Eimeria vermiformis Infection model of Murine small intestine

Detalhes bibliográficos
Autor(a) principal: Figueiredo-Campos, Patricia
Data de Publicação: 2018
Outros Autores: Ferreira, Cristina, Blankenhaus, Birte, Veldhoen, Marc
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/49885
Resumo: Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.
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spelling Eimeria vermiformis Infection model of Murine small intestineEimeria vermiformisSmall intestineInfection modelCoccidiosisApicomplexanMucosal immunologyCopyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.Eimeria vermiformis is a tissue specific, intracellular protozoan that infects the murine small intestinal epithelia, which has been widely used as a coccidian model to study mucosal immunology. This mouse infection model is valuable to investigate the mechanisms of host protection against primary and secondary infection in the small intestine. Here, we describe the generation of an E. vermiformis stock solution, preparation of sporulated E. vermiformis to infect mice and determination of oocysts burden. This protocol should help to establish a highly reproducible natural infection challenge model to study immunity in the small intestine. The information obtained from using this mouse model can reveal fundamental mechanisms of interaction between the pathogen and the immune response, e.g., provided by intraepithelial lymphocytes (IEL) at the basolateral site of epithelial cells but also a variety of other immune cell populations present in the gut.This work was supported by the European Union H2020 ERA project (No. 667824– EXCELLtoINNOV) for work in the Veldhoen laboratory and Fundação para a Ciência e a Tecnologia (FCT) Studentship SFRH/BD/131605/2017 to P.F-C.Bio-protocol LLCRepositório da Universidade de LisboaFigueiredo-Campos, PatriciaFerreira, CristinaBlankenhaus, BirteVeldhoen, Marc2021-10-14T11:02:00Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/49885engBio Protoc. 2018 Dec 20;8(24):e312210.21769/BioProtoc.31222331-8325info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:53:50Zoai:repositorio.ul.pt:10451/49885Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:24.639640Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Eimeria vermiformis Infection model of Murine small intestine
title Eimeria vermiformis Infection model of Murine small intestine
spellingShingle Eimeria vermiformis Infection model of Murine small intestine
Figueiredo-Campos, Patricia
Eimeria vermiformis
Small intestine
Infection model
Coccidiosis
Apicomplexan
Mucosal immunology
title_short Eimeria vermiformis Infection model of Murine small intestine
title_full Eimeria vermiformis Infection model of Murine small intestine
title_fullStr Eimeria vermiformis Infection model of Murine small intestine
title_full_unstemmed Eimeria vermiformis Infection model of Murine small intestine
title_sort Eimeria vermiformis Infection model of Murine small intestine
author Figueiredo-Campos, Patricia
author_facet Figueiredo-Campos, Patricia
Ferreira, Cristina
Blankenhaus, Birte
Veldhoen, Marc
author_role author
author2 Ferreira, Cristina
Blankenhaus, Birte
Veldhoen, Marc
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Figueiredo-Campos, Patricia
Ferreira, Cristina
Blankenhaus, Birte
Veldhoen, Marc
dc.subject.por.fl_str_mv Eimeria vermiformis
Small intestine
Infection model
Coccidiosis
Apicomplexan
Mucosal immunology
topic Eimeria vermiformis
Small intestine
Infection model
Coccidiosis
Apicomplexan
Mucosal immunology
description Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
2021-10-14T11:02:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/49885
url http://hdl.handle.net/10451/49885
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bio Protoc. 2018 Dec 20;8(24):e3122
10.21769/BioProtoc.3122
2331-8325
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Bio-protocol LLC
publisher.none.fl_str_mv Bio-protocol LLC
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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