Unravelling phytochemical and bioactive potential of three hypericum species from romanian spontaneous flora: H. alpigenum, H. perforatum and H. rochelii

• Autor(a) principal: Babotă, Mihai
• Data de Publicação: 2022
• Outros Autores: Frumuzachi, Oleg, Mocan, Andrei, Tamas, Mircea, Dias, Maria Inês, Pinela, José, Stojković, Dejan, Soković, Marina, Badarau, Alexandru Sabin, Crisan, Gianina, Barros, Lillian, Paltinean, Ramona
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10198/27452
• topic: Hypericum species; Hypericum rochelii; Hypericum alpigenum; Antioxidant activity; Enzyme inhibition

Hypericum perforatum L., also known as St. John's Wort, is recognized worldwide as a valuable medicinal herb; however, other Hypericum species were intensively studied for their bioactive potential. To fill the research gap that exists in the scientific literature, a comparative evaluation between H. alpigenum Kit., H. perforatum L. and H. rochelii Griseb. & Schenk was conducted in the present study. Two types of herbal preparations obtained from the aerial parts of these species were analyzed: extracts obtained through maceration and extracts obtained through magnetic-stirring-assisted extraction. LC-DAD-ESI-MSn analysis revealed the presence of phenolic acids, flavan-3-ols and flavonoid derivatives as the main constituents of the above-mentioned species. Moreover, all extracts were tested for their antioxidant, enzyme-inhibitory and antimicrobial potential. Our work emphasizes for the first time a detailed description of H. rochelii phenolic fractions, including their phytochemical and bioactive characterization. In comparison with the other two studied species, H. rochelii was found as a rich source of phenolic acids and myricetin derivatives, showing important antioxidant, anticholinesterase and antibacterial activity. The study offers new perspectives regarding the chemical and bioactive profile of the less-studied species H. alpigenum and H. rochelii.