none.fl_str_mv |
Springer Verlag |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130316874448896 |