Cyanobacterial allelochemicals but not cyanobacterial cells markedly reduce microbial community diversity
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/120363 |
Resumo: | The freshwater cyanobacterium Phormidium sp. LEGE 05292 produces allelochemicals, including the cyclic depsipeptides portoamides, that influence the growth of heterotrophic bacteria, cyanobacteria, and eukaryotic algae. Using 16S rRNA gene amplicon metagenomics, we show here that, under laboratory conditions, the mixture of metabolites exuded by Phormidium sp. LEGE 05292 markedly reduces the diversity of a natural planktonic microbial community. Exposure of the same community to the portoamides alone resulted in a similar outcome. In both cases, after 16 days, alpha-diversity estimates for the allelochemical-exposed communities were less than half of those for the control communities. Photosynthetic organisms, but also different heterotrophic-bacteria taxa were found to be negatively impacted by the allelochemicals. Intriguingly, when Phormidium sp. LEGE 05292 was co-cultured with the microbial community, the latter remained stable and closer to non-exposed than to allelochemical-exposed communities. Overall, our observations indicate that although under optimal growth conditions Phormidium sp. LEGE 05292 is able to synthesize potent allelochemicals that severely impact different microorganisms, its allelopathic effect is not pronounced when in contact with a complex microbial community. Therefore, under ecologically relevant conditions, the allelopathic behavior of this cyanobacterium may be regulated by nutrient availability or by interactions with the surrounding microbiota. Copyright © 2017 Dias, Antunes, Ribeiro, Azevedo, Vasconcelos and Leão. |
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Cyanobacterial allelochemicals but not cyanobacterial cells markedly reduce microbial community diversityRNA 16SacclimatizationArticlecolumn chromatographycyanobacteriumDNA extractionelectrosprayhigh performance liquid chromatographymetagenomicsmicrobial communitynonhumannutrient availabilitysequence analysisThe freshwater cyanobacterium Phormidium sp. LEGE 05292 produces allelochemicals, including the cyclic depsipeptides portoamides, that influence the growth of heterotrophic bacteria, cyanobacteria, and eukaryotic algae. Using 16S rRNA gene amplicon metagenomics, we show here that, under laboratory conditions, the mixture of metabolites exuded by Phormidium sp. LEGE 05292 markedly reduces the diversity of a natural planktonic microbial community. Exposure of the same community to the portoamides alone resulted in a similar outcome. In both cases, after 16 days, alpha-diversity estimates for the allelochemical-exposed communities were less than half of those for the control communities. Photosynthetic organisms, but also different heterotrophic-bacteria taxa were found to be negatively impacted by the allelochemicals. Intriguingly, when Phormidium sp. LEGE 05292 was co-cultured with the microbial community, the latter remained stable and closer to non-exposed than to allelochemical-exposed communities. Overall, our observations indicate that although under optimal growth conditions Phormidium sp. LEGE 05292 is able to synthesize potent allelochemicals that severely impact different microorganisms, its allelopathic effect is not pronounced when in contact with a complex microbial community. Therefore, under ecologically relevant conditions, the allelopathic behavior of this cyanobacterium may be regulated by nutrient availability or by interactions with the surrounding microbiota. Copyright © 2017 Dias, Antunes, Ribeiro, Azevedo, Vasconcelos and Leão.Frontiers Media20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/120363eng166430210.3389/fmicb.2017.01495Dias F.Antunes J.T.Ribeiro T.Azevedo J.Vasconcelos V.Leão P.N.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-27T07:44:22Zoai:repositorio-aberto.up.pt:10216/120363Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-27T07:44:22Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Cyanobacterial allelochemicals but not cyanobacterial cells markedly reduce microbial community diversity |
title |
Cyanobacterial allelochemicals but not cyanobacterial cells markedly reduce microbial community diversity |
spellingShingle |
Cyanobacterial allelochemicals but not cyanobacterial cells markedly reduce microbial community diversity Dias F. RNA 16S acclimatization Article column chromatography cyanobacterium DNA extraction electrospray high performance liquid chromatography metagenomics microbial community nonhuman nutrient availability sequence analysis |
title_short |
Cyanobacterial allelochemicals but not cyanobacterial cells markedly reduce microbial community diversity |
title_full |
Cyanobacterial allelochemicals but not cyanobacterial cells markedly reduce microbial community diversity |
title_fullStr |
Cyanobacterial allelochemicals but not cyanobacterial cells markedly reduce microbial community diversity |
title_full_unstemmed |
Cyanobacterial allelochemicals but not cyanobacterial cells markedly reduce microbial community diversity |
title_sort |
Cyanobacterial allelochemicals but not cyanobacterial cells markedly reduce microbial community diversity |
author |
Dias F. |
author_facet |
Dias F. Antunes J.T. Ribeiro T. Azevedo J. Vasconcelos V. Leão P.N. |
author_role |
author |
author2 |
Antunes J.T. Ribeiro T. Azevedo J. Vasconcelos V. Leão P.N. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Dias F. Antunes J.T. Ribeiro T. Azevedo J. Vasconcelos V. Leão P.N. |
dc.subject.por.fl_str_mv |
RNA 16S acclimatization Article column chromatography cyanobacterium DNA extraction electrospray high performance liquid chromatography metagenomics microbial community nonhuman nutrient availability sequence analysis |
topic |
RNA 16S acclimatization Article column chromatography cyanobacterium DNA extraction electrospray high performance liquid chromatography metagenomics microbial community nonhuman nutrient availability sequence analysis |
description |
The freshwater cyanobacterium Phormidium sp. LEGE 05292 produces allelochemicals, including the cyclic depsipeptides portoamides, that influence the growth of heterotrophic bacteria, cyanobacteria, and eukaryotic algae. Using 16S rRNA gene amplicon metagenomics, we show here that, under laboratory conditions, the mixture of metabolites exuded by Phormidium sp. LEGE 05292 markedly reduces the diversity of a natural planktonic microbial community. Exposure of the same community to the portoamides alone resulted in a similar outcome. In both cases, after 16 days, alpha-diversity estimates for the allelochemical-exposed communities were less than half of those for the control communities. Photosynthetic organisms, but also different heterotrophic-bacteria taxa were found to be negatively impacted by the allelochemicals. Intriguingly, when Phormidium sp. LEGE 05292 was co-cultured with the microbial community, the latter remained stable and closer to non-exposed than to allelochemical-exposed communities. Overall, our observations indicate that although under optimal growth conditions Phormidium sp. LEGE 05292 is able to synthesize potent allelochemicals that severely impact different microorganisms, its allelopathic effect is not pronounced when in contact with a complex microbial community. Therefore, under ecologically relevant conditions, the allelopathic behavior of this cyanobacterium may be regulated by nutrient availability or by interactions with the surrounding microbiota. Copyright © 2017 Dias, Antunes, Ribeiro, Azevedo, Vasconcelos and Leão. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/120363 |
url |
https://hdl.handle.net/10216/120363 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1664302 10.3389/fmicb.2017.01495 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
_version_ |
1817547666569035776 |