Age-Related Changes of the Synucleins Profile in the Mouse Retina
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/149215 |
Resumo: | Funding Information: This research was funded by Fundação para a Ciência e a Tecnologia, grant number PTDC/MED-PAT/29656/2017. L.S. was funded by FCT PhD studentship (SFRH/BD/144743). Additionally, it was supported by iNOVA4Health—UIDB/04462/2020 and UIDP/04462/2020 and by the Associated Laboratory LS4FUTURE (LA/P/0087/2020), two programs financially supported by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior. This work was developed with the support from the research infrastructure Congento, co-financed by Lisboa Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and Fundação para a Ciência e Tecnologia (Portugal) under the project LISBOA-01-0145-FEDER-022170. Publisher Copyright: © 2023 by the authors. |
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Age-Related Changes of the Synucleins Profile in the Mouse RetinaagingdegenerationretinasynucleinBiochemistryMolecular BiologyFunding Information: This research was funded by Fundação para a Ciência e a Tecnologia, grant number PTDC/MED-PAT/29656/2017. L.S. was funded by FCT PhD studentship (SFRH/BD/144743). Additionally, it was supported by iNOVA4Health—UIDB/04462/2020 and UIDP/04462/2020 and by the Associated Laboratory LS4FUTURE (LA/P/0087/2020), two programs financially supported by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior. This work was developed with the support from the research infrastructure Congento, co-financed by Lisboa Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and Fundação para a Ciência e Tecnologia (Portugal) under the project LISBOA-01-0145-FEDER-022170. Publisher Copyright: © 2023 by the authors.Alpha-synuclein (aSyn) plays a central role in Parkinson’s disease (PD) and has been extensively studied in the brain. This protein is part of the synuclein family, which is also composed of beta-synuclein (bSyn) and gamma-synuclein (gSyn). In addition to its neurotoxic role, synucleins have important functions in the nervous system, modulating synaptic transmission. Synucleins are expressed in the retina, but they have been poorly characterized. However, there is evidence that they are important for visual function and that they can play a role in retinal degeneration. This study aimed to profile synucleins in the retina of naturally aged mice and to correlate their patterns with specific retinal cells. With aging, we observed a decrease in the thickness of specific retinal layers, accompanied by an increase in glial reactivity. Moreover, the aSyn levels decreased, whereas bSyn increased with aging. The colocalization of both proteins was decreased in the inner plexiform layer (IPL) of the aged retina. gSyn presented an age-related decrease at the inner nuclear layer but was not significantly changed in the ganglion cell layer. The synaptic marker synaptophysin was shown to be preferentially colocalized with aSyn in the IPL with aging. At the same time, aSyn was found to exist at the presynaptic endings of bipolar cells and was affected by aging. Overall, this study suggests that physiological aging can be responsible for changes in the retinal tissue, implicating functional alterations that could affect synuclein family function.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)iNOVA4Health - pólo NMSRUNDias, Sarah Batistade Lemos, LuísaSousa, LuísBitoque, Diogo B.Silva, Gabriela AraújoSeabra, Miguel C.Tenreiro, Sandra2023-02-14T22:21:31Z2023-012023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/149215eng2218-273XPURE: 52342006https://doi.org/10.3390/biom13010180info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:31:01Zoai:run.unl.pt:10362/149215Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:53:39.675827Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Age-Related Changes of the Synucleins Profile in the Mouse Retina |
| title |
Age-Related Changes of the Synucleins Profile in the Mouse Retina |
| spellingShingle |
Age-Related Changes of the Synucleins Profile in the Mouse Retina Dias, Sarah Batista aging degeneration retina synuclein Biochemistry Molecular Biology |
| title_short |
Age-Related Changes of the Synucleins Profile in the Mouse Retina |
| title_full |
Age-Related Changes of the Synucleins Profile in the Mouse Retina |
| title_fullStr |
Age-Related Changes of the Synucleins Profile in the Mouse Retina |
| title_full_unstemmed |
Age-Related Changes of the Synucleins Profile in the Mouse Retina |
| title_sort |
Age-Related Changes of the Synucleins Profile in the Mouse Retina |
| author |
Dias, Sarah Batista |
| author_facet |
Dias, Sarah Batista de Lemos, Luísa Sousa, Luís Bitoque, Diogo B. Silva, Gabriela Araújo Seabra, Miguel C. Tenreiro, Sandra |
| author_role |
author |
| author2 |
de Lemos, Luísa Sousa, Luís Bitoque, Diogo B. Silva, Gabriela Araújo Seabra, Miguel C. Tenreiro, Sandra |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) iNOVA4Health - pólo NMS RUN |
| dc.contributor.author.fl_str_mv |
Dias, Sarah Batista de Lemos, Luísa Sousa, Luís Bitoque, Diogo B. Silva, Gabriela Araújo Seabra, Miguel C. Tenreiro, Sandra |
| dc.subject.por.fl_str_mv |
aging degeneration retina synuclein Biochemistry Molecular Biology |
| topic |
aging degeneration retina synuclein Biochemistry Molecular Biology |
| description |
Funding Information: This research was funded by Fundação para a Ciência e a Tecnologia, grant number PTDC/MED-PAT/29656/2017. L.S. was funded by FCT PhD studentship (SFRH/BD/144743). Additionally, it was supported by iNOVA4Health—UIDB/04462/2020 and UIDP/04462/2020 and by the Associated Laboratory LS4FUTURE (LA/P/0087/2020), two programs financially supported by Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior. This work was developed with the support from the research infrastructure Congento, co-financed by Lisboa Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and Fundação para a Ciência e Tecnologia (Portugal) under the project LISBOA-01-0145-FEDER-022170. Publisher Copyright: © 2023 by the authors. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-02-14T22:21:31Z 2023-01 2023-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/149215 |
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http://hdl.handle.net/10362/149215 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2218-273X PURE: 52342006 https://doi.org/10.3390/biom13010180 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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