Strand-specific miR-28-5p and miR-28-3p have distinct effects in colorectal cancer cells

Detalhes bibliográficos
Autor(a) principal: Almeida, Maria L.
Data de Publicação: 2012
Outros Autores: Nicoloso, Milena S., Zeng, Lizhi, Ivan, Cristina, Spizzo, Riccardo, Gafà, Roberta, XIiao, LIianchun, Zhang, Xinna, Vanninii, Ivan, Fanini, Francesca, Fabbri, Muller, Lanza, GIiovanni, Reis, R. M., Zweidler-Mckay, Patrick, Calin, George A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/23926
Resumo: The authors thank Sue Moreau from the Department of Scientific Publications at The University of Texas MD Anderson Cancer Center for English language editing of the manuscript. Author contributions: Study concept and design: M.I.A., P.A.Z, G.A.C. Acquisition of data: M.I.A., L.Z., X.Z. Drafting of the manuscript: M.I.A., M.N., R.S., M.F., R.M.R., P.A.Z, G.A.C. Analysis and interpretation of data: M.I.A., M.N., R.S., R.M., P.A.Z, G.A.C. Critical revision of the manuscript for important intellectual content: M.I.A., M.N., R.S., M.F., R.M.R., P.A.Z, G.A.C. Statistical analysis: M.I.A., C.I., L.X. Obtained funding: G.A.C. Administrative, technical, or material support: R.G., I.V., F.F., M.F., G.L. Study supervision: G.A.C. Drs Nicoloso and Spizzo are currently at the Division of Experimental Oncology, CRO, National Cancer Institute, Aviano, Italy
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spelling Strand-specific miR-28-5p and miR-28-3p have distinct effects in colorectal cancer cellsTranscript regulationGeneRNA ProcessingScience & TechnologyThe authors thank Sue Moreau from the Department of Scientific Publications at The University of Texas MD Anderson Cancer Center for English language editing of the manuscript. Author contributions: Study concept and design: M.I.A., P.A.Z, G.A.C. Acquisition of data: M.I.A., L.Z., X.Z. Drafting of the manuscript: M.I.A., M.N., R.S., M.F., R.M.R., P.A.Z, G.A.C. Analysis and interpretation of data: M.I.A., M.N., R.S., R.M., P.A.Z, G.A.C. Critical revision of the manuscript for important intellectual content: M.I.A., M.N., R.S., M.F., R.M.R., P.A.Z, G.A.C. Statistical analysis: M.I.A., C.I., L.X. Obtained funding: G.A.C. Administrative, technical, or material support: R.G., I.V., F.F., M.F., G.L. Study supervision: G.A.C. Drs Nicoloso and Spizzo are currently at the Division of Experimental Oncology, CRO, National Cancer Institute, Aviano, ItalyBackground & Aims MicroRNAs (miRNAs) can promote or inhibit tumor growth and are therefore being developed as targets for cancer therapies. They are diverse not only in the messenger RNAs (mRNA) they target, but in their production; the same hairpin RNA structure can generate mature products from each strand, termed 5p and 3p, that can bind different mRNAs. We analyzed the expression, functions, and mechanisms of miR-28-5p and miR-28-3p in colorectal cancer (CRC) cells. Methods We measured levels of miR-28-5p and miR-28-3p expression in 108 CRC and 49 normal colorectal samples (47 paired) by reverse transcription, quantitative real-time polymerase chain reaction. The roles of miR-28 in CRC development were studied using cultured HCT116, RKO, and SW480 cells and tumor xenograft analyses in immunodeficient mice; their mRNA targets were also investigated. Results miR-28-5p and miR-28-3p were down-regulated in CRC samples compared with normal colon samples. Overexpression of miRNAs in CRC cells had different effects and the miRNAs interacted with different mRNAs: miR-28-5p altered expression of CCND1 and HOXB3, whereas miR-28-3p bound NM23-H1. Overexpression of miR-28-5p reduced CRC cell proliferation, migration, and invasion in vitro, whereas miR-28-3p increased CRC cell migration and invasion in vitro. CRC cells overexpressing miR-28 developed tumors more slowly in mice compared with control cells, but miR-28 promoted tumor metastasis in mice. Conclusion miR-28-5p and miR-28-3p are transcribed from the same RNA hairpin and are down-regulated in CRC cells. Overexpression of each has different effects on CRC cell proliferation and migration. Such information has a direct application for the design of miRNA gene therapy trials.M.I.A. is supported by a PhD fellowship (SFRH/BD/47031/2008) from Fundação para a Ciência e Tecnologia, Portugal. G.A.C. is supported as a fellow by The University of Texas MD Anderson Cancer Center Research Trust, The University of Texas System Regents Research Scholar, and the Chronic Lymphocytic Leukemia Global Research Foundation. Work in Dr Calin’s laboratory is supported in part by grants from the National Institutes of Health (CA135444), the US Department of Defense, the Pancreatic Cancer Action Network (2009 Seena Magowitz AACR Pilot Grant), and the US-European Alliance for the Therapy of Chronic Lymphoid Leukemia. STR DNA fingerprinting was done by the Cancer Center Support grant funded Characterized Cell Line core, NCI # CA16672.WB SaundersUniversidade do MinhoAlmeida, Maria L.Nicoloso, Milena S.Zeng, LizhiIvan, CristinaSpizzo, RiccardoGafà, RobertaXIiao, LIianchunZhang, XinnaVanninii, IvanFanini, FrancescaFabbri, MullerLanza, GIiovanniReis, R. M.Zweidler-Mckay, PatrickCalin, George A.2012-042012-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/23926eng0016-508510.1053/j.gastro.2011.12.04722240480http://dx.doi.org/10.1053%2Fj.gastro.2011.12.047info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:06:39Zoai:repositorium.sdum.uminho.pt:1822/23926Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:57:25.135379Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Strand-specific miR-28-5p and miR-28-3p have distinct effects in colorectal cancer cells
title Strand-specific miR-28-5p and miR-28-3p have distinct effects in colorectal cancer cells
spellingShingle Strand-specific miR-28-5p and miR-28-3p have distinct effects in colorectal cancer cells
Almeida, Maria L.
