The role of I-FABP as a biomarker of intestinal barrier dysfunction driven by gut microbiota changes in obesity

Detalhes bibliográficos
Autor(a) principal: Lau, Eva
Data de Publicação: 2016
Outros Autores: Marques, Claudia, Pestana, Diogo, Santoalha, Mariana, Carvalho, Davide, Freitas, Paula, Calhau, Conceição
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/22378
Resumo: Financial support from Fundacao para Ciencia e Tecnologia (PTDC/AGR-TEC/2227/2012 and SFRH/BD/93073/2013) is gratefully acknowledged.
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spelling The role of I-FABP as a biomarker of intestinal barrier dysfunction driven by gut microbiota changes in obesityGut microbiotaInflammationIntestinal fatty acid binding proteinIntestinal permeabilityObesityNutrition and DieteticsSDG 3 - Good Health and Well-beingFinancial support from Fundacao para Ciencia e Tecnologia (PTDC/AGR-TEC/2227/2012 and SFRH/BD/93073/2013) is gratefully acknowledged.Background: Intestinal fatty-acid binding protein (I-FABP) is expressed in epithelial cells of the mucosal layer of the small intestine tissue. When intestinal mucosal damage occurs, I-FABP is released into the circulation and its plasma concentration increases. In the context of obesity, the gut barrier integrity can be disrupted by dietary fat while intestinal permeability increases. Objective: To investigate whether intestinal fatty acid binding protein (I-FABP) is a suitable plasma marker of intestinal injury and inflammation in obesity. Methods: Twelve male Wistar rats were randomly divided into two groups of six animals each: standard (St) and high-fat (HF) diet fed groups for 12 weeks. Results: HF fed animals developed obesity, insulin resistance and seemed to present increased plasma levels of proinflammatory cytokines (MCP-1 and IL1 beta). The gut microbiota composition of these animals was also altered, with lower number of copies of Bacteroidetes, Prevotella spp. and Lactobacillus spp., in comparison with those from St diet group. Fecal lipopolysaccharide (LPS) concentrations tended to be increased in HF fed animals. Intestinal expression of TLR4 seemed to be also increased in HF fed animals suggesting that HF diet-induced dysbiosis may be behind the systemic inflammation observed. However, in contrast to other intestinal inflammatory diseases, plasma I-FABP levels were decreased in HF fed rats whereas I-FABP expression in jejunum tended to be increased. Conclusions: HF diet-induced obesity is characterized by dysbiosis, insulin resistance and systemic inflammation. In this context, plasmatic I-FABP should not be used as a marker of the intestinal barrier dysfunction and the low-grade chronic inflammatory status.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNLau, EvaMarques, ClaudiaPestana, DiogoSantoalha, MarianaCarvalho, DavideFreitas, PaulaCalhau, Conceição2017-08-01T22:03:02Z2016-04-302016-04-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/22378eng1743-7075PURE: 2350988https://doi.org/10.1186/s12986-016-0089-7info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-06-10T01:43:41Zoai:run.unl.pt:10362/22378Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-06-10T01:43:41Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The role of I-FABP as a biomarker of intestinal barrier dysfunction driven by gut microbiota changes in obesity
title The role of I-FABP as a biomarker of intestinal barrier dysfunction driven by gut microbiota changes in obesity
spellingShingle The role of I-FABP as a biomarker of intestinal barrier dysfunction driven by gut microbiota changes in obesity
Lau, Eva
Gut microbiota
Inflammation
Intestinal fatty acid binding protein
Intestinal permeability
Obesity
Nutrition and Dietetics
SDG 3 - Good Health and Well-being
title_short The role of I-FABP as a biomarker of intestinal barrier dysfunction driven by gut microbiota changes in obesity
title_full The role of I-FABP as a biomarker of intestinal barrier dysfunction driven by gut microbiota changes in obesity
title_fullStr The role of I-FABP as a biomarker of intestinal barrier dysfunction driven by gut microbiota changes in obesity
title_full_unstemmed The role of I-FABP as a biomarker of intestinal barrier dysfunction driven by gut microbiota changes in obesity
title_sort The role of I-FABP as a biomarker of intestinal barrier dysfunction driven by gut microbiota changes in obesity
author Lau, Eva
author_facet Lau, Eva
Marques, Claudia
Pestana, Diogo
Santoalha, Mariana
Carvalho, Davide
Freitas, Paula
Calhau, Conceição
author_role author
author2 Marques, Claudia
Pestana, Diogo
Santoalha, Mariana
Carvalho, Davide
Freitas, Paula
Calhau, Conceição
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Lau, Eva
Marques, Claudia
Pestana, Diogo
Santoalha, Mariana
Carvalho, Davide
Freitas, Paula
Calhau, Conceição
dc.subject.por.fl_str_mv Gut microbiota
Inflammation
Intestinal fatty acid binding protein
Intestinal permeability
Obesity
Nutrition and Dietetics
SDG 3 - Good Health and Well-being
topic Gut microbiota
Inflammation
Intestinal fatty acid binding protein
Intestinal permeability
Obesity
Nutrition and Dietetics
SDG 3 - Good Health and Well-being
description Financial support from Fundacao para Ciencia e Tecnologia (PTDC/AGR-TEC/2227/2012 and SFRH/BD/93073/2013) is gratefully acknowledged.
publishDate 2016
dc.date.none.fl_str_mv 2016-04-30
2016-04-30T00:00:00Z
2017-08-01T22:03:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/22378
url http://hdl.handle.net/10362/22378
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1743-7075
PURE: 2350988
https://doi.org/10.1186/s12986-016-0089-7
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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