Implementation of membrane processes for improvement of the detection of food contaminants

Detalhes bibliográficos
Autor(a) principal: Deb, Tanmoy Kanti
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/16425
Resumo: The EM3E Master is an Education Programme supported by the European Commission, the European Membrane Society (EMS), the European Membrane House (EMH), and a large international network of industrial companies, research centres and universities (http://www.em3e.eu)
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spelling Implementation of membrane processes for improvement of the detection of food contaminantsMembrane processesMilkFood contaminantMembrane rejectionCell isolationDomínio/Área Científica::Engenharia e Tecnologia::Engenharia QuímicaThe EM3E Master is an Education Programme supported by the European Commission, the European Membrane Society (EMS), the European Membrane House (EMH), and a large international network of industrial companies, research centres and universities (http://www.em3e.eu)Food borne pathogen is a topmost important concern of present era due to deteriorating the food quality, contributing significant loss in the health aspect, global nutritional demand and economy. A rapid, fast and effective analytic system of pathogen identification and enumeration is the vital most important tool towards minimization of both the grasp and effect of pathogen contamination whereas the performance and accuracy of almost all of presently developed respective sensory tools are localized on the molecular recognition and catalytic processes of the sensor that work for the detection and enumeration. The presence of other biological molecules except the targets imparts significant unwanted ligand interaction which deteriorates its true binding efficiency of the specific ligand with exact orientation and frequency; and thus requires the pre-treatments of the sample to minimize such interference. Keeping this on mind, in this work, a membrane process has developed for the purification of the targets (the cells) by removal of the other unwanted interfering species from the Listeria innocua loaded food (milk) sample followed by concentration of the sample. Both the type of bacteria and host food were selected due to high demand. The selective concentration of the cells and volume reduction of target cells from the milk sample was accomplished by diafiltration (DF) and tangential flow filtration (TFF) with microfiltration (MF) membrane. The effect of cell loading on the cell purification and concentration by filtration performance of milk was analyzed. Comparative analysis between TFF and DF was done on this aspect and a combined protocol has developed. The analysis of operational parameters (e.g. TMP, CFV, permeate flux behaviour, system configuration, etc.) on the chemical composition of permeate and retentate was studied. A mass-balance based model was developed and used to predict the trend of concentrations of the species along the progress of DF process. The organic load removal from the system was found to be positively correlated with the TMP and CFV involved while the cell recovery was found efficient in an optimum TMP range. The system was found efficient with fastness, effective cell purifying and concentrating capability, high recovery of membrane and easiness to operate with more than 99.7% of viable cell isolation for further use in bio-sensing tool.Moreira, CarlaRUNDeb, Tanmoy Kanti2016-02-10T10:41:59Z2015-072016-022015-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/16425enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:53:21Zoai:run.unl.pt:10362/16425Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:23:14.087295Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Implementation of membrane processes for improvement of the detection of food contaminants
title Implementation of membrane processes for improvement of the detection of food contaminants
spellingShingle Implementation of membrane processes for improvement of the detection of food contaminants
Deb, Tanmoy Kanti
Membrane processes
Milk
Food contaminant
Membrane rejection
Cell isolation
Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química
title_short Implementation of membrane processes for improvement of the detection of food contaminants
title_full Implementation of membrane processes for improvement of the detection of food contaminants
title_fullStr Implementation of membrane processes for improvement of the detection of food contaminants
title_full_unstemmed Implementation of membrane processes for improvement of the detection of food contaminants
title_sort Implementation of membrane processes for improvement of the detection of food contaminants
author Deb, Tanmoy Kanti
author_facet Deb, Tanmoy Kanti
author_role author
dc.contributor.none.fl_str_mv Moreira, Carla
RUN
dc.contributor.author.fl_str_mv Deb, Tanmoy Kanti
dc.subject.por.fl_str_mv Membrane processes
Milk
Food contaminant
Membrane rejection
Cell isolation
Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química
topic Membrane processes
Milk
Food contaminant
Membrane rejection
Cell isolation
Domínio/Área Científica::Engenharia e Tecnologia::Engenharia Química
description The EM3E Master is an Education Programme supported by the European Commission, the European Membrane Society (EMS), the European Membrane House (EMH), and a large international network of industrial companies, research centres and universities (http://www.em3e.eu)
publishDate 2015
dc.date.none.fl_str_mv 2015-07
2015-07-01T00:00:00Z
2016-02-10T10:41:59Z
2016-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/16425
url http://hdl.handle.net/10362/16425
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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