Thermosensitive polymeric matrices for three-dimensional cell culture strategies
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/12923 |
Resumo: | A completely new strategy for cell culture focusing on the design of three-dimensional (3D) smart surfaces by supercritical fluid technology has been developed. This approach might overcome the limitations on cell expansion and proliferation of currently existing techniques. An alternative technology, based on supercritical carbon dioxide, was used to polymerize poly(N-isopropylacrylamide) (PNIPAAm) and to foam poly(d,l-lactic acid) (PD,LLA), creating a thermosensitive 3D structure which has proven to have potential as a substrate for cell growth and expansion. We demonstrated that the thermosensitive matrices promoted cell detachment, thus PD,LLA scaffolds have the potential to be used as substrates for cell growth and expansion avoiding enzymatic and mechanical methods of cell harvesting. The harvested cells were replated to evaluate their viability, which was not compromised. A major advantage of this technology is the fact that the prepared materials can be recovered and reused. Therefore, the same substrate can be recycled and reused for different batches. An indirect impact of the technology developed is related to the field of biotechnology, as this novel technology for cell expansion can be applied to any adherent cell cultures. |
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Thermosensitive polymeric matrices for three-dimensional cell culture strategiesSupercritical fluidsCell expansionTissue engineeringThermoresponsive matricesPoly-lactic acidScience & TechnologyA completely new strategy for cell culture focusing on the design of three-dimensional (3D) smart surfaces by supercritical fluid technology has been developed. This approach might overcome the limitations on cell expansion and proliferation of currently existing techniques. An alternative technology, based on supercritical carbon dioxide, was used to polymerize poly(N-isopropylacrylamide) (PNIPAAm) and to foam poly(d,l-lactic acid) (PD,LLA), creating a thermosensitive 3D structure which has proven to have potential as a substrate for cell growth and expansion. We demonstrated that the thermosensitive matrices promoted cell detachment, thus PD,LLA scaffolds have the potential to be used as substrates for cell growth and expansion avoiding enzymatic and mechanical methods of cell harvesting. The harvested cells were replated to evaluate their viability, which was not compromised. A major advantage of this technology is the fact that the prepared materials can be recovered and reused. Therefore, the same substrate can be recycled and reused for different batches. An indirect impact of the technology developed is related to the field of biotechnology, as this novel technology for cell expansion can be applied to any adherent cell cultures.FCT project PTDC/QUI/68804/2006; grant SFRH/BPD/34994/2007ElsevierUniversidade do MinhoDuarte, Ana Rita C.Mano, J. F.Reis, R. L.20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/12923eng1742-706110.1016/j.actbio.2010.09.03220883833http://www.sciencedirect.com/science/article/pii/S1742706110004423info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:28:57Zoai:repositorium.sdum.uminho.pt:1822/12923Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:23:51.553135Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Thermosensitive polymeric matrices for three-dimensional cell culture strategies |
title |
Thermosensitive polymeric matrices for three-dimensional cell culture strategies |
spellingShingle |
Thermosensitive polymeric matrices for three-dimensional cell culture strategies Duarte, Ana Rita C. Supercritical fluids Cell expansion Tissue engineering Thermoresponsive matrices Poly-lactic acid Science & Technology |
title_short |
Thermosensitive polymeric matrices for three-dimensional cell culture strategies |
title_full |
Thermosensitive polymeric matrices for three-dimensional cell culture strategies |
title_fullStr |
Thermosensitive polymeric matrices for three-dimensional cell culture strategies |
title_full_unstemmed |
Thermosensitive polymeric matrices for three-dimensional cell culture strategies |
title_sort |
Thermosensitive polymeric matrices for three-dimensional cell culture strategies |
author |
Duarte, Ana Rita C. |
author_facet |
Duarte, Ana Rita C. Mano, J. F. Reis, R. L. |
author_role |
author |
author2 |
Mano, J. F. Reis, R. L. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Duarte, Ana Rita C. Mano, J. F. Reis, R. L. |
dc.subject.por.fl_str_mv |
Supercritical fluids Cell expansion Tissue engineering Thermoresponsive matrices Poly-lactic acid Science & Technology |
topic |
Supercritical fluids Cell expansion Tissue engineering Thermoresponsive matrices Poly-lactic acid Science & Technology |
description |
A completely new strategy for cell culture focusing on the design of three-dimensional (3D) smart surfaces by supercritical fluid technology has been developed. This approach might overcome the limitations on cell expansion and proliferation of currently existing techniques. An alternative technology, based on supercritical carbon dioxide, was used to polymerize poly(N-isopropylacrylamide) (PNIPAAm) and to foam poly(d,l-lactic acid) (PD,LLA), creating a thermosensitive 3D structure which has proven to have potential as a substrate for cell growth and expansion. We demonstrated that the thermosensitive matrices promoted cell detachment, thus PD,LLA scaffolds have the potential to be used as substrates for cell growth and expansion avoiding enzymatic and mechanical methods of cell harvesting. The harvested cells were replated to evaluate their viability, which was not compromised. A major advantage of this technology is the fact that the prepared materials can be recovered and reused. Therefore, the same substrate can be recycled and reused for different batches. An indirect impact of the technology developed is related to the field of biotechnology, as this novel technology for cell expansion can be applied to any adherent cell cultures. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2011-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/12923 |
url |
http://hdl.handle.net/1822/12923 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1742-7061 10.1016/j.actbio.2010.09.032 20883833 http://www.sciencedirect.com/science/article/pii/S1742706110004423 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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