Added value of lymphocyte subpopulations in the classification of Sjögren's syndrome

Detalhes bibliográficos
Autor(a) principal: Barcelos, Filipe
Data de Publicação: 2023
Outros Autores: Brás-Geraldes, Carlos, Martins, Catarina, Papoila, Ana Luísa, Monteiro, Ricardo, Cardigos, Joana, Madeira, Nathalie, Alves, Nuno, Vaz-Patto, José, Cunha-Branco, Jaime, Borrego, Luís Miguel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/153050
Resumo: Funding Information: The first author gratefully acknowledges Academia Cuf/José de Mello Saúde and Sociedade Portuguesa de Reumatologia for their financial support. The authors gratefully acknowledge the laboratory staff that assisted in the flow cytometry analysis. Publisher Copyright: © 2023, The Author(s).
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spelling Added value of lymphocyte subpopulations in the classification of Sjögren's syndromeGeneralFunding Information: The first author gratefully acknowledges Academia Cuf/José de Mello Saúde and Sociedade Portuguesa de Reumatologia for their financial support. The authors gratefully acknowledge the laboratory staff that assisted in the flow cytometry analysis. Publisher Copyright: © 2023, The Author(s).Sjögren's Syndrome (SjS) is a chronic systemic immune-mediated inflammatory disease characterized by lymphocytic infiltration and consequent lesion of exocrine glands. SjS diagnosis and classification remains a challenge, especially at SjS onset, when patients may have milder phenotypes of the disease or uncommon presentations. New biomarkers are needed for the classification of SjS, thus, we aimed to evaluate the added-value of lymphocyte subpopulations in discriminating SjS and non-Sjögren Sicca patients. Lymphocyte subsets from 62 SjS and 63 Sicca patients were characterized by flow cytometry. The 2002 AECG and the 2016 ACR/EULAR SjS classification criteria were compared with clinical diagnosis. The added discriminative ability of joining lymphocytic populations to classification criteria was assessed by the area under the Receiver-Operating-Characteristic Curve (AUC). Considering clinical diagnosis as the gold-standard, we obtained an AUC = 0.952 (95% CI: 0.916–0.989) for AECG and an AUC = 0.921 (95% CI: 0.875–0.966) for ACR/EULAR criteria. Adding Tfh and Bm1 subsets to AECG criteria, performance increased, attaining an AUC = 0.985 (95% CI: 0.968–1.000) (p = 0.021). Th1/Breg-like CD24hiCD27+ and switched-memory B-cells maximized the AUC of ACR/EULAR criteria to 0.953 (95% CI: 0.916–0.990) (p = 0.043). Our exploratory study supports the potential use of lymphocyte subpopulations, such as unswitched memory B cells, to improve the performance of classification criteria, since their discriminative ability increases when specific subsets are added to the criteria.Comprehensive Health Research Centre (CHRC) - pólo NMSNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNBarcelos, FilipeBrás-Geraldes, CarlosMartins, CatarinaPapoila, Ana LuísaMonteiro, RicardoCardigos, JoanaMadeira, NathalieAlves, NunoVaz-Patto, JoséCunha-Branco, JaimeBorrego, Luís Miguel2023-05-22T22:18:18Z2023-04-272023-04-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/153050eng2045-2322PURE: 60955540https://doi.org/10.1038/s41598-023-31782-7info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:35:35Zoai:run.unl.pt:10362/153050Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:55:09.092069Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Added value of lymphocyte subpopulations in the classification of Sjögren's syndrome
title Added value of lymphocyte subpopulations in the classification of Sjögren's syndrome
spellingShingle Added value of lymphocyte subpopulations in the classification of Sjögren's syndrome
Barcelos, Filipe
General
title_short Added value of lymphocyte subpopulations in the classification of Sjögren's syndrome
title_full Added value of lymphocyte subpopulations in the classification of Sjögren's syndrome
title_fullStr Added value of lymphocyte subpopulations in the classification of Sjögren's syndrome
title_full_unstemmed Added value of lymphocyte subpopulations in the classification of Sjögren's syndrome
title_sort Added value of lymphocyte subpopulations in the classification of Sjögren's syndrome
author Barcelos, Filipe
author_facet Barcelos, Filipe
Brás-Geraldes, Carlos
Martins, Catarina
Papoila, Ana Luísa
Monteiro, Ricardo
Cardigos, Joana
Madeira, Nathalie
Alves, Nuno
Vaz-Patto, José
Cunha-Branco, Jaime
Borrego, Luís Miguel
author_role author
author2 Brás-Geraldes, Carlos
Martins, Catarina
Papoila, Ana Luísa
Monteiro, Ricardo
Cardigos, Joana
Madeira, Nathalie
Alves, Nuno
Vaz-Patto, José
Cunha-Branco, Jaime
Borrego, Luís Miguel
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Comprehensive Health Research Centre (CHRC) - pólo NMS
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Barcelos, Filipe
Brás-Geraldes, Carlos
Martins, Catarina
Papoila, Ana Luísa
Monteiro, Ricardo
Cardigos, Joana
Madeira, Nathalie
Alves, Nuno
Vaz-Patto, José
Cunha-Branco, Jaime
Borrego, Luís Miguel
dc.subject.por.fl_str_mv General
topic General
description Funding Information: The first author gratefully acknowledges Academia Cuf/José de Mello Saúde and Sociedade Portuguesa de Reumatologia for their financial support. The authors gratefully acknowledge the laboratory staff that assisted in the flow cytometry analysis. Publisher Copyright: © 2023, The Author(s).
publishDate 2023
dc.date.none.fl_str_mv 2023-05-22T22:18:18Z
2023-04-27
2023-04-27T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/153050
url http://hdl.handle.net/10362/153050
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
PURE: 60955540
https://doi.org/10.1038/s41598-023-31782-7
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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