Determination of Ketamine and Norketamine in hair samples using MEPS as sample clean-up

Detalhes bibliográficos
Autor(a) principal: Simão, Ana Y.
Data de Publicação: 2022
Outros Autores: Oliveira, Patrik, Rosendo, Luana M., Rosado, Tiago, Gallardo, Eugenia, Andraus, Maristela, Barroso, Mário
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.26/44097
Resumo: Palestra realizada no encontro SoHT-GTFI Joint Meeting, Verona, Junho 2022
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spelling Determination of Ketamine and Norketamine in hair samples using MEPS as sample clean-upKetamineHairMEPSPalestra realizada no encontro SoHT-GTFI Joint Meeting, Verona, Junho 2022Introduction and Aim: The use of hair samples to determine ketamine (K) and its metabolite, norketamine (NK), has been studied by several authors, using different clean-up approaches. However, microextraction by packed sorbent (MEPS), a miniaturized form of the classic solid-phase extraction has not been applied to date for the purpose. Therefore, a method to determine K and NK in hair samples was developed, optimized, and validated using MEPS as sample clean-up. Materials & Methods: Hair samples were scissor-cut into small fragments, and rinsed with methanol to remove dirt and externally deposited material. Following an overnight incubation with methanol at 65 ºC (no stirring), the compounds were analysed by GC-MS/MS without the need of derivatization procedures; MEPS conditions were: conditioning (5 x 250 µL of methanol and 4 x 250 µL of deionized water); load (10 x 150 µL of hair extract); washing (50 µL of 0.1% acetic acid and 50 µL of 10% methanol); and elution (100 µL of 3% ammonium hydroxide in methanol). Results & Discussion: The procedure resulted in acceptable recoveries, 39-61% for K and 32-43% for NK, and allowed reaching limits of quantification (LOQs) of 50 pg/mg for both analytes. The analytical method presented acceptable accuracy and precision with coefficients of variation typically lower than 15% and BIAS within ± 15%, except at the LOQ (20%). The method was successfully applied to 2 authentic samples, and ketamine concentrations were below 0.05 and 0.18 ng/mg. Norketamine was not detected. Conclusions: This work is the first analytical method using MEPS coupled to GC-MS/MS for the determination of K and NK in hair samples. Following a comparison with a SPE-based method using authentic samples, it was considered rapid and suitable for routine analysis. Acknowledgments: FCT (UIDB /00709/2020 and UIDP/00709/2020). A.Y. Simão acknowledges the PhD fellowship from FCT (2020.09070.BD).N/A2023-03-13T14:49:20Z2023-03-132022-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10400.26/44097http://hdl.handle.net/10400.26/44097engSimão, Ana Y.Oliveira, PatrikRosendo, Luana M.Rosado, TiagoGallardo, EugeniaAndraus, MaristelaBarroso, Márioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-03T04:22:29Zoai:comum.rcaap.pt:10400.26/44097Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:07:49.192795Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Determination of Ketamine and Norketamine in hair samples using MEPS as sample clean-up
title Determination of Ketamine and Norketamine in hair samples using MEPS as sample clean-up
spellingShingle Determination of Ketamine and Norketamine in hair samples using MEPS as sample clean-up
Simão, Ana Y.
Ketamine
Hair
MEPS
title_short Determination of Ketamine and Norketamine in hair samples using MEPS as sample clean-up
title_full Determination of Ketamine and Norketamine in hair samples using MEPS as sample clean-up
title_fullStr Determination of Ketamine and Norketamine in hair samples using MEPS as sample clean-up
title_full_unstemmed Determination of Ketamine and Norketamine in hair samples using MEPS as sample clean-up
title_sort Determination of Ketamine and Norketamine in hair samples using MEPS as sample clean-up
author Simão, Ana Y.
author_facet Simão, Ana Y.
Oliveira, Patrik
Rosendo, Luana M.
Rosado, Tiago
Gallardo, Eugenia
Andraus, Maristela
Barroso, Mário
author_role author
author2 Oliveira, Patrik
Rosendo, Luana M.
Rosado, Tiago
Gallardo, Eugenia
Andraus, Maristela
Barroso, Mário
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Simão, Ana Y.
Oliveira, Patrik
Rosendo, Luana M.
Rosado, Tiago
Gallardo, Eugenia
Andraus, Maristela
Barroso, Mário
dc.subject.por.fl_str_mv Ketamine
Hair
MEPS
topic Ketamine
Hair
MEPS
description Palestra realizada no encontro SoHT-GTFI Joint Meeting, Verona, Junho 2022
publishDate 2022
dc.date.none.fl_str_mv 2022-06-01T00:00:00Z
2023-03-13T14:49:20Z
2023-03-13
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.26/44097
http://hdl.handle.net/10400.26/44097
url http://hdl.handle.net/10400.26/44097
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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