Tuning the Biological Activity of Camphorimine Complexes through Metal Selection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/145314 |
Resumo: | the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. |
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Tuning the Biological Activity of Camphorimine Complexes through Metal Selectioncamphoriminecaspase 3caspase 7cisplatincopper complexgold complexmetal complexreactive oxygen metaboliteunclassified drugagar gel electrophoresisapoptosisArticlebiological activityCaenorhabditis eleganscell viabilityclinical articlecytotoxicitydispersityDPPH radical scavenging assayelectrophoretic mobilityelemental analysisfibroblastgenetic transfectionhumanhuman cellIC50LC50limit of detectionlipid peroxidationlipophilicityMTT assayOVCAR-3 cell lineoxidative stressultraviolet spectrophotometryV79 cell lineX rayX ray diffractionSDG 3 - Good Health and Well-beingthe project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB.The cytotoxic activity of four sets of camphorimine complexes based on the Cu(I), Cu(II), Ag(I), and Au(I) metal sites were assessed against the cisplatin-sensitive A2780 and OVCAR3 ovarian cancer cells. The results showed that the gold complexes were ca. one order of magnitude more active than the silver complexes, which in turn were ca. one order of magnitude more active than the copper complexes. An important finding was that the cytotoxic activity of the Ag(I) and Au(I) camphorimine complexes was higher than that of cisplatin. Another relevant aspect was that the camphorimine complexes did not interact significantly with DNA, in contrast with cisplatin. The cytotoxic activity of the camphorimine complexes displayed a direct relationship with the cellular uptake by OVCAR3 cells, as ascertained by PIXE (particle-induced X-ray emission). The levels of ROS (reactive oxygen species) formation exhibited an inverse relationship with the reduction potentials for the complexes with the same metal, as assessed by cyclic voltammetry. In order to gain insight into the toxicity of the complexes, their cytotoxicity toward nontumoral cells (HDF and V79 fibroblasts) was evaluated. The in vivo cytotoxicity of complex 5 using the nematode Caenorhabditis elegans was also assessed. The silver camphorimine complexes displayed the highest selectivity coefficients (activity vs. toxicity).DF – Departamento de FísicaRUNCosta, Joana P.Pinheiro, TeresaMartins, Maria S.Carvalho, M. Fernanda N. N.Feliciano, JoanaLeitão, Jorge H.Silva, R. A. L.Guerreiro, Joana FAlves, Luís C.Custódio, InêsCruz, J.Marques, Fernanda2022-11-08T22:13:59Z2022-07-272022-07-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article21application/pdfhttp://hdl.handle.net/10362/145314engPURE: 46052620https://doi.org/10.3390/antibiotics11081010info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:25:33Zoai:run.unl.pt:10362/145314Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:00.514476Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Tuning the Biological Activity of Camphorimine Complexes through Metal Selection |
| title |
Tuning the Biological Activity of Camphorimine Complexes through Metal Selection |
| spellingShingle |
Tuning the Biological Activity of Camphorimine Complexes through Metal Selection Costa, Joana P. camphorimine caspase 3 caspase 7 cisplatin copper complex gold complex metal complex reactive oxygen metabolite unclassified drug agar gel electrophoresis apoptosis Article biological activity Caenorhabditis elegans cell viability clinical article cytotoxicity dispersity DPPH radical scavenging assay electrophoretic mobility elemental analysis fibroblast genetic transfection human human cell IC50 LC50 limit of detection lipid peroxidation lipophilicity MTT assay OVCAR-3 cell line oxidative stress ultraviolet spectrophotometry V79 cell line X ray X ray diffraction SDG 3 - Good Health and Well-being |
| title_short |
Tuning the Biological Activity of Camphorimine Complexes through Metal Selection |
| title_full |
Tuning the Biological Activity of Camphorimine Complexes through Metal Selection |
| title_fullStr |
Tuning the Biological Activity of Camphorimine Complexes through Metal Selection |
| title_full_unstemmed |
Tuning the Biological Activity of Camphorimine Complexes through Metal Selection |
| title_sort |
Tuning the Biological Activity of Camphorimine Complexes through Metal Selection |
| author |
Costa, Joana P. |
| author_facet |
Costa, Joana P. Pinheiro, Teresa Martins, Maria S. Carvalho, M. Fernanda N. N. Feliciano, Joana Leitão, Jorge H. Silva, R. A. L. Guerreiro, Joana F Alves, Luís C. Custódio, Inês Cruz, J. Marques, Fernanda |
| author_role |
author |
| author2 |
Pinheiro, Teresa Martins, Maria S. Carvalho, M. Fernanda N. N. Feliciano, Joana Leitão, Jorge H. Silva, R. A. L. Guerreiro, Joana F Alves, Luís C. Custódio, Inês Cruz, J. Marques, Fernanda |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
DF – Departamento de Física RUN |
| dc.contributor.author.fl_str_mv |
Costa, Joana P. Pinheiro, Teresa Martins, Maria S. Carvalho, M. Fernanda N. N. Feliciano, Joana Leitão, Jorge H. Silva, R. A. L. Guerreiro, Joana F Alves, Luís C. Custódio, Inês Cruz, J. Marques, Fernanda |
| dc.subject.por.fl_str_mv |
camphorimine caspase 3 caspase 7 cisplatin copper complex gold complex metal complex reactive oxygen metabolite unclassified drug agar gel electrophoresis apoptosis Article biological activity Caenorhabditis elegans cell viability clinical article cytotoxicity dispersity DPPH radical scavenging assay electrophoretic mobility elemental analysis fibroblast genetic transfection human human cell IC50 LC50 limit of detection lipid peroxidation lipophilicity MTT assay OVCAR-3 cell line oxidative stress ultraviolet spectrophotometry V79 cell line X ray X ray diffraction SDG 3 - Good Health and Well-being |
| topic |
camphorimine caspase 3 caspase 7 cisplatin copper complex gold complex metal complex reactive oxygen metabolite unclassified drug agar gel electrophoresis apoptosis Article biological activity Caenorhabditis elegans cell viability clinical article cytotoxicity dispersity DPPH radical scavenging assay electrophoretic mobility elemental analysis fibroblast genetic transfection human human cell IC50 LC50 limit of detection lipid peroxidation lipophilicity MTT assay OVCAR-3 cell line oxidative stress ultraviolet spectrophotometry V79 cell line X ray X ray diffraction SDG 3 - Good Health and Well-being |
| description |
the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-11-08T22:13:59Z 2022-07-27 2022-07-27T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/145314 |
| url |
http://hdl.handle.net/10362/145314 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
PURE: 46052620 https://doi.org/10.3390/antibiotics11081010 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
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21 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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