Microbiota Modulation With Synbiotic Decreases Liver Fibrosis in a High Fat Choline Deficient Diet Mice Model of Non-Alcoholic Steatohepatitis (NASH)

Detalhes bibliográficos
Autor(a) principal: Cortez-Pinto, Helena
Data de Publicação: 2016
Outros Autores: Borralho, Paula, Machado, Jorge, Lopes, Maria T., Gato, Inês V., Santos, António M., Guerreiro, António S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/124600
Resumo: Funding Information: This work is supported by a grant from Fundação para a Ciência e a Tecnologia ( PTDC-SAU-OSM/66323/2006 ). Publisher Copyright: © 2016 Sociedade Portuguesa de Gastrenterologia.
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spelling Microbiota Modulation With Synbiotic Decreases Liver Fibrosis in a High Fat Choline Deficient Diet Mice Model of Non-Alcoholic Steatohepatitis (NASH)MiceMicrobiotaNon-alcoholic Fatty Liver DiseasePrebioticsProbioticsGastroenterologyFunding Information: This work is supported by a grant from Fundação para a Ciência e a Tecnologia ( PTDC-SAU-OSM/66323/2006 ). Publisher Copyright: © 2016 Sociedade Portuguesa de Gastrenterologia.Background: Gut microbiota may play a role in non-alcoholic steatohepatitis (NASH). Previous studies showed that prebiotics and probiotics might halt the progression of steatohepatitis. Aim: To clarify the potential effect of Synbiotic 2000®Forte (Synb) in preventing or ameliorating diet induced steatohepatitis, particularly in fibrosis progression and how this intervention correlates with gut microbiota composition and endotoxinemia. Methods: Twenty-seven C57BL/6 mice were divided into three groups: chow diet (CD, n = 7); high-fat choline deficient diet (HFCD, n = 10) and HFCD diet supplemented with Synbiotic 2000®Forte (four probiotic strains and four prebiotics mixture) (HFCD + Synb, n = 10). At 6 and 18 weeks, blood samples (lipopolysaccharides assay - LPS), cecal feaces (gut microbiota) and liver tissue (histology) were collected for analysis. Results: Both HCFD diet mice developed steatohepatitis with ballooning at 6 and 18 weeks, opposite to CD. Comparison of histological scores in HFCD and HFCD + Synb, at 6 and 18 weeks showed no significant difference regarding steatosis, inflammation, or ballooning. Evaluating fibrosis with Sirius Red, and degree of smooth-muscle cell activation, HFCD mice had significantly more fibrosis; addition of Synb significantly reduced fibrosis at 6 weeks and 18 weeks. Serum endotoxin levels were similarly increased in HFCD and HFCD + Synb at week 6; however at week 18 HFCD + Synb had significantly lower endotoxin levels than HFCD. Gut microbiota of HFCD vs CD, showed no significant differences regarding the phyla Firmicutes and Bacteroidetes, either at 6 or 18 weeks; Proteobacteria increased at 6 week (3.3) and 18 week (7.5), while the addition of Synb resulted in a decrease at week 18 (-3.90). Fusobacteria markedly increase at week 18 (10.0), but less so with the addition of Synb (5.2). Conclusion: Synbiotic 2000®Forte is able to modulate the mouse gut microbiota reducing the degree of fibrosis while simultaneously decreasing endotoxemia.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNCortez-Pinto, HelenaBorralho, PaulaMachado, JorgeLopes, Maria T.Gato, Inês V.Santos, António M.Guerreiro, António S.2021-09-15T00:31:43Z2016-05-012016-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/10362/124600eng2341-4545PURE: 33721912https://doi.org/10.1016/j.jpge.2016.01.004info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:05:56Zoai:run.unl.pt:10362/124600Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:45:32.205508Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microbiota Modulation With Synbiotic Decreases Liver Fibrosis in a High Fat Choline Deficient Diet Mice Model of Non-Alcoholic Steatohepatitis (NASH)
title Microbiota Modulation With Synbiotic Decreases Liver Fibrosis in a High Fat Choline Deficient Diet Mice Model of Non-Alcoholic Steatohepatitis (NASH)
spellingShingle Microbiota Modulation With Synbiotic Decreases Liver Fibrosis in a High Fat Choline Deficient Diet Mice Model of Non-Alcoholic Steatohepatitis (NASH)
Cortez-Pinto, Helena
Mice
Microbiota
Non-alcoholic Fatty Liver Disease
Prebiotics
Probiotics
Gastroenterology
title_short Microbiota Modulation With Synbiotic Decreases Liver Fibrosis in a High Fat Choline Deficient Diet Mice Model of Non-Alcoholic Steatohepatitis (NASH)
title_full Microbiota Modulation With Synbiotic Decreases Liver Fibrosis in a High Fat Choline Deficient Diet Mice Model of Non-Alcoholic Steatohepatitis (NASH)
title_fullStr Microbiota Modulation With Synbiotic Decreases Liver Fibrosis in a High Fat Choline Deficient Diet Mice Model of Non-Alcoholic Steatohepatitis (NASH)
title_full_unstemmed Microbiota Modulation With Synbiotic Decreases Liver Fibrosis in a High Fat Choline Deficient Diet Mice Model of Non-Alcoholic Steatohepatitis (NASH)
title_sort Microbiota Modulation With Synbiotic Decreases Liver Fibrosis in a High Fat Choline Deficient Diet Mice Model of Non-Alcoholic Steatohepatitis (NASH)
author Cortez-Pinto, Helena
author_facet Cortez-Pinto, Helena
Borralho, Paula
Machado, Jorge
Lopes, Maria T.
Gato, Inês V.
Santos, António M.
Guerreiro, António S.
author_role author
author2 Borralho, Paula
Machado, Jorge
Lopes, Maria T.
Gato, Inês V.
Santos, António M.
Guerreiro, António S.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Cortez-Pinto, Helena
Borralho, Paula
Machado, Jorge
Lopes, Maria T.
Gato, Inês V.
Santos, António M.
Guerreiro, António S.
dc.subject.por.fl_str_mv Mice
Microbiota
Non-alcoholic Fatty Liver Disease
Prebiotics
Probiotics
Gastroenterology
topic Mice
Microbiota
Non-alcoholic Fatty Liver Disease
Prebiotics
Probiotics
Gastroenterology
description Funding Information: This work is supported by a grant from Fundação para a Ciência e a Tecnologia ( PTDC-SAU-OSM/66323/2006 ). Publisher Copyright: © 2016 Sociedade Portuguesa de Gastrenterologia.
publishDate 2016
dc.date.none.fl_str_mv 2016-05-01
2016-05-01T00:00:00Z
2021-09-15T00:31:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/124600
url http://hdl.handle.net/10362/124600
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2341-4545
PURE: 33721912
https://doi.org/10.1016/j.jpge.2016.01.004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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