Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regeneration

Detalhes bibliográficos
Autor(a) principal: Serôdio, Ricardo
Data de Publicação: 2019
Outros Autores: Schickert, Sónia L., Costa-Pinto, Ana R., Dias, Juliana R., Granja, Pedro L., Yang, Fang, Oliveira, Ana L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/8563
Resumo: The authors acknowledge Portuguese National Funds from FCT - Fundação para a Ciência e a Tecnologia through project UID/Multi/50016/2013; Program FCT Investigator to A.L. Oliveira (IF/00411/2013), project SERICAMED (IF/00411/2013/CP1167), and through “Biotherapies-Bioengineered Therapies for Infectious Diseases and Tissue Regeneration”, funded by Norte2020. Project “IBEROS” (0245_IBEROS_1_E), funded by Fundo Europeu de Desenvolvimento Regional in the frame of Programa Interreg V A Espanha - Portugal (POCTEP) 2014–2020.
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spelling Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regenerationSilk fibroinPoly(ethylene oxide) membranePeriodontal regenerationElectrospinningUltrasound sonicationThe authors acknowledge Portuguese National Funds from FCT - Fundação para a Ciência e a Tecnologia through project UID/Multi/50016/2013; Program FCT Investigator to A.L. Oliveira (IF/00411/2013), project SERICAMED (IF/00411/2013/CP1167), and through “Biotherapies-Bioengineered Therapies for Infectious Diseases and Tissue Regeneration”, funded by Norte2020. Project “IBEROS” (0245_IBEROS_1_E), funded by Fundo Europeu de Desenvolvimento Regional in the frame of Programa Interreg V A Espanha - Portugal (POCTEP) 2014–2020.In this study, silk fibroin (SF)/poly(ethylene oxide) (PEO) membranes were designed and fabricated by combining ultrasound sonication prior to electrospinning (0 to 20 min) as a strategy to physically control the rheological properties of solutions (10 to 30% w/v PEO) and to improve the spinnability of the system. PEO has proved to be essential as a co-spinning agent to assure good membrane reproducibility and enough flexibility for clinical manipulation. The rheological tests indicated that sonication greatly increased the viscosity of SF/PEO solutions and further enhanced the quality of the produced electrospun fibers with consequent improved mechanical properties in dry and wet conditions. By tuning the viscosity of the solutions using a simple sonication step prior to electrospinning, it was possible to induce water stability in the as-electrospun matrix, as demonstrated by infra-red spectroscopy. This reduced complexity in the process since it was not necessary to concentrate silk prior to electrospinning while avoiding the use of toxic solvents to perform a post-processing stabilization treatment which usually causes dimensional changes to the SF materials. Sonication pre-treatment allowed for minimizing the amount of synthetic polymer used to achieve the desirable mechanical properties (with the modulus ranging between 90 and 170 MPa), while avoiding a further water stabilization treatment. It also had a positive impact in the in vitro cell behavior of human primary periodontal ligament cells (hPDLs), resulting in a marked increase in cell proliferation. The present developed work constitutes a step forward towards simplicity and a better fabrication control of viable electrospun SFbased membranes for periodontal regeneration.Elsevier - ScienceDirectIC-OnlineSerôdio, RicardoSchickert, Sónia L.Costa-Pinto, Ana R.Dias, Juliana R.Granja, Pedro L.Yang, FangOliveira, Ana L.2023-06-05T13:46:45Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/8563engRicardo Serôdio, Sónia L. Schickert, Ana R. Costa-Pinto, Juliana R. Dias, Pedro L. Granja, Fang Yang, Ana L. Oliveira, Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regeneration, Materials Science and Engineering: C, Volume 98, 2019, Pages 969-981, ISSN 0928-4931, https://doi.org/10.1016/j.msec.2019.01.055.0928-4931https://doi.org/10.1016/j.msec.2019.01.05530813104info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:57:35Zoai:iconline.ipleiria.pt:10400.8/8563Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:51:14.920905Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regeneration
title Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regeneration
spellingShingle Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regeneration
Serôdio, Ricardo
Silk fibroin
Poly(ethylene oxide) membrane
Periodontal regeneration
Electrospinning
Ultrasound sonication
title_short Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regeneration
title_full Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regeneration
title_fullStr Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regeneration
title_full_unstemmed Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regeneration
title_sort Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regeneration
author Serôdio, Ricardo
author_facet Serôdio, Ricardo
Schickert, Sónia L.
Costa-Pinto, Ana R.
Dias, Juliana R.
Granja, Pedro L.
Yang, Fang
Oliveira, Ana L.
author_role author
author2 Schickert, Sónia L.
Costa-Pinto, Ana R.
Dias, Juliana R.
Granja, Pedro L.
Yang, Fang
Oliveira, Ana L.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Serôdio, Ricardo
Schickert, Sónia L.
Costa-Pinto, Ana R.
Dias, Juliana R.
Granja, Pedro L.
Yang, Fang
Oliveira, Ana L.
dc.subject.por.fl_str_mv Silk fibroin
Poly(ethylene oxide) membrane
Periodontal regeneration
Electrospinning
Ultrasound sonication
topic Silk fibroin
Poly(ethylene oxide) membrane
Periodontal regeneration
Electrospinning
Ultrasound sonication
description The authors acknowledge Portuguese National Funds from FCT - Fundação para a Ciência e a Tecnologia through project UID/Multi/50016/2013; Program FCT Investigator to A.L. Oliveira (IF/00411/2013), project SERICAMED (IF/00411/2013/CP1167), and through “Biotherapies-Bioengineered Therapies for Infectious Diseases and Tissue Regeneration”, funded by Norte2020. Project “IBEROS” (0245_IBEROS_1_E), funded by Fundo Europeu de Desenvolvimento Regional in the frame of Programa Interreg V A Espanha - Portugal (POCTEP) 2014–2020.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
2023-06-05T13:46:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/8563
url http://hdl.handle.net/10400.8/8563
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ricardo Serôdio, Sónia L. Schickert, Ana R. Costa-Pinto, Juliana R. Dias, Pedro L. Granja, Fang Yang, Ana L. Oliveira, Ultrasound sonication prior to electrospinning tailors silk fibroin/PEO membranes for periodontal regeneration, Materials Science and Engineering: C, Volume 98, 2019, Pages 969-981, ISSN 0928-4931, https://doi.org/10.1016/j.msec.2019.01.055.
0928-4931
https://doi.org/10.1016/j.msec.2019.01.055
30813104
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier - ScienceDirect
publisher.none.fl_str_mv Elsevier - ScienceDirect
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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