Genomic and proteomic characterization of the broad host range Salmonella phage PVP-SE1: the creation of a new phage genus

Detalhes bibliográficos
Autor(a) principal: Santos, Sílvio Roberto Branco
Data de Publicação: 2011
Outros Autores: Kropinski, Andrew M., Ceyssens, P. J., Ackermann, H. W., Villegas, A., Carvalho, Carla M., Lavigne, Rob, Krylov, V. N., Ferreira, Eugénio C., Azeredo, Joana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/16731
Resumo: (Bacterio)phage PVP-SE1, isolated from a German wastewater plant, presents a high potential value as a biocontrol agent and as a diagnostic tool, even compared to the well-studied typing phage Felix 01, due to its broad lytic spectrum against different Salmonella strains. Sequence analysis of its genome (145,964 bp) shows it to be terminally redundant and circularly permuted. Its G C content, 45.6 mol%, is lower than that of its hosts (50 to 54 mol%). We found a total of 244 open reading frames (ORFs), representing 91.6% of the coding capacity of the genome. Approximately 46% of encoded proteins are unique to this phage, and 22.1% of the proteins could be functionally assigned. This myovirus encodes a large number of tRNAs (n 24), reflecting its lytic capacity and evolution through different hosts. Tandem mass spectrometric analysis using electron spray ionization revealed 25 structural proteins as part of the mature phage particle. The genome sequence was found to share homology with 140 proteins of the Escherichia coli bacteriophage rV5. Both phages are unrelated to any other known virus, which suggests that an “rV5-like virus” genus should be created within the Myoviridae to contain these two phages.
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spelling Genomic and proteomic characterization of the broad host range Salmonella phage PVP-SE1: the creation of a new phage genusScience & Technology(Bacterio)phage PVP-SE1, isolated from a German wastewater plant, presents a high potential value as a biocontrol agent and as a diagnostic tool, even compared to the well-studied typing phage Felix 01, due to its broad lytic spectrum against different Salmonella strains. Sequence analysis of its genome (145,964 bp) shows it to be terminally redundant and circularly permuted. Its G C content, 45.6 mol%, is lower than that of its hosts (50 to 54 mol%). We found a total of 244 open reading frames (ORFs), representing 91.6% of the coding capacity of the genome. Approximately 46% of encoded proteins are unique to this phage, and 22.1% of the proteins could be functionally assigned. This myovirus encodes a large number of tRNAs (n 24), reflecting its lytic capacity and evolution through different hosts. Tandem mass spectrometric analysis using electron spray ionization revealed 25 structural proteins as part of the mature phage particle. The genome sequence was found to share homology with 140 proteins of the Escherichia coli bacteriophage rV5. Both phages are unrelated to any other known virus, which suggests that an “rV5-like virus” genus should be created within the Myoviridae to contain these two phages.A.M.K. is supported by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada. P.-J.C. holds a postdoctoral fellowship of the Fonds voor Wetenschappelijk Onderzoek (FWO) Vlaanderen. S.B.S is supported by grant SFRH/BD/32278/2006 from the Fundacao para a Ciencia e Tecnologia (FCT).American Society for MicrobiologyUniversidade do MinhoSantos, Sílvio Roberto BrancoKropinski, Andrew M.Ceyssens, P. J.Ackermann, H. W.Villegas, A.Carvalho, Carla M.Lavigne, RobKrylov, V. N.Ferreira, Eugénio C.Azeredo, Joana2011-11-042011-11-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/16731eng0022-538X10.1128/JVI.01769-1021865376info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:12:00Zoai:repositorium.sdum.uminho.pt:1822/16731Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:03:53.803930Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genomic and proteomic characterization of the broad host range Salmonella phage PVP-SE1: the creation of a new phage genus
title Genomic and proteomic characterization of the broad host range Salmonella phage PVP-SE1: the creation of a new phage genus
spellingShingle Genomic and proteomic characterization of the broad host range Salmonella phage PVP-SE1: the creation of a new phage genus
Santos, Sílvio Roberto Branco
Science & Technology
title_short Genomic and proteomic characterization of the broad host range Salmonella phage PVP-SE1: the creation of a new phage genus
title_full Genomic and proteomic characterization of the broad host range Salmonella phage PVP-SE1: the creation of a new phage genus
title_fullStr Genomic and proteomic characterization of the broad host range Salmonella phage PVP-SE1: the creation of a new phage genus
title_full_unstemmed Genomic and proteomic characterization of the broad host range Salmonella phage PVP-SE1: the creation of a new phage genus
title_sort Genomic and proteomic characterization of the broad host range Salmonella phage PVP-SE1: the creation of a new phage genus
author Santos, Sílvio Roberto Branco
author_facet Santos, Sílvio Roberto Branco
Kropinski, Andrew M.
Ceyssens, P. J.
Ackermann, H. W.
Villegas, A.
Carvalho, Carla M.
Lavigne, Rob
Krylov, V. N.
Ferreira, Eugénio C.
Azeredo, Joana
author_role author
author2 Kropinski, Andrew M.
Ceyssens, P. J.
Ackermann, H. W.
Villegas, A.
Carvalho, Carla M.
Lavigne, Rob
Krylov, V. N.
Ferreira, Eugénio C.
Azeredo, Joana
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Santos, Sílvio Roberto Branco
Kropinski, Andrew M.
Ceyssens, P. J.
Ackermann, H. W.
Villegas, A.
Carvalho, Carla M.
Lavigne, Rob
Krylov, V. N.
Ferreira, Eugénio C.
Azeredo, Joana
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description (Bacterio)phage PVP-SE1, isolated from a German wastewater plant, presents a high potential value as a biocontrol agent and as a diagnostic tool, even compared to the well-studied typing phage Felix 01, due to its broad lytic spectrum against different Salmonella strains. Sequence analysis of its genome (145,964 bp) shows it to be terminally redundant and circularly permuted. Its G C content, 45.6 mol%, is lower than that of its hosts (50 to 54 mol%). We found a total of 244 open reading frames (ORFs), representing 91.6% of the coding capacity of the genome. Approximately 46% of encoded proteins are unique to this phage, and 22.1% of the proteins could be functionally assigned. This myovirus encodes a large number of tRNAs (n 24), reflecting its lytic capacity and evolution through different hosts. Tandem mass spectrometric analysis using electron spray ionization revealed 25 structural proteins as part of the mature phage particle. The genome sequence was found to share homology with 140 proteins of the Escherichia coli bacteriophage rV5. Both phages are unrelated to any other known virus, which suggests that an “rV5-like virus” genus should be created within the Myoviridae to contain these two phages.
publishDate 2011
dc.date.none.fl_str_mv 2011-11-04
2011-11-04T00:00:00Z
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dc.language.iso.fl_str_mv eng
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10.1128/JVI.01769-10
21865376
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dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
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