Transcript regulation
Gene
RNA Processing
Science & Technology
title_short Strand-specific miR-28-5p and miR-28-3p have distinct effects in colorectal cancer cells
title_full Strand-specific miR-28-5p and miR-28-3p have distinct effects in colorectal cancer cells
title_fullStr Strand-specific miR-28-5p and miR-28-3p have distinct effects in colorectal cancer cells
title_full_unstemmed Strand-specific miR-28-5p and miR-28-3p have distinct effects in colorectal cancer cells
title_sort Strand-specific miR-28-5p and miR-28-3p have distinct effects in colorectal cancer cells
author Almeida, Maria L.
author_facet Almeida, Maria L.
Nicoloso, Milena S.
Zeng, Lizhi
Ivan, Cristina
Spizzo, Riccardo
Gafà, Roberta
XIiao, LIianchun
Zhang, Xinna
Vanninii, Ivan
Fanini, Francesca
Fabbri, Muller
Lanza, GIiovanni
Reis, R. M.
Zweidler-Mckay, Patrick
Calin, George A.
author_role author
author2 Nicoloso, Milena S.
Zeng, Lizhi
Ivan, Cristina
Spizzo, Riccardo
Gafà, Roberta
XIiao, LIianchun
Zhang, Xinna
Vanninii, Ivan
Fanini, Francesca
Fabbri, Muller
Lanza, GIiovanni
Reis, R. M.
Zweidler-Mckay, Patrick
Calin, George A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Almeida, Maria L.
Nicoloso, Milena S.
Zeng, Lizhi
Ivan, Cristina
Spizzo, Riccardo
Gafà, Roberta
XIiao, LIianchun
Zhang, Xinna
Vanninii, Ivan
Fanini, Francesca
Fabbri, Muller
Lanza, GIiovanni
Reis, R. M.
Zweidler-Mckay, Patrick
Calin, George A.
dc.subject.por.fl_str_mv Transcript regulation
Gene
RNA Processing
Science & Technology
topic Transcript regulation
Gene
RNA Processing
Science & Technology
description The authors thank Sue Moreau from the Department of Scientific Publications at The University of Texas MD Anderson Cancer Center for English language editing of the manuscript. Author contributions: Study concept and design: M.I.A., P.A.Z, G.A.C. Acquisition of data: M.I.A., L.Z., X.Z. Drafting of the manuscript: M.I.A., M.N., R.S., M.F., R.M.R., P.A.Z, G.A.C. Analysis and interpretation of data: M.I.A., M.N., R.S., R.M., P.A.Z, G.A.C. Critical revision of the manuscript for important intellectual content: M.I.A., M.N., R.S., M.F., R.M.R., P.A.Z, G.A.C. Statistical analysis: M.I.A., C.I., L.X. Obtained funding: G.A.C. Administrative, technical, or material support: R.G., I.V., F.F., M.F., G.L. Study supervision: G.A.C. Drs Nicoloso and Spizzo are currently at the Division of Experimental Oncology, CRO, National Cancer Institute, Aviano, Italy
publishDate 2012
dc.date.none.fl_str_mv 2012-04
2012-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/23926
url http://hdl.handle.net/1822/23926
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0016-5085
10.1053/j.gastro.2011.12.047
22240480
http://dx.doi.org/10.1053%2Fj.gastro.2011.12.047
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv WB Saunders
publisher.none.fl_str_mv WB Saunders
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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