K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy

Detalhes bibliográficos
Autor(a) principal: Ferreira, Joao Vasco
Data de Publicação: 2015
Outros Autores: Soares, Ana Rosa, Ramalho, Jose, Pereira, Paulo, Girao, Henrique
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1038/srep10210
Resumo: We thank Dr. Ana Maria Cuervo for providing the shRNA against Lamp2a and Dr. Cam Patterson for the wild-type and mutant STUB1 constructs. This work was supported by grants from the Portuguese Foundation for Science and Technology (FCT), PTDC/SAU-ORG/113542/2009, PTDC/SAU-ORG/118694/2010, PEST-C/SAU/UI3282/2011-COMPETE and UID/NEU/04539/2013 from FCT.
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spelling K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated AutophagyINDUCIBLE FACTOR-1-ALPHA HIF-1-ALPHAPROTEASOMAL DEGRADATIONTUMOR ANGIOGENESISFACTOR 1-ALPHANITRIC-OXIDELIGASE CHIPRAT-LIVERHYPOXIAPROTEINPATHWAYGeneralWe thank Dr. Ana Maria Cuervo for providing the shRNA against Lamp2a and Dr. Cam Patterson for the wild-type and mutant STUB1 constructs. This work was supported by grants from the Portuguese Foundation for Science and Technology (FCT), PTDC/SAU-ORG/113542/2009, PTDC/SAU-ORG/118694/2010, PEST-C/SAU/UI3282/2011-COMPETE and UID/NEU/04539/2013 from FCT.Chaperone-Mediated Autophagy is a selective form of autophagy. Recently, the degradation of a newly identified CMA substrate, the HIF1A transcription factor, was found to be regulated by the ubiquitin ligase STUB1. In this study we show, for the first time, that K63 ubiquitination is necessary for CMA degradation of HIF1A in vitro and in vivo. Additionally, STUB1 mediates K63 linked ubiquitination of HIF1A. Our findings add a new regulatory step and increase the specificity of the molecular mechanism involved in CMA degradation of HIF1A, expanding the role of ubiquitination to yet another biological process, since the same mechanism might be applicable to other CMA substrates.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNFerreira, Joao VascoSoares, Ana RosaRamalho, JosePereira, PauloGirao, Henrique2017-08-02T22:04:59Z2015-05-112015-05-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1038/srep10210eng2045-2322PURE: 2986923http://www.scopus.com/inward/record.url?scp=84929224667&partnerID=8YFLogxKhttps://doi.org/10.1038/srep10210info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:09:56Zoai:run.unl.pt:10362/22425Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:27:17.503502Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy
title K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy
spellingShingle K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy
Ferreira, Joao Vasco
INDUCIBLE FACTOR-1-ALPHA HIF-1-ALPHA
PROTEASOMAL DEGRADATION
TUMOR ANGIOGENESIS
FACTOR 1-ALPHA
NITRIC-OXIDE
LIGASE CHIP
RAT-LIVER
HYPOXIA
PROTEIN
PATHWAY
General
title_short K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy
title_full K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy
title_fullStr K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy
title_full_unstemmed K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy
title_sort K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy
author Ferreira, Joao Vasco
author_facet Ferreira, Joao Vasco
Soares, Ana Rosa
Ramalho, Jose
Pereira, Paulo
Girao, Henrique
author_role author
author2 Soares, Ana Rosa
Ramalho, Jose
Pereira, Paulo
Girao, Henrique
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Ferreira, Joao Vasco
Soares, Ana Rosa
Ramalho, Jose
Pereira, Paulo
Girao, Henrique
dc.subject.por.fl_str_mv INDUCIBLE FACTOR-1-ALPHA HIF-1-ALPHA
PROTEASOMAL DEGRADATION
TUMOR ANGIOGENESIS
FACTOR 1-ALPHA
NITRIC-OXIDE
LIGASE CHIP
RAT-LIVER
HYPOXIA
PROTEIN
PATHWAY
General
topic INDUCIBLE FACTOR-1-ALPHA HIF-1-ALPHA
PROTEASOMAL DEGRADATION
TUMOR ANGIOGENESIS
FACTOR 1-ALPHA
NITRIC-OXIDE
LIGASE CHIP
RAT-LIVER
HYPOXIA
PROTEIN
PATHWAY
General
description We thank Dr. Ana Maria Cuervo for providing the shRNA against Lamp2a and Dr. Cam Patterson for the wild-type and mutant STUB1 constructs. This work was supported by grants from the Portuguese Foundation for Science and Technology (FCT), PTDC/SAU-ORG/113542/2009, PTDC/SAU-ORG/118694/2010, PEST-C/SAU/UI3282/2011-COMPETE and UID/NEU/04539/2013 from FCT.
publishDate 2015
dc.date.none.fl_str_mv 2015-05-11
2015-05-11T00:00:00Z
2017-08-02T22:04:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1038/srep10210
url https://doi.org/10.1038/srep10210
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
PURE: 2986923
http://www.scopus.com/inward/record.url?scp=84929224667&partnerID=8YFLogxK
https://doi.org/10.1038/srep10210
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137901644087296

